LOCUS BE403955 426 bp mRNA linear EST 21-JUL-2000 DEFINITION WHE0415_C08_F15ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0415_C08_F15, mRNA sequence. ACCESSION BE403955 VERSION BE403955.1 DBLINK BioSample: SAMN00159966 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 426) AUTHORS Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C. TITLE The structure and function of the expressed portion of the wheat genomes JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Strategene SK primer. FEATURES Location/Qualifiers source 1..426 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Chinese Spring" /db_xref="taxon:4565" /clone="WHE0415_C08_F15" /tissue_type="Root" /clone_lib="SAMN00159966 Wheat etiolated seedling root cDNA library" /dev_stage="Five day old etiolated seedling" /lab_host="E. coli SOLR" /note="Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 100 a 106 c 123 g 97 t ORIGIN 1 ctcgacctgt tgctagcaga gacacagccc aaaaagactg ctcccaggtt ggtctgtgca 61 cctttgatgg gactcgtggt gttagtgctg gaattcggag cgtggttgat gatgagcctg 121 tgaaatcaaa tggcctccgt accgatccca tgtgcagtgc ctgtgagatg gctgttgtgt 181 ggatgcggag ccaacttgca cagaacaaga cccaggatct catactggat tacgttaacc 241 agctgtgcaa ccgtctcccg agccccatgg gagaatcagc cgtggactgt gccagcctcg 301 gatccatgcc tgacattgag ttcaccattg gcggcaagaa gtttgcgctg aaaccagaag 361 agtatattct gaaggttggt gaaggagccg ctgcccagtg catcagtgga ttcacagcca 421 tggaca //