LOCUS BE516326 549 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE617_H12_O23ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE617_H12_O23, mRNA sequence. ACCESSION BE516326 VERSION BE516326.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 549) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..549 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE617_H12_O23" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 150 a 139 c 126 g 134 t ORIGIN 1 tcgcggccgc gtcgactttt tttttttttt ttttggagac caagatggta atctatttag 61 tagcaatcct gaatgcacct caaacacacc agatctgaaa tattaagttg acagacaaaa 121 atgccgaaaa ggggaaggca gagcgttcac aaaaggaatg aaacgagcag ctcagctcag 181 ctcatacata tgccagtcac taccacatag cgagctcact gcaccatctc catgtccttg 241 aagtactcat gctcaagagc ctgcctggcc gtgatcctct tgtttggctc gaaccgaagc 301 attttcgaga gaaggtccag gccaacaggt tcaagattgg ggacaacggt tgcaaggtcc 361 tctgcctgcc acctggggaa ggcggacttg tagtcaggca aggagctcac gcctggccaa 421 gtttgttcat ttggagtgcc cagtaccctg aatatcttaa atagttcatc aatctcaaaa 481 tcgccaggga acagtggttt ctggttcacc atttctgcaa gatacagccc actgaccaca 541 cgtcaactg //