LOCUS BE516464 659 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE610_B01_C02ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE610_B01_C02, mRNA sequence. ACCESSION BE516464 VERSION BE516464.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 659) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..659 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE610_B01_C02" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 183 a 145 c 140 g 191 t ORIGIN 1 tcgcggccgc gtcgactcac gattgaaaat aagacaaagg ccgaggtgag gagcggccag 61 taccatactt aactcataca gtatattgca ctcatttgag ttcttttctc taataaactc 121 agtaaaggcc ggatttgatc attctgacca ctactatgtc catttgatac taattctata 181 tgcttgggag atctgtctgt tgatgacctt cctccagaaa cactaagccg ctgtggcctt 241 accaggtcat tatcatttct ctccatgtga ccagcagcag atatatcatt gaacctagga 301 gatgctgatg gatccaataa gatatggtgc ttatcagcat ctgcttgtaa tgactgcatg 361 gagccagatg aatggtaatg atccagcctt actttagaac tttctagcat cggcacatct 421 agctgaccag attttagacg ggggggtgga accattctgt actgaagcac ctgaccctga 481 gatggaagca aatcgtgttg cttcattcaa gctatgaaga gtattaacta gcctgagaag 541 tatcccattt tttgctgcta tacgacagaa atcatttctt ggcgttgaat gctgaagctt 601 gaagacctgc cagatgccat caattgcaag atgaaccatt tccctaaatt ttgcatagt //