LOCUS BC063620 2627 bp mRNA linear HUM 02-JAN-2004
DEFINITION Homo sapiens agrin, mRNA (cDNA clone IMAGE:4179742), partial cds.
ACCESSION BC063620
VERSION BC063620.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2627)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2627)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (08-DEC-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: David N. Louis, M.D.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 134 Row: b Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2627
/db_xref="H-InvDB:HIT000261074"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4179742"
/tissue_type="Brain, anaplastic oligodendroglioma with
1p/19q loss"
/clone_lib="NCI_CGAP_Brn67"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2627
/gene="AGRN"
/gene_synonym="FLJ45064"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
CDS <1..1469
/gene="AGRN"
/gene_synonym="FLJ45064"
/codon_start=3
/product="AGRN protein"
/protein_id="AAH63620.1"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
/translation="CHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAA
PCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLG
EKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSRE
PVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLA
RAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCV
PREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSN
HFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNT
NRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPK
AYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP"
misc_feature 3..86
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="EGF_CA; Region: Calcium-binding EGF-like domain,
present in a large number of membrane-bound and
extracellular (mostly animal) proteins. Many of these
proteins require calcium for their biological function and
calcium-binding sites have been found to be located at the
N-terminus of particular EGF-like domains"
/db_xref="CDD:cd00054"
misc_feature 333..728
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
misc_feature 792..890
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="EGF_CA; Region: Calcium-binding EGF-like domain,
present in a large number of membrane-bound and
extracellular (mostly animal) proteins. Many of these
proteins require calcium for their biological function and
calcium-binding sites have been found to be located at the
N-terminus of particular EGF-like domains"
/db_xref="CDD:cd00054"
misc_feature 1023..1415
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
BASE COUNT 441 a 848 c 848 g 490 t
ORIGIN
1 gctgccatgg cggggcccca tgccagaacc tggaggctgg aaggttccat tgccagtgcc
61 cgcccggccg cgtcggacca acctgtgccg atgagaagag cccctgccag cccaacccct
121 gccatggggc ggcgccctgc cgtgtgctgc ccgagggtgg tgctcagtgc gagtgccccc
181 tggggcgtga gggcaccttc tgccagacag cctcggggca ggacggctct gggcccttcc
241 tggctgactt caacggcttc tcccacctgg agctgagagg cctgcacacc tttgcacggg
301 acctggggga gaagatggcg ctggaggtcg tgttcctggc acgaggcccc agcggcctcc
361 tgctctacaa cgggcagaag acggacggca agggggactt cgtgtcgctg gcactgcggg
421 accgccgcct ggagttccgc tacgacctgg gcaagggggc agcggtcatc aggagcaggg
481 agccagtcac cctgggagcc tggaccaggg tctcactgga gcgaaacggc cgcaagggtg
541 ccctgcgtgt gggcgacggc ccccgtgtgt tgggggagtc cccggttccg cacaccgtcc
601 tcaacctgaa ggagccgctc tacgtagggg gcgctcccga cttcagcaag ctggcccgtg
661 ctgctgccgt gtcctctggc ttcgacggtg ccatccagct ggtctccctc ggaggccgcc
721 agctgctgac cccggagcac gtgctgcggc aggtggacgt cacgtccttt gcaggtcacc
781 cctgcacccg ggcctcaggc cacccctgcc tcaatggggc ctcctgcgtc ccgagggagg
841 ctgcctatgt gtgcctgtgt cccgggggat tctcaggacc gcactgcgag aaggggctgg
901 tggagaagtc agcgggggac gtggatacct tggcctttga cgggcggacc tttgtcgagt
961 acctcaacgc tgtgaccgag agcgagaagg cactgcagag caaccacttt gaactgagcc
1021 tgcgcactga ggccacgcag gggctggtgc tctggagtgg caaggccacg gagcgggcag
1081 actatgtggc actggccatt gtggacgggc atctgcaact gagctacaac ctgggctccc
1141 agcccgtggt gctgcgttcc accgtgcccg tcaacaccaa ccgctggttg cgggtcgtgg
1201 cacataggga gcagagggaa ggttccctgc aggtgggcaa tgaggcccct gtgaccggct
1261 cctccccgct gggcgccacg cagctggaca ctgatggagc cctgtggctt gggggcctgc
1321 cggagctgcc cgtgggccca gcactgccca aggcctacgg cacaggcttt gtgggctgct
1381 tgcgggacgt ggtggtgggc cggcacccgc tgcacctgct ggaggacgcc gtcaccaagc
1441 cagagctgcg gccctgcccc accccatgag ctggcaccag agccccgtgc ccgctgtaat
1501 tattttctat ttttgtaaac ttgtcgcttt ttgatatgat tttcttgcct gagtgttggc
1561 cggagggact gctggcccgg cctcccttcc gtccaggcag ccgtgctgca gacagaccta
1621 gtgctgaggg atggacaggc gaggtggcag cgtggagggc tcggcgtgga tggcagcctc
1681 aggacacaca cccctgcctc aaggtgctga gcccccgcct tgcactgcgc ctgccccacg
1741 gtgtccccgc cgggaagcag ccccggctcc tgaatcaccc tcgctccgtc aggcgggact
1801 cgtgtcccag agaggaaggg gctgctgagg tctgatgggg cccttcctcc gggtgacccc
1861 acagggcctt tccaagcccc tatttgagct gctccttcct gtgtgtgctc tggaccctgc
1921 ctcggcctcc tgcgccaata ctgtgacttc caaacaatgt tactgctggg cacagctctg
1981 cgttgctccc gtgctgcctg cgccagcccc aggctgctga ggagcagagg ccagaccagg
2041 gccgatctgg gtgtcctgac cctcagctgg ccctgcccag ccaccctgga catgaccgta
2101 tccctctgcc acaccccagg ccctgcgagg ggctatcgag aggagctcac tgtgggatgg
2161 ggttgacctc tgccgcctgc ctgggtatct gggcctggcc atggctgtgt tcttcatgtg
2221 ttgattttat ttgacccctg gagtggtggg tctcatcttt cccatctcgc ctgagagcgg
2281 ctgagggctg cctcactgca aatcctcccc acagcgtcag tgaaagtcgt ccttgtctca
2341 gaatgaccag gggccagcca gtgtctgacc aaggtcaagg ggcaggtgca gaggtggcag
2401 ggatggctcc gaagccagaa atgccttaaa ctgcaacgtc ccgtcccttc cccaccccca
2461 tcccatcccc acccccagcc ccagcccagt cctcctagga gcaggacccg atgaagcggg
2521 cggcggtggg gctgggtgcc gtgttactaa ctctagtatg tttctgtgtc aatcgctgtg
2581 aaataaagtc tgaaaacttt aaaaaaaaaa aaaaaaaaaa aaaaaaa
//