LOCUS BC128156 1718 bp mRNA linear HUM 19-MAR-2009
DEFINITION Homo sapiens tRNA isopentenyltransferase 1, mRNA (cDNA clone
MGC:149243 IMAGE:40112698), complete cds.
ACCESSION BC128156
VERSION BC128156.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1718)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1718)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-DEC-2006) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Baylor Human Genome Sequencing Center
cDNA Library Preparation: Baylor Human Genome Sequencing Center
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAM Plate: 30 Row: d Column: 6
The stop codon of the CDS annotated on this record is located > 55
bases upstream of a splice junction, and therefore the mRNA is
predicted to be subject to nonsense-mediated mRNA decay (NMD).
FEATURES Location/Qualifiers
source 1..1718
/db_xref="H-InvDB:HIT000389684"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:149243 IMAGE:40112698"
/tissue_type="PCR rescued clones"
/clone_lib="NIH_MGC_282"
/note="Vector: pCR-Blunt II-TOPO; Clone identification
sequence tag: CGATCTC sequenced from the reverse primer"
gene 1..1718
/gene="TRIT1"
/gene_synonym="IPT"
/gene_synonym="MOD5"
/db_xref="GeneID:54802"
/db_xref="HGNC:HGNC:20286"
CDS 15..833
/gene="TRIT1"
/gene_synonym="IPT"
/gene_synonym="MOD5"
/codon_start=1
/product="TRIT1 protein"
/protein_id="AAI28157.1"
/db_xref="GeneID:54802"
/db_xref="HGNC:HGNC:20286"
/translation="MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLG
QRLGGEIVSADSMQVYEGLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRA
TALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGL
VLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGP
LKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENR"
BASE COUNT 480 a 391 c 436 g 411 t
ORIGIN
1 cagactgcca taagatggcg tccgtggcgg ctgcacgagc agttcctgtg ggcagtgggc
61 tcaggggcct gcaacggacc ctacctcttg tagtgattct cggggccacg ggcaccggca
121 aatccacgct ggcgttgcag ctaggccagc ggctcggcgg tgagatcgtc agcgctgact
181 ccatgcaggt ctatgaaggc ctagacatca tcaccaacaa ggtttctgcc caagagcaga
241 gaatctgccg gcaccacatg atcagctttg tggatcctct tgtgaccaat tacacagtgg
301 tggacttcag aaatagagca actgctctga ttgaagatat atttgcccga gacaaaattc
361 ctattgttgt gggaggaacc aattattaca ttgaatctct gctctggaaa gttcttgtca
421 ataccaagcc ccaggagatg ggcactgaga aagtgattga ccgaaaagtg gagcttgaaa
481 aggaggatgg tcttgtactt cacaaacgcc taagccaggt ggacccagaa atggctgcca
541 agctgcatcc acatgacaaa cgcaaagtgg ccaggagctt gcaagttttt gaagaaacag
601 gaatctctca tagtgaattt ctccatcgtc aacatacgga agaaggtggt ggtccccttg
661 gaggtcctct gaagttctct aacccttgca tcctttggct tcatgctgac caggcagttc
721 tagatgagcg cttggataag agggtggatg acatgcttgc tgctgggctc ttggaggaac
781 taagagattt tcacagacgc tataatcaga agaatgtttc ggaaaataga tgaaatggct
841 caactaggtc catctccaaa gcccctgcta gcttttagga gcaatggctt atctgctgat
901 accctcctgt tcccagccag gactatcaac atggtatctt ccaatcaatt ggcttcaagg
961 aatttcacga gtacctgatc actgagggaa aatgcacact ggagactagt aaccagcttc
1021 taaagaaagg tattgaggct ctgaaacaag taactaagag atatgcccgg aaacaaaacc
1081 gatgggttaa aaaccgtttt ttgagcagac ctggtcccat tgtcccccct gtctatggct
1141 tagaggtatc tgatgtctcg aagtgggaag agtctgttct tgaacctgct cttgaaatcg
1201 tgcaaagttt catccagggc cacaagccta cagccactcc aataaagatg ccatacaatg
1261 aagctgagaa caagagaagt tatcacctgt gtgacctctg tgatcgaatc atcattgggg
1321 atcgcgaatg ggcagcgcac ataaaatcca aatcccactt gaaccaactg aagaaaagaa
1381 gaagattgga ctcagatgct gtcaacacca tagaaagtca gagtgtttcc ccagaccata
1441 acaaagaacc taaagagaag ggatccccag ggcagaatga tcaagagctg aaatgcagcg
1501 tttaagagac atgtccagtg gcctttggaa aggtggtggg gatccagttc aggagggagg
1561 ggtatgtttg tctcccagtc tgggcaaagg agtgctatgc ggaattctct gcatagcaga
1621 aaagctccca ccattttctt ttgatgtggt tttaaagtct cacgttctct ataatagaaa
1681 cagcaggtct tgtcagctcc ttgtgtggct gatgtgtt
//