LOCUS BE516323 531 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE617_H09_O17ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE617_H09_O17, mRNA sequence. ACCESSION BE516323 VERSION BE516323.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 531) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..531 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE617_H09_O17" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 122 a 133 c 118 g 157 t ORIGIN 1 tcgcggccgc gtcgactttt tttttttttt taaccgatca ggccatcaaa ttggcgggtt 61 atatgaatcc tcatgaaaag ttagcaaaca taaaatttct aagctctagg tacaccaatc 121 agtttacaca ttcttctcgg caacgagcca caaataatac atgactgcga cggggcaact 181 ctacagggcc cccggtgagc tgtgagaagt ggactaaaac atcatctttg ctaaaagcac 241 actgaatcga tgatgggtgn ttgtggttag ataaaggaac accacatctc atctctgtca 301 ctccctcgtg gggtctctca cctagggtta gcctccagtt tgtggctgcc acctacgtac 361 ttcccgttgc tgttattatt agccttctta tgaagcttac cctcatcatc gtgatgctgc 421 actttgggcg ctgcgatgct catgcccagt gtggttgtga ccgcggcagg ttcctgagac 481 attagttgtg cattctgtgc ttccatatct tcacaggcct tcttaagctg g //