LOCUS BE516019 627 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0607_H11_P21ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0607_H11_P21, mRNA sequence. ACCESSION BE516019 VERSION BE516019.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 627) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..627 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0607_H11_P21" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 138 a 197 c 208 g 84 t ORIGIN 1 tcgcggccgc gtcgacaaca cacttgcaca gccacacgta tcacccgtag atcgatcact 61 gttcgtacca ccccaatgga gtatcagggg cagcacggcc acgccaccga aaggtggagg 121 agtacggcca gcccgtggcc gggcacggcg gtttcaccgg cgggcccacg gggacgcacg 181 gcgccgccgg caccggcgcg cagctccagg cgacgcggga cgaccacaag accgacggcg 241 tcctgcgccg ctccggcagc tccagctcca gctcatctga ggacgacggc gtgggcggca 301 ggaggaagaa ggggatgaag gagaagatca aggagaagct ccccggcgga gcccacaagg 361 acgccaccgc cgggcagcag cacacggcgg tggcgggcga gtacgcggga acgcacagca 421 ccgaggccac cggcgagaag aagggcgtca tggacaagat caaggagaag cttcccggcg 481 gacagcactg agccagctat gcgtgtcttc tctatgttcc gtcaaagctc ggctagcatc 541 tgcagccatg ccgttactac ggatagataa tggactctgt cactctgata taccttgtgt 601 gcatgagcga gcccgtgtgt gttcgta //