LOCUS BE516163 508 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE612_G08_N16ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE612_G08_N16, mRNA sequence. ACCESSION BE516163 VERSION BE516163.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 508) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..508 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE612_G08_N16" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 114 a 148 c 140 g 106 t ORIGIN 1 tcgcggccgc gtcgactggc tatggaggtt ctgtacccgc tcttcctctg cctcgccgtg 61 ctcgccggca cggcagcctc cgccccacca cactcgcagt gcctagacca cccaccggac 121 ttcacggccg ccagggtcga gtccggaaag gtcgtctatg acttcgccgg ctacaaggcg 181 tacgtcaccg gcgccatcca ctccgaccgc gccgtcgtcc tggcctccga cgtctttgga 241 ttcgaagcgc cgttgctgag gaagatagca gacaaagttg gtgaagctgg atactatgtc 301 gtggtccctg atttctttaa tgggcaacct ttaacgggga caccaggtga aaacctcacc 361 aaatggctca acgaacactc tccggtaaaa gctgctcaag atgccaaacc aatatttgcg 421 accttgagaa aagagcgaaa atttagtctt ggagttggcg gatactgttg gggtggaaag 481 ttcgcagtgg agatagcgaa aatgaatg //