LOCUS BE515973 689 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0607_C10_F19ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0607_C10_F19, mRNA sequence. ACCESSION BE515973 VERSION BE515973.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 689) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..689 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0607_C10_F19" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 164 a 155 c 197 g 172 t ORIGIN 1 tcgcggccgc gtcgacctcc gtctcgcccg cgtgttgggg taggagccat ggcggaccag 61 ctcaccgacg accagatcgc cgagttcaag gaggccttca gcctcttcga caaggacgga 121 gacggctgca tcaccaccaa ggagcttgga actgtgatgc gctcgctggg gcagaacccc 181 actgaggcag agcttcagga tatgatcaat gaagtggatg ctgatggcaa tggaacgatt 241 gactttcctg agttcctcaa cctgatggca cgtaagatga aggacactga ttccgaggag 301 gagcttaagg aggctttccg tgtgtttgac aaggaccaga atggtttcat ctcggcagct 361 gaactccgcc atgtcatgac caaccttggt gagaagctga cagacgagga ggtggatgag 421 atgatccgtg aggctgacgt ggatggtgac ggccanatca attacgaaga gtttgtgaag 481 gtgatgatgg ccaagtgagc tgccatacta agctagttct ctctttttag atgaactaag 541 ctagttctct cttgatgtta gcagctagtt tagccttgaa aactgacagc actcgttact 601 actgttaagt ccagtgaatg aacatattgc gcgtgcttcc ttctcaggga tgttgagaat 661 ttcgtttgga ttttcttgca gttgtatta //