LOCUS BE425171 215 bp mRNA linear EST 24-JUL-2000 DEFINITION WHE0305_A01_A01ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0305_A01_A01, mRNA sequence. ACCESSION BE425171 VERSION BE425171.1 DBLINK BioSample: SAMN00160005 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 215) AUTHORS Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C. TITLE The structure and function of the expressed portion of the wheat genomes JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer. FEATURES Location/Qualifiers source 1..215 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Chinese Spring" /db_xref="taxon:4565" /clone="WHE0305_A01_A01" /tissue_type="Etiolated shoot" /clone_lib="SAMN00160005 Wheat unstressed seedling shoot cDNA library" /dev_stage="Five day old seedling" /lab_host="E. coli SOLR" /note="Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 27 a 94 c 77 g 17 t ORIGIN 1 agcaccaacc cattgcccgc cggcacacca ccacggcaat ggcgtcggcg tcgcggatcc 61 tgctcgtggc cgcgctgctc ctggcggcgt cggcgatgac gttggcgcag aaggcgaagg 121 cgcccgcgaa gccggccccc gccgccgccg acgcgcccgc ggccgtcgag gcgcccgcga 181 agcccgcccc ggccgcagcc gacgcgcccg gcgcg //