LOCUS BE515695 566 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0602_D10_G20ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0602_D10_G20, mRNA sequence. ACCESSION BE515695 VERSION BE515695.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 566) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..566 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0602_D10_G20" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 149 a 154 c 127 g 136 t ORIGIN 1 tcgcggccgc gtcgactttt tttttttttt tttttagggg ccacaacaga gataaatccg 61 atcaaaagct ctttcaggtt caaactcacc aaatctcagt agccaaacca cagtgcaaaa 121 tcttaagaaa gctaacagac cataatccag cagcattaca taatccagga aaagtgccac 181 aagtaataag ctagaagaaa agagggacat gctatcaaag ctttgatcat ctagctgctc 241 cagcgatcag atcagacgat ggcattcgcg gcgctggcaa tcctgggcca caggtcggcg 301 cactccttcc cgatcggtcc agtgatggca gaacctttca tctctccctt tgggttcaca 361 atgactccag cattgtcttc aaagtacatg aagacaccgt cctttcggcg ccatggcttg 421 cgctgcctga cgatgacagc cggcatgacc ttcttcctga ggtcaggctt tcccttcttg 481 acagtggcca taaccatgtc accaacgcag gcggaaggaa gcctgttgag gcgccccttg 541 attcccttga cggagatgat gtacag //