LOCUS BE516568 550 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0630_F09_K18ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0630_F09_K18, mRNA sequence. ACCESSION BE516568 VERSION BE516568.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 550) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..550 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0630_F09_K18" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 118 a 144 c 126 g 162 t ORIGIN 1 tcgcggccgc gtcgaccgct attggagctg gaattaccgc ggctgctggc accagacttg 61 ccctccaatg gatcctcgtt aagggattta gattgtactc attccaatta ccagacacta 121 atgcgcccgg tattgttggt gcttcgttcg ggcgcaatga tggtgtcctg ctagattgta 181 gctcttctac cctctgttct agctctctga gataagttat agtttctgca aggatggatg 241 ctttgtccat cttgttaata gacgggatca ttgacttgag aactaggaac atatcattga 301 gcttctcccg acgccttctt tccgacagga catgtgtctt gatgttactt tcttgaggtc 361 tcgctatcca tgcaccaccg gctacagctt tcttcaacaa cttttgtgac tccccggtga 421 cccgcgcaac cacgtgttcc cagtcagatg acctcttcca caccgtaaaa catgatggat 481 gagagccttt aacaaagcta cttaaagtga tgacggcatc gtcaatgctt ggtgcatcca 541 tgtctggtgc //