LOCUS BD069514 5611 bp DNA linear PAT 24-AUG-2012
DEFINITION JP 2001511642-A/8: Improved BARSTAR gene.
ACCESSION BD069514
VERSION BD069514.1
KEYWORDS JP 2001511642-A/8.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 5611)
AUTHORS Michiels,F. and Williams,M.
TITLE Improved BARSTAR gene
JOURNAL Patent: JP 2001511642-A 8 14-AUG-2001;
PLANT GENETIC SYSTEMS NV
COMMENT OS Artificial Sequence
PN JP 2001511642-A/8
PD 14-AUG-2001
PF 01-SEP-1997 JP 1998512220
PR 03-SEP-1996 EP 96202446.9
PA PLANT GENETIC SYSTEMS NV
PI FRANK MICHIELS,MARK WILLIAMS
PT "Improved BARSTAR gene"
PS
PC C12N15/82,C12N15/31,C07K14/32,A01H5/00
CC Description of Artificial Sequence: other nucleic acid,
CC 'plasmid pLH48'
CC label = 3'nos, 'region containing 3' untranslated end of
CC nopaline synthase
CC gene of Agrobacterium T-DNA'
CC label = bar, 'region coding for phosphinothricin acetyl
CC transferase'
CC label = P35S, '35S promoter of Cauliflower Mosaic Virus'
CC label = PTA29, 'promoter of TA29 gene of Nicotiana tabacum'
CC label = synb*, 'improved barstar DNA'
CC label = 3'chs, 'region containing 3' untranslated end of
CC chalcone synthase
CC gene'
FH Key Location/Qualifiers
FT source 1..5611
FT /organism="Artificial Sequence"
FEATURES Location/Qualifiers
source 1..5611
/mol_type="unassigned DNA"
/db_xref="taxon:32630"
/organism="synthetic construct"
BASE COUNT 1389 a 1342 c 1353 g 1527 t
ORIGIN
1 agcttgcatg cctgcaggtc gactctagag gatcttcccg atctagtaac atagatgaca
61 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
121 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
181 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
241 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttcgg
301 atcctagacg cgtgagatca gatctcggtg acgggcagga ccggacgggg cggtaccggc
361 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc
421 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg
481 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc
541 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg
601 gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg
661 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg
721 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc
781 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg
841 atgtcggccg ggcgtcgttc tgggtccatg gttatagaga gagagataga tttatagaga
901 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
961 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atgtcacatc
1021 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
1081 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaatgata gcctttcctt
1141 tatcgcaatg atggcatttg taggagccac cttccttttc tactgtcctt tcgatgaagt
1201 gacagatagc tgggcaatgg aatccgagga ggtttcccga aattatcctt tgttgaaaag
1261 tctcaatagc cctttggtct tctgagactg tatctttgac atttttggag tagaccagag
1321 tgtcgtgctc caccatgttg acgaagattt tcttcttgtc attgagtcgt aaaagactct
1381 gtatgaactg ttcgccagtc ttcacggcga gttctgttag atcctcgatt tgaatcttag
1441 actccatgca tggccttaga ttcagtagga actacctttt tagagactcc aatctctatt
1501 acttgccttg gtttatgaag caagccttga atcgtccata ctggaatagt acttctgatc
1561 ttgagaaata tgtctttctc tgtgttcttg atgcaattag tcctgaatct tttgactgca
1621 tctttaacct tcttgggaag gtatttgatc tcctggagat tgttactcgg gtagatcgtc
1681 ttgatgagac ctgctgcgta ggagcttgca tgcctgcagg tcgactctag aggatcccca
1741 tctagctaag tataactgga taatttgcat taacagattg aatatagtgc caaacaagaa
1801 gggacaattg acttgtcact ttatgaaaga tgattcaaac atgatttttt atgtactaat
1861 atatacatcc tactcgaatt aaagcgacat aggctcgaag tatgcacatt tagcaatgta
1921 aattaaatca gtttttgaat caagctaaaa gcagacttgc ataaggtggg tggctggact
1981 agaataaaca tcttctctag cacagcttca taatgtaatt tccataactg aaatcagggt
2041 gagacaaaat tttggtactt tttcctcaca ctaagtccat gtttgcaaca aattaataca
2101 tgaaacctta atgttaccct cagattagcc tgctactccc cattttcctc gaaatgctcc
2161 aacaaaagtt agttttgcaa gttgttgtgt atgtcttgtg ctctatatat gcccttgtgg
2221 tgcaagtgta acagtacaac atcatcactc aaatcaaagt ttttacttaa agaaattagc
2281 taccatggcc aagaaggctg tcatcaacgg ggagcagatc aggagcatca gcgacctcca
2341 ccagaccctc aagaaggagc ttgcccttcc ggagtactac ggtgagaacc tcgacgccct
2401 ctgggactgc ctcaccggct gggtggagta ccccctcgtg ttggagtgga ggcagttcga
2461 gcagagcaag cagctcaccg agaacggcgc cgagagcgtg ctccaagtgt tcagggaggc
2521 caaggccgag ggctgcgaca tcaccatcat cctctcctga tggatctggg gccgctctag
2581 aactagtgga tcccccgggc tgcaggtcgg gttgggttat tttcttattt ccgtaataaa
2641 aaagtggaca tggttaccta ttattgtgat gtgtgctgca tgtgagctat attggcgatt
2701 tctctcttgt aacgctttgt acttgtaccg tttcgttgtg atcattgaaa taaaggccta
2761 tataaaaata atttatgtta tttgttggtt tatgtgtgtg tttttttttt ttttttttag
2821 tcaaaatatt taagtatttc tatataaatc ttttgcaagt tttttaagtc atggaggaga
2881 tgttatgaat tctgtaatat agtaaaaata ttacgtgaaa aaccagagga tccggggaat
2941 tcccagatcc gcctaccttt cacgagttgc gcagtttgtc tgcaagactc tatgagaagc
3001 agataagcga taagtttgct caacatcttc tcgggcataa gtcggacacc atggcatcac
3061 agtatcgtga tgacagaggc agggagtggg acaaaattga aatcaaataa tgattttatt
3121 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc
3181 aacttagtat aaaaaagcag gcttcatccg gattctctga gcccaccgtg ttcaccacca
3241 ccgtggtgct gttacgtctg gctttcagct gaatggtgca gttctgtacc ggttttcctg
3301 tgccgtcttt caggactcct gaaatcttta ctgccatatt caccccacaa aaaagcccac
3361 cggttccggc gggctgtcat aacactgtgt tacctggcta atcagaattt ataaccgacc
3421 ccaacgatga atccgtcagt acgccagtcg ccactgccgg agccttcata agcaatatca
3481 acaacgacgg acgctgccgg attaatctgt atacctgcac tccacgccac tgaggtatgc
3541 cgcattgcac tttcgtccct ggcagtggtc gtctctttca tatacccgac tctagaggat
3601 cccccgggta ccgagctcga attctgatca ggccaacgcg cggggagagg cggtttgcgt
3661 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
3721 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
3781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
3841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
3901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
3961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4021 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
4081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4261 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
4321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
4501 gggattttgg tcatgagact cgagccaaaa aggatcttca cctagatcct tttaaattaa
4561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
4621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
4681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
4741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
4801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
4861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
4921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
4981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
5041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
5101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
5401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
5461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
5521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
5581 acatttcccc gaaaagtgcc acctgacgtc a
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