LOCUS       BE515472                 454 bp    mRNA    linear   EST 08-AUG-2000
DEFINITION  WHE0613_D03_G05ZA Wheat ABA-treated embryo cDNA library Triticum
            aestivum cDNA clone WHE0613_D03_G05, mRNA sequence.
ACCESSION   BE515472
VERSION     BE515472.1
DBLINK      BioSample: SAMN00160090
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y.,
            Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C.,
            Verhey,S.D. and Walker-Simmons,M.K.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - ABA-treated embryo library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Clontech Matchmaker 3' AD primer.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Brevor (soft, white, winter, common wheat)"
                     /db_xref="taxon:4565"
                     /clone="WHE0613_D03_G05"
                     /tissue_type="Seed embryo"
                     /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA
                     library"
                     /dev_stage="Mature dormant seeds"
                     /lab_host="E. coli DH12S"
                     /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI;
                     Embryos were cut from mature, dormant seeds and imbibed in
                     25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7,
                     for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA
                     were prepared by Steven Verhey in M.K. Walker-Simmons's
                     lab (USDA-ARS, Washington State Univ., Pullman, Washington
                     99164-6420. A cDNA library was made by Clontech using a
                     combination of random and oligo dT primers. Library was
                     plated and archived by Russell Johnson (Colby College,
                     ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA
                     sequencing were performed in the OD Anderson lab (all
                     other authors)."
BASE COUNT          115 a          120 c          107 g          112 t
ORIGIN      
        1 tcgcggccgc gtcgacggcg tgcaagatag ctaagtaggt aagcaaagcc tgttcaggtt
       61 cctcattcca agtcctgtgg aaaataaatg atagcaaccg ttgcttgata ccgggaagaa
      121 aagaactcca ttgtgcagcc tggccatgtg aatgaagaca tagctcgtca tcttccgaca
      181 aaatgcacca attcttcgta cctcaacaag gctgcttgaa tctcggattt gtagaacatg
      241 tattgtttga tatctcacaa gaaggaaagt tattttctca attgatggca caccgtgact
      301 tgaccgctgc tccacaagca acgcccatgg acctggctgc tgctcatgtc gtgtgtgcag
      361 gctgctacac tacccgacgg cctacatcgc cacgtatact tgcttcacac aatcgcacgg
      421 ggctcgtcag ctagactcgg cttcgagttc gtgc
//