LOCUS BE516617 556 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0630_A06_A12ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0630_A06_A12, mRNA sequence. ACCESSION BE516617 VERSION BE516617.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 556) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..556 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0630_A06_A12" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 151 a 152 c 128 g 125 t ORIGIN 1 tcgcggccgc gtcgacggag tttgaatgtt tggcaacacg ttctgcggga aaaacatgag 61 gttttatcca tgtgagaggt acaggaccga ggcaagcttc ttccagaacc ctgcaatctc 121 acaatgccat tgaataggtc tctcatttct cgacgaccag caatatcgac agagtcaagg 181 aacattccag catcagcgag gcacttgact ctggtatttg atggaaacat tccacggaac 241 tcatcacagt gaagtatggc agaagcaccc ccagcagagc atccagaaag aagggcctga 301 ctagcagaat gcattccttg ggccatcaag tcatccatag cagcctgcca aatacgctga 361 cctcggaaat aaaggcctga agccgcgtca gccccatcac cggtgaagga tccaccatca 421 caataccgaa ccttcactct gttccagttg tagaaatcag gattttcttc aggtctgtta 481 ctcattatgc ctgtaaactg gagttgcctc tccatgtggt ttgatgaccc acgccgacta 541 cccttgcgga acacgc //