LOCUS BE516410 522 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE610_G11_M22ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE610_G11_M22, mRNA sequence. ACCESSION BE516410 VERSION BE516410.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 522) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..522 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE610_G11_M22" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 61 a 184 c 179 g 98 t ORIGIN 1 cgcggccgcg tcgaccgtag tacgccgcga agtcccggaa cggctgcgcg ccgccgccgc 61 cgccgttggc gttcatcccc atgacgggct tctgctccgg cggggagctc gccatggccc 121 cagggccacc cccgtagccg cccatggccg ccggcttccg gtccacggcc gccggcttct 181 ccatgccgcc cttcttgttg tagatgcggc acagcaccca atcatccaac ctgaggctgt 241 tcttcttgcg ggcggagcgg tcgacgtcgg cgaggcggta ctcgtgcatg atccagttgg 301 tcttctcgcc cttgggggcc ttgccggcgt agaagacgag cgccttcttg atggcgagcg 361 gcttgggggt gcccacgggc ttgtcggccc cggtggcctt ccagtagccg gacccggcgg 421 cccggttggg cctggacccg ttggggtact tgcggtcccg cggggagaag aagtaccact 481 ccttctcgcc gtacagcgcc atctttggga gctgccaggg gt //