LOCUS BE515726 462 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0602_A06_A12ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0602_A06_A12, mRNA sequence. ACCESSION BE515726 VERSION BE515726.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 462) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..462 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0602_A06_A12" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 124 a 131 c 88 g 119 t ORIGIN 1 tcgcggccgc gtcgacgaat tccgaacctc cggacggtca tctgcaccca atttttgaag 61 gacggaaaaa actgaaaaga acagcttatt gtaatcaacc agttgttgca agggatttcg 121 cttgacgcct tcctcactgg ttggtatatc tccctcttcc gttgatacac ccaactgaac 181 ctcttcattc aagtgattag ctttctgaac agacctgcca cttaacccct tcacaataaa 241 atcggtcgca gtccacaaaa gaccagctgc tgtcaaactt atgtttatgt cagttttctg 301 ggcaccatac gccccagtaa ccaaaatgca ctcatcaaga cattgtacag gtattgtcgc 361 cagtccttca ttcatgatca cacgtacact ctggaaacct aaggagatga catctttttc 421 agatgcatcg gcaacagccc tcagcattcg tcgacgcggc cg //