LOCUS       BE455654                 587 bp    mRNA    linear   EST 22-OCT-2001
DEFINITION  HVSMEg0018J22f Hordeum vulgare pre-anthesis spike EST library
            HVcDNA0008 (white to yellow anther) Hordeum vulgare subsp. vulgare
            cDNA clone HVSMEg0018J22f, mRNA sequence.
ACCESSION   BE455654
VERSION     BE455654.2
DBLINK      BioSample: SAMN00159197
KEYWORDS    EST.
SOURCE      Hordeum vulgare subsp. vulgare (domesticated barley)
  ORGANISM  Hordeum vulgare subsp. vulgare
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Wing,R., Close,T.J., Kleinhofs,A., Wise,R., Begum,D., Frisch,D.,
            Yu,Y., Henry,D., Palmer,M., Rambo,T., Simmons,J., Choi,D.W.,
            Fenton,R.D., Close,S.J., Oates,R. and Main,D.
  TITLE     Development of a genetically and physically anchored EST resource
            for barley genomics: Morex pre-anthesis spike cDNA library
  JOURNAL   Unpublished
COMMENT     On Jul 26, 2000 this sequence version replaced BE455654.1.
            Contact: Wing RA
            Clemson University Genomics Institute
            Clemson University
            100 Jordan Hall, Clemson, SC 29634, USA
            Tel: 864 656 7288
            Fax: 864 656 4293
            Email: rwing@clemson.edu
            Total hq bases = 363
            Seq primer: AATTAACCCTCACTAAAGGG
            High quality sequence stop: 568.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Hordeum vulgare subsp. vulgare"
                     /mol_type="mRNA"
                     /cultivar="Morex"
                     /sub_species="vulgare"
                     /db_xref="taxon:112509"
                     /clone="HVSMEg0018J22f"
                     /tissue_type="pre-anthesis spike"
                     /clone_lib="SAMN00159197 Hordeum vulgare pre-anthesis
                     spike EST library HVcDNA0008 (white to yellow anther)"
                     /lab_host="SOLR"
                     /note="Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1;
                     Plants were grown in the greenhouse at the University of
                     California, Riverside (Fenton, SJ Close, TJ Close). Whole
                     spike with awns trimmed were collected at white, green and
                     yellow anther stages (Fenton). Total RNA was prepared from
                     each pool, equal quantities of all three RNA pools were
                     combined, poly(A) RNA was purified from the mixture, one
                     primary unamplified cDNA library was made, and 1 million
                     pfu were in vivo excised to give pBluescript SK(-) cDNA
                     phagemids. These steps were performed in the TJ Close lab
                     (Choi) at the University of California, Riverside.
                     Phagemids were plated and picked at the Clemson University
                     Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins
                     and Wing) Plasmid DNA preparations, DNA sequencing and
                     sequence analysis were performed at CUGI (Wing, Yu,
                     Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence
                     has been trimmed to remove vector sequence and contains a
                     minimum of 100 bases of phred value 20 or above. For more
                     details on library preparation and sequence analysis see
                     http://www.genome.clemson.edu/projects/barley. To order
                     this clone see http://www.genome.clemson.edu/orders Also
                     see Close TJ, Wing R, Kleinhofs A, Wise R (2001)
                     Genetically and physically anchored EST resources for
                     barley genomics. Barley Genetics Newsletter 31:29-30.
                     (http://wheat.pw.usda.gov/ggpages/bgn/31/cover.html)"
BASE COUNT          144 a          145 c          175 g          123 t
ORIGIN      
        1 gtcacacgaa ctctctcacg tcacacacgc ctcctcctcc tcctcctcct cctgcgagcc
       61 agcccagcca ctgggagcgc ccgcggaagc acagagcggg aggagggatc gcgagatgga
      121 ctgcaaggat gtcgggatcc tcgccatgga catgtacttc cctcccacct gcgtccagca
      181 ggaagcgctg gaggttcatg acggggccag caaggggaag tacacaattg gtcttgggca
      241 agactgcatg gccttctgca gcgaggtaga agatgtcatc tcgatgagtt tgacagttgt
      301 caaatccctg ctggaaaagt accacataga tccaaagcta attggccgcc tggaggtcgg
      361 tagcgagaca gtgatagaca aaagtaaatc catcaaaacg tggctgatgc aaatttttga
      421 ggaaagtggt aatactgaca ttgagggagt tgactcgagt aacgcatgtt atggtgggac
      481 agctgccctg ttgaactgtg tgaattgggt cgaaagtcga tgctgggatg gacgctacgg
      541 ccttgtggtc tgcacagata gcgcggttta tgcagaggga ccagctt
//