LOCUS       BE425171                 215 bp    mRNA    linear   EST 24-JUL-2000
DEFINITION  WHE0305_A01_A01ZS Wheat unstressed seedling shoot cDNA library
            Triticum aestivum cDNA clone WHE0305_A01_A01, mRNA sequence.
ACCESSION   BE425171
VERSION     BE425171.1
DBLINK      BioSample: SAMN00160005
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D.,
            Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J.,
            Seaton,C.L. and Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Stratagene SK primer.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE0305_A01_A01"
                     /tissue_type="Etiolated shoot"
                     /clone_lib="SAMN00160005 Wheat unstressed seedling shoot
                     cDNA library"
                     /dev_stage="Five day old seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Seeds were
                     surface-sterilized, germinated and grown aseptically in
                     the dark at room temperature on filter paper with water,
                     nystatin and cefotaxime in covered crystallization dishes.
                     Shoots were harvested. The tissue, total RNA, and poly(A)
                     RNA were prepared, a cDNA library was made, and the cDNA
                     clones were in vivo excised to give pBluescript phagemids
                     in the TJ Close lab (Choi, Close, Fenton) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT           27 a           94 c           77 g           17 t
ORIGIN      
        1 agcaccaacc cattgcccgc cggcacacca ccacggcaat ggcgtcggcg tcgcggatcc
       61 tgctcgtggc cgcgctgctc ctggcggcgt cggcgatgac gttggcgcag aaggcgaagg
      121 cgcccgcgaa gccggccccc gccgccgccg acgcgcccgc ggccgtcgag gcgcccgcga
      181 agcccgcccc ggccgcagcc gacgcgcccg gcgcg
//