LOCUS BE516141 577 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE612_E06_J12ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE612_E06_J12, mRNA sequence. ACCESSION BE516141 VERSION BE516141.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 577) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..577 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE612_E06_J12" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 154 a 143 c 113 g 167 t ORIGIN 1 tcgcggccgc gtcgactcga attccagcat atgttgtccc gttaaccgat ctcccgagta 61 cttcctgcag gaacaatttg tcgtttgaaa ctactgcagc tgtcagatgc gcattcatcc 121 tttcgcaggc ttctaatacc aaatcaagct gttcctcaga gtattctgta accacctgga 181 aaggaccaaa tatctccttt gtcacaagct cgaagttacc atttttaagt atctccacta 241 gtgggacaaa tacagcagtc ggtttgaaag caccatatat ttctgggatg gagtgattct 301 ccaaaggttc accaccaaac agaacctttg atcctggtat tttgagaagg ttgttcatgt 361 gctctatcat ggttgctgtt gtaacagtaa ggactgggcc aatcgtcaaa tcttcaagct 421 tccttctttc agaaagtttc ttcattttct caagtagccc actagatgac cagttcttgt 481 gcatgaatag catagactga gcagagcact tctgaccgct gcaagcgtaa gcatcctggt 541 cgcaaaccca tgaaatataa tcaacctctt gaacatc //