LOCUS BC040183 4122 bp mRNA linear HUM 28-JUL-2005
DEFINITION Homo sapiens Rap guanine nucleotide exchange factor (GEF) 4, mRNA
(cDNA clone MGC:42189 IMAGE:4801092), complete cds.
ACCESSION BC040183
VERSION BC040183.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4122)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4122)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC040183.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 70 Row: k Column: 9.
FEATURES Location/Qualifiers
source 1..4122
/db_xref="H-InvDB:HIT000052340"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:42189 IMAGE:4801092"
/tissue_type="Brain, hypothalamus"
/clone_lib="NIH_MGC_96"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..4122
/gene="RAPGEF4"
/gene_synonym="CAMP-GEFII"
/gene_synonym="CGEF2"
/gene_synonym="EPAC2"
/db_xref="GeneID:11069"
/db_xref="MIM:606058"
CDS 380..2983
/gene="RAPGEF4"
/gene_synonym="CAMP-GEFII"
/gene_synonym="CGEF2"
/gene_synonym="EPAC2"
/codon_start=1
/product="RAPGEF4 protein"
/protein_id="AAH40183.1"
/db_xref="GeneID:11069"
/db_xref="MIM:606058"
/translation="MLYKKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVP
LRPANTITKVPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMM
QQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEE
KKECDEELQDTMLLLSQMGRDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLST
TVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDD
FGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLE
KVPAGNRASNQGNSQPQQKYTVMSGTPEKILERFLETIRLEATLNEATDSVLNDFIMM
HCVFMPNTQLCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQE
DDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVILQQFN
TGDERAQKRQPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLI
IVKMSSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTF
ELMSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFW
VVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEK
LPSKFKKFYAEFESLMDTSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFI
DNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLS
QMSHRLEPRRP"
BASE COUNT 1277 a 831 c 891 g 1123 t
ORIGIN
1 cttttaaacc aagcggaagc ctgcttcgat gacttggact tatgtctgca ccagggtctc
61 attcctgtgt cttcaagctt ttccatggag tggcaaaatg attcaaatcc agaaaggaag
121 ggagtgaagt tattaggagc taactaaagt taaaaaaaaa aaaaaaaaaa aaaaaaggaa
181 gtgttttctt attgccttag actgcctgag ctttcctgga gaaccatcag caccagtttc
241 ctttctgtgg actgagagcc actcagtagt agacacgaac agggaatgaa cggcaatgaa
301 ggtacatttt gctgatcaaa taaaggatag atgctcactg gatgtctgag aactttgaga
361 gaccgaaaaa gaaggacgta tgctctacaa gaaatatcga cagtatatgg caggacttct
421 ggctcctcct tatggtgtta tggaaacggg ctctaacaat gacaggattc ctgacaagga
481 gaacacacct ctcattgaac ctcacgttcc tcttcgtcct gctaacacca ttaccaaggt
541 cccttcagag aagatcctca gagctggaaa aattttacga aatgccattc tctctcgagc
601 acctcacatg ataagagata gaaaatacca cctaaagaca tacagacaat gctgtgtggg
661 aactgaactg gtggactgga tgatgcagca gacaccatgt gttcactccc ggactcaagc
721 tgttggcatg tggcaagtcc tgttagaaga tggtgttctc aaccacgtgg accaggagca
781 ccatttccaa gacaaatatt tattctatcg atttctggat gatgagcacg aggatgcccc
841 tttgcctact gaggaggaga agaaggagtg tgatgaggag ctccaggaca ccatgctgct
901 gctgtcacag atgggccgcg acgcccacat gaggatgatc cttcgcaaac cacctggcca
961 gaggactgtg gatgacctag agattatcta tgaggagctt cttcatatta aagccttatc
1021 ccatctttct accacagtga aacgagagtt agcaggtgtt ctcatttttg agtctcacgc
1081 caaaggaggg actgtgttgt ttaaccaggg ggaagaaggt acctcctggt acattattct
1141 aaaaggatca gtgaatgtag tcatttacgg caagggtgtg gtctgcaccc tgcatgaagg
1201 agatgacttc ggcaagttag cactagtgaa tgatgcccca cgagctgcct ctatcgtctt
1261 acgagaagat aactgccatt tcttaagagt agacaaggag gatttcaacc ggatcctaag
1321 ggacgtggag gcgaatacag tcagacttaa agaacatgac caagatgtct tggtgctgga
1381 gaaggtccca gcagggaaca gagcttctaa tcaaggaaac tcacagcctc agcaaaagta
1441 tactgtgatg tcaggaacac ctgaaaaaat tttagagcgt tttctagaaa caatacgcct
1501 tgaggcaact ttaaatgaag caacagattc tgttttaaat gactttatta tgatgcactg
1561 tgtttttatg ccaaataccc agctttgccc ggcactggtg gcccactacc acgcacagcc
1621 ttcacaaggt acagaacagg agaaaatgga ttatgccctc aacaataaga ggcgagtcat
1681 ccgcctggtt ctacagtggg ctgccatgta tggagacctc ctgcaagagg atgacgtgtc
1741 tatggccttc ctggaggagt tttatgtatc tgtatcagat gatgcccgga tgattgctgc
1801 cctcaaggag caactgccag agttggagaa gattgtcaag caaatctcag aagatgcaaa
1861 ggcaccacaa aagaagcaca aggttatttt gcaacagttc aatacgggcg atgagagagc
1921 ccagaagcgc cagcctatcc gcggctctga tgaagttctg tttaaggtct attgcatgga
1981 ccacacctac acaaccattc gggtgccagt ggccacttcg gtgaaggaag tcatcagtgc
2041 agttgccgac aagctgggct ccggggaggg cctgatcata gtcaagatga gttccggagg
2101 agaaaaggtg gtgctcaaac ctaatgatgt ttcagtattt acgacgctca ccattaatgg
2161 acgcctgttt gcttgcccgc gagagcaatt cgattcactg actccattac cagaacagga
2221 aggcccaact gttggaacag tgggaacttt tgaactgatg agctccaaag atttagcata
2281 ccagatgaca atttatgatt gggaactctt caactgcgtg catgagctgg agctaatcta
2341 tcacacattt ggaaggcata attttaaaaa gaccacagca aacttggatt tgttcctgag
2401 gagatttaat gaaattcagt tttgggtcgt cactgagatc tgcctttgtt ctcagctcag
2461 caagcgtgtt cagctattaa aaaaatttat taagatagca gcccactgta aggagtataa
2521 aaatctgaat tccttttttg ccatcgtcat gggactaagt aacgttgctg tgagccgctt
2581 ggcactaacg tgggagaaac tgccaagcaa gttcaagaag ttctatgcgg agtttgaaag
2641 tttaatggac acttcaagga accacagggc ctacaggctg acagtagcta agctggaacc
2701 tcctctcatc cccttcatgc ctttgctcat taaagatatg acatttactc atgaggggaa
2761 caagacgttc attgacaatc tagtaaactt tgaaaaaatg cgcatgattg caaatacggc
2821 cagaacagtg agatactaca ggagccaacc cttcaatcct gatgcagctc aagctaataa
2881 gaaccatcag gatgtccgga gttatgtacg gcaattaaat gtgattgaca accagagaac
2941 tttatcacag atgtcacaca gattagagcc tcgtcgacca tagacatttc aaatgcccaa
3001 agcaacagtt tgtctccagt ccacaatctt tcaaaaatgc catttatgct actactgact
3061 gtattgccac tagagaattc tacaaaacaa gcaaaaacac atcctgagac acctcagggc
3121 tgcattcagc ttaccagcta cctagcaaga gaaggaatta tttgacatgc ctaaagctta
3181 cacactctag cttaggaatc cccagtttgt ggctaccctg tttcatttcc acttgtgcca
3241 tcttctttgc tgaagtgttg aataaggccc acgtgaagag tttggtagaa atagccttgt
3301 caagagaact agcaagcccc tgacattttt ttctaagagt gtttatggga tgagatgtgc
3361 tacaaatggg atagatttgg ataaaatttt aactcaacat cttgatttgg agcctggggt
3421 ttcgggaagg tgttgaggaa tctatagaag tccaaattat aggttgtatt cttccatctt
3481 cattgtctta cctctgaagg aatagaacct gaccacatta ttatgaaaca ttatggctgt
3541 ttatgtaatt tagggaggac tggtctgtaa tttctgaatg tatatagaat aatatttatg
3601 tttacaatgt aacttttcaa aatttacaaa tcaggattat atacataaga attccactaa
3661 gaaatgccaa gattcttaaa tttgccagcg ttaaagtaga aaataacatt tcagaagagc
3721 acaatatgca taaaacattt ttcaaaattg aaatattttc ctgggcatta aaaaccttta
3781 ctattggcta caaatttatt gtacctgatg aaaacatatt ttctggactt aaatgttatt
3841 acaaatatct taattttcag taattgtttt gcactttcaa agattgtaaa tagttcattc
3901 aatcaatggt atagagttat ttatttgcta cataatagat actgtgccaa ataattactt
3961 tttatttatt ttatttagta cgtaattttg aagtacattt tttcctgttt tcgcaattag
4021 actacattta atgtgtagga attgtatgta tgtatatctt ctgtaaataa catctagtat
4081 cttcactaaa tataattgtc gacaagaaaa aaaaaaaaaa aa
//