LOCUS BE516596 488 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0630_C08_E16ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0630_C08_E16, mRNA sequence. ACCESSION BE516596 VERSION BE516596.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 488) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..488 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0630_C08_E16" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 121 a 108 c 109 g 150 t ORIGIN 1 tcgcggccgc gtcgactttt tttttttttt tttttttttt tccggttgct taaaaacact 61 tctattcagg atgtctcaac gttcgtcaat tctgaggatg ttctgttgtc aaattttatg 121 tacaaagact tcggtacttt acaacgtcta tacagtatta taaataggga gacccaggtc 181 acggcagggt attgactgtt acacgctaac atcacctcag caccaaatgt gtactacctt 241 ggtccggcgg tattttctgc aggtcacggc agggtattat ctgcagtacc aacgcacttg 301 tcgatttgtc agcagaatgg aaaacggtat gtccagtggt atgagtgaac caagttggtt 361 gcagaaaacc tcagttcagc gcatagtgga cccgctccaa cattcgcatg acctggaaaa 421 cggagataga cctgtgtggt gcctttactg aagacccatc atcaagaaca gtttgctgtt 481 gctcactg //