LOCUS       BE516511                 471 bp    mRNA    linear   EST 08-AUG-2000
DEFINITION  WHE611_D09_H17ZA Wheat ABA-treated embryo cDNA library Triticum
            aestivum cDNA clone WHE611_D09_H17, mRNA sequence.
VERSION     BE516511.1
DBLINK      BioSample: SAMN00160090
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y.,
            Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C.,
            Verhey,S.D. and Walker-Simmons,M.K.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - ABA-treated embryo library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Clontech Matchmaker 3' AD primer.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Triticum aestivum"
                     /cultivar="Brevor (soft, white, winter, common wheat)"
                     /tissue_type="Seed embryo"
                     /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA
                     /dev_stage="Mature dormant seeds"
                     /lab_host="E. coli DH12S"
                     /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI;
                     Embryos were cut from mature, dormant seeds and imbibed in
                     25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7,
                     for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA
                     were prepared by Steven Verhey in M.K. Walker-Simmons's
                     lab (USDA-ARS, Washington State Univ., Pullman, Washington
                     99164-6420. A cDNA library was made by Clontech using a
                     combination of random and oligo dT primers. Library was
                     plated and archived by Russell Johnson (Colby College,
                     ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA
                     sequencing were performed in the OD Anderson lab (all
                     other authors)."
BASE COUNT          124 a           97 c          124 g          126 t
        1 tcgcggccgc gtcgacctca gtacaagaag ggtaaggaca gtctgtctgc ccagggaaag
       61 cgtcgttatg acaggaagca gtcgggatat ggtggtcaga ccaagcctgt tttccacaag
      121 aaggcaaaaa ccacaaagaa gattgtgctg aagctgcaat gccagagctg caagcactac
      181 tcccagcgtg ccatcaagag gtgcaagcat tttgagatcg gtggagacaa gaagggcaag
      241 gggacatctc ttttctagac tgctttcacc tatctggagt ggtggtggtc aagaagtatt
      301 actacttgtg ttaagagttt gttgttagga gttaaattaa tcagaccttg tcatgtatcc
      361 aaactcctgt gtctcgcatt taagcaagag gcgacttttg gctaagtgaa gttttgtagc
      421 ttgaatcctc tttcattccg tataatgtct ttattttagt cgacgcggcc g