LOCUS       BE403955                 426 bp    mRNA    linear   EST 21-JUL-2000
DEFINITION  WHE0415_C08_F15ZS Wheat etiolated seedling root cDNA library
            Triticum aestivum cDNA clone WHE0415_C08_F15, mRNA sequence.
ACCESSION   BE403955
VERSION     BE403955.1
DBLINK      BioSample: SAMN00159966
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D.,
            Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J.,
            Seaton,C.L. and Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Strategene SK primer.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE0415_C08_F15"
                     /tissue_type="Root"
                     /clone_lib="SAMN00159966 Wheat etiolated seedling root
                     cDNA library"
                     /dev_stage="Five day old etiolated seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Seeds were
                     surface-sterilized, germinated and grown aseptically in
                     the dark at room temperature on filter paper with water,
                     nystatin and cefotaxime in covered crystallization dishes.
                     Roots were harvested. The tissue, total RNA, and poly(A)
                     RNA were prepared, a cDNA library was made, and the cDNA
                     clones were in vivo excised to give pBluescript phagemids
                     in the TJ Close lab (Choi, Close, Fenton) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT          100 a          106 c          123 g           97 t
ORIGIN      
        1 ctcgacctgt tgctagcaga gacacagccc aaaaagactg ctcccaggtt ggtctgtgca
       61 cctttgatgg gactcgtggt gttagtgctg gaattcggag cgtggttgat gatgagcctg
      121 tgaaatcaaa tggcctccgt accgatccca tgtgcagtgc ctgtgagatg gctgttgtgt
      181 ggatgcggag ccaacttgca cagaacaaga cccaggatct catactggat tacgttaacc
      241 agctgtgcaa ccgtctcccg agccccatgg gagaatcagc cgtggactgt gccagcctcg
      301 gatccatgcc tgacattgag ttcaccattg gcggcaagaa gtttgcgctg aaaccagaag
      361 agtatattct gaaggttggt gaaggagccg ctgcccagtg catcagtgga ttcacagcca
      421 tggaca
//