LOCUS BE515472 454 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0613_D03_G05ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0613_D03_G05, mRNA sequence. ACCESSION BE515472 VERSION BE515472.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 454) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..454 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0613_D03_G05" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 115 a 120 c 107 g 112 t ORIGIN 1 tcgcggccgc gtcgacggcg tgcaagatag ctaagtaggt aagcaaagcc tgttcaggtt 61 cctcattcca agtcctgtgg aaaataaatg atagcaaccg ttgcttgata ccgggaagaa 121 aagaactcca ttgtgcagcc tggccatgtg aatgaagaca tagctcgtca tcttccgaca 181 aaatgcacca attcttcgta cctcaacaag gctgcttgaa tctcggattt gtagaacatg 241 tattgtttga tatctcacaa gaaggaaagt tattttctca attgatggca caccgtgact 301 tgaccgctgc tccacaagca acgcccatgg acctggctgc tgctcatgtc gtgtgtgcag 361 gctgctacac tacccgacgg cctacatcgc cacgtatact tgcttcacac aatcgcacgg 421 ggctcgtcag ctagactcgg cttcgagttc gtgc //