LOCUS BC060776 3784 bp mRNA linear HUM 21-JUL-2005
DEFINITION Homo sapiens Rho guanine nucleotide exchange factor (GEF) 7, mRNA
(cDNA clone MGC:71542 IMAGE:5271977), complete cds.
ACCESSION BC060776
VERSION BC060776.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3784)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3784)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Sequencing Group at the Stanford Human Genome
Center, Stanford University School of Medicine, Stanford, CA 94305
Web site: http://www-shgc.stanford.edu
Contact: (Dickson, Mark) mcd@paxil.stanford.edu
Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
R. M.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 133 Row: n Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 22027527.
FEATURES Location/Qualifiers
source 1..3784
/db_xref="H-InvDB:HIT000260078"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:71542 IMAGE:5271977"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3784
/gene="ARHGEF7"
/gene_synonym="BETA-PIX"
/gene_synonym="COOL1"
/gene_synonym="DKFZp761K1021"
/gene_synonym="KIAA0142"
/gene_synonym="P50"
/gene_synonym="P50BP"
/gene_synonym="P85"
/gene_synonym="P85COOL1"
/gene_synonym="P85SPR"
/gene_synonym="PAK3"
/gene_synonym="PIXB"
/db_xref="GeneID:8874"
/db_xref="MIM:605477"
CDS 251..2662
/gene="ARHGEF7"
/gene_synonym="BETA-PIX"
/gene_synonym="COOL1"
/gene_synonym="DKFZp761K1021"
/gene_synonym="KIAA0142"
/gene_synonym="P50"
/gene_synonym="P50BP"
/gene_synonym="P85"
/gene_synonym="P85COOL1"
/gene_synonym="P85SPR"
/gene_synonym="PAK3"
/gene_synonym="PIXB"
/codon_start=1
/product="ARHGEF7 protein"
/protein_id="AAH60776.1"
/db_xref="GeneID:8874"
/db_xref="MIM:605477"
/translation="MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRL
LERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILL
SASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQ
SLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEE
GGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVL
QNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLE
ECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGAS
SPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQE
VRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVL
LMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDL
QEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLP
HPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKK
LLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLM
HNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSY
ISHQN"
BASE COUNT 955 a 997 c 956 g 876 t
ORIGIN
1 gcggccgagg ccggggggcg gcggcgggcg cccgcaggtt cccgagccgc tcctgagaag
61 gcgcctgaca gcgggccggg gcgcacggag aagcgggccg ggccggacct gctgggccgc
121 gccgagccaa tcgccggcgc cggccgctcg atgggcgagg cggcggcggc ggcggcgggg
181 gccgcgggcc gggccgccgc tccgaggtga aggcgcgcgc ccctccccgc ctgcctcccg
241 ggccgcagcg atgaattccg ccgagcaaac cgttacgtgg ctcatcactc tgggggtgct
301 ggagtcgccc aaaaaaacca tctcggaccc ggagggcttt ctgcaggcgt cgctgaagga
361 tggggtggtc ctctgcaggc tgctggagcg cctgctcccc gggaccatcg agaaagtcta
421 ccccgagccc cggagcgaga gcgagtgcct gagcaacatc cgcgagttcc tgcgcggctg
481 cggggcttcc ctgcggctgg agctcctgtt tcctccttcc cagcctcctc agcatctggt
541 aaccaccatt ctcttgtctg cttctacgtt tgatgcaaat gatttgtatc aggggcagaa
601 ttttaacaag gtcctcagtt ccttagtgac tctaaataaa gtaacagcag acatcgggct
661 ggggagtgac tccgtgtgtg cccggccctc gtctcaccgc ataaagtctt ttgactccct
721 tggatcacag tctttgcaca ctcggacttc aaaactgttc cagggccagt atcggagttt
781 ggacatgacc gataatagca acaatcaact ggtagtaaga gcaaagttta acttccagca
841 gaccaatgag gacgagcttt ccttctcaaa aggagacgtc atccatgtca cccgtgtgga
901 agagggaggc tggtgggagg gcacactcaa cggccggacc ggctggttcc ccagcaacta
961 cgtgcgcgag gtcaaggcca gcgagaagcc tgtgtctccc aaatcaggaa cactgaagag
1021 ccctcccaaa ggatttgata cgactgccat aaacaaaagc tattacaatg tggtgctaca
1081 gaatatttta gaaacagaaa atgaatattc taaagaactt cagactgtgc tttcaacgta
1141 cctacggcca ttgcagacca gtgagaagtt aagttcagca aacatttcat atttaatggg
1201 aaatctagaa gaaatatgtt ctttccagca aatgctcgta cagtctttag aagaatgcac
1261 caagttgccc gaagctcagc agagagtcgg aggctgcttt ttaaacctga tgccacagat
1321 gaaaaccctg tacctcacgt attgtgccaa tcacccttct gcagtgaatg tcctcacgga
1381 acacagtgag gagttggggg agttcatgga gaccaaaggt gccagcagcc ctgggattct
1441 cgtgctgacc acgggcctga gcaaaccctt catgcgcctg gataaatacc ctacgctgct
1501 caaagagctc gagagacaca tggaggatta tcatacagat agacaagata ttcaaaaatc
1561 catggctgcc ttcaaaaacc tttcagccca atgtcaagaa gtccggaaga ggaaagagct
1621 tgagctgcag atcctgacgg aagccatccg gaactgggag ggcgatgaca ttaaaactct
1681 gggcaacgtc acttacatgt cccaggtcct gattcagtgt gccggaagtg aggaaaagaa
1741 tgaaagatat cttctactct tcccaaatgt tttgctaatg ttgtctgcca gtcctaggat
1801 gagtggcttt atctatcagg gaaagcttcc aacgacagga atgacaatca caaagcttga
1861 ggacagtgaa aatcatagaa atgcatttga aatatcaggg agcatgattg agcggatatt
1921 agtgtcgtgc aacaaccagc aggatctgca ggaatgggtg gagcacctac agaagcaaac
1981 gaaggtcacg tctgtgggaa accccaccat aaagcctcat tcagtgccat ctcataccct
2041 cccctcccac ccggtcactc cgtccagcaa gcacgcagac agcaagcccg cgccgctgac
2101 gcccgcctac cacacgctgc cccacccctc ccaccacggc accccgcaca ccaccatcaa
2161 ctggggaccc ctggagcctc cgaaaacacc caagccctgg agcctgagct gcctgcggcc
2221 cgcgcctccc ctccggccct cagctgctct ctgctacaag gaggatctta gtaagagccc
2281 taagaccatg aaaaagctgc tgcccaagcg caaacctgaa cggaagcctt cagatgagga
2341 gttcgcgtcc cggaaaagca cagctgcttt ggaagaagat gctcagattc tgaaagtcat
2401 tgaagcttac tgcaccagcg ccaaaacaag gcaaacactc aattcaacat ggcaaggcac
2461 tgacctgatg cataatcacg tcttggctga tgatgaccaa ccaagcctag actccctggg
2521 gcgtcgcagt agcctttctc gtttggagcc ttcagacctc tcggaagact ctgactatga
2581 cagtatatgg acagcccata gttacagaat gggttctaca tctcgtaaga gctgttgctc
2641 atatatctct caccagaact aatgcacttc tgagcttttc ttaacaaatg cttgttgtat
2701 cgcagcctgc ttttcttaga tgttttcttg ctgttccttg tctcttttat gtgatatttt
2761 aacaactttt gagagtgttc ctgatatttc ccattgtact ttgtggaggc ttaactgccg
2821 atgcgagtat acattcgaat gactgactat tggagggagg tggtgtgttc acgtgagcct
2881 acatccgagg gtgctcttcg taaggggtag ttgttgtttt atagctccaa atggtttttt
2941 tattctcagt gtagcaactg tttgatttct ttctggatac agctgtatga acacttttct
3001 attttatact gaaatcacac aggtttgtga tctaagtgcc agaaccatgg aggagactct
3061 ggccttatct gtagggacct aaatttttta agtgctatga acactgatca agtagttgtg
3121 gagagatgag aagaatatat gcagcaaagg tgctgtgagc ccagttccaa cggctgtgtt
3181 ctggtaatcg tgcagaacag catgtgggct ctgggccttg gcaggtgcac agacgggcgg
3241 gcgtcacgtg tgttcagagc ttgtctccac ctcacggctc tgtgctcttc tgtgtgctgc
3301 gttgtggccg tgaagtggga gctctccttt ccaaaggcag agcttactgt catggctcag
3361 atgttcactc gcatcttcac cccaaacttc cctgaattgc agtgatttct gttataaggc
3421 acatagcaag cctgtaccac tctacagtaa aatctctaca ctaacagttt aaagcccgaa
3481 agctgaagca ttcagcctct gcaagtgtag actactggac tgtagtagag gcatttggaa
3541 tacttacaaa gtcaagtgct gtggcttcag ttcccccggc tccccacccc tgacatccat
3601 acttatgttg tctggaagag ccatggacac atcattattt tcagcaacca gctgcagtga
3661 aactccattt acagcaaccc catagtataa aaccacaatg cctcattgta atgggatatt
3721 gaagttcatt gaagaattac taggttgcca tgtggctcac ttatttagaa aaaaaaaaaa
3781 aaaa
//