LOCUS BE515380 660 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0605_G01_M01ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0605_G01_M01, mRNA sequence. ACCESSION BE515380 VERSION BE515380.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 660) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..660 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0605_G01_M01" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 124 a 231 c 156 g 149 t ORIGIN 1 cggacttcat gaactacctc ggccactgca acttcgagct cgtccccaag ctcctcttcg 61 acctcttccc gccgctcaag tacctcatgt acacgccatc gttccactcg ctgcaccaca 121 cgcagttcag gaccaactac tcgctcttcg tgcccttcta cgactacgtg tacggcacca 181 tggacaagtc gagcgacgac ctgtacgagc gcacgctgca cggcgggagg aggcccccga 241 cgtcgtccac ctcacccatc tcaccgcgcc gggttccctc ctccacctcc gcctcggctt 301 cgcctccctc gcctccgcgc cgctcgactc gcgccccccg ttggcgctgg cgtgggcact 361 ggcgatggtg gagcggccgg tcgccgcgct ggcctcgctg ctggtttttt tttttttcaa 421 atctaagaaa ccagtcagtt caagacctgt ctcaactttt gattacatcc aacttgcgcg 481 gcaaaggcag tcgtcccaca acatataaaa ggtagaaaca catgatgttc tatatcgtcg 541 ggtccggggg tgacaaactc cgccatggta caagaaaaca gacagtgttt cgtttcgcct 601 gacagtctcc tactctcatc ttagtccttg ccaaagtcgg cgctgtagtg tgcgctggac //