LOCUS BC040061 3160 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens protein kinase N1, mRNA (cDNA clone MGC:46204
IMAGE:5752583), complete cds.
ACCESSION BC040061
VERSION BC040061.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3160)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3160)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (22-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Life Technologies, Inc.
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 79 Row: n Column: 21.
FEATURES Location/Qualifiers
source 1..3160
/db_xref="H-InvDB:HIT000052313"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:46204 IMAGE:5752583"
/tissue_type="Brain, Lung, Testis, adult, pooled whole"
/clone_lib="NIH_MGC_115"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3160
/gene="PKN1"
/gene_synonym="DBK"
/gene_synonym="MGC46204"
/gene_synonym="PAK1"
/gene_synonym="PKN"
/gene_synonym="PRK1"
/db_xref="GeneID:5585"
/db_xref="HGNC:HGNC:9405"
/db_xref="MIM:601032"
CDS 166..2994
/gene="PKN1"
/gene_synonym="DBK"
/gene_synonym="MGC46204"
/gene_synonym="PAK1"
/gene_synonym="PKN"
/gene_synonym="PRK1"
/codon_start=1
/product="protein kinase N1"
/protein_id="AAH40061.1"
/db_xref="GeneID:5585"
/db_xref="HGNC:HGNC:9405"
/db_xref="MIM:601032"
/translation="MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRR
EIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPD
PAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDR
KLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEEL
RHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLG
ELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCR
DLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVS
TVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLE
DFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQM
NIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKS
SRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRG
HFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLF
GCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRD
LKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDW
WGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPER
RLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDISNFDEEFTGEAPT
LSPPRDARPLTAAEQAAFLDFDFVAGGC"
BASE COUNT 662 a 989 c 1015 g 494 t
ORIGIN
1 cggcggcctg gccaggcgag gcgggagccg agcaggccgg agcgggccgc gcgccgggga
61 gggtcgcgcc gcgcccgccc cctccccgcc acgcccgggg cgcacgcgcg ctcctctggc
121 cgcccctccc tccgcgcggg gacccctggc ggacggcagg aggacatggc cagcgacgcc
181 gtgcagagtg agcctcgcag ctggtccctg ctagagcagc tgggcctggc cggggcagac
241 ctggcggccc ccggggtaca gcagcagctg gagctggagc gggagcggct gcggcgggaa
301 atccgcaagg agctgaagct gaaggagggt gctgagaacc tgcggcgggc caccactgac
361 ctgggccgca gcctgggccc cgtagagctg ctgctgcggg gctcctcgcg ccgcctcgac
421 ctgctgcacc agcagctgca ggagctgcac gcccacgtgg tgcttcccga cccggcggcc
481 acccacgatg gcccccagtc ccctggtgcg ggtggcccca cctgctcggc caccaacctg
541 agccgcgtgg cgggcctgga gaagcagttg gccattgagc tgaaggtgaa gcagggggcg
601 gagaacatga tccagaccta cagcaatggc agcaccaagg accggaagct gctgctgaca
661 gcccagcaga tgttgcagga cagtaagacc aagattgaca tcatccgcat gcaactccgc
721 cgggcgctgc aggccggcca gctggagaac caggcagccc cggatgacac ccaagggagt
781 cctgacctgg gggctgtgga gctgcgcatc gaagagctgc ggcaccactt ccgagtggag
841 cacgcggtgg ccgagggtgc caagaacgta ctgcgcctgc tcagcgctgc caaggccccg
901 gaccgcaagg cagtcagcga ggcccaggag aaattgacag aatccaacca gaagctgggg
961 ctgctgcggg aggctctgga gcggagactt ggggagctgc ccgccgacca ccccaagggg
1021 cggctgctgc gagaagagct cgctgcggcc tcctccgctg ccttcagcac ccgcctggcc
1081 gggccctttc ccgccacgca ctacagcacc ctgtgcaagc ccgcgccgct cacagggacc
1141 ctggaggtac gagtggtggg ctgcagagac ctcccagaga ccatcccgtg gaaccctacc
1201 ccctcaatgg ggggacctgg gaccccagac agccgccccc ccttcctgag ccgcccagcc
1261 cggggccttt acagccgaag cggaagcctc agtggccgga gcagcctcaa agcagaagcc
1321 gagaacacca gtgaagtcag cactgtgctt aagctggata acacagtggt ggggcagacg
1381 tcttggaagc catgtggccc caatgcctgg gaccagagct tcactctgga gctggaaagg
1441 gcacgggaac tggagttggc tgtgttctgg cgggaccagc ggggcctgtg tgccctcaaa
1501 ttcctgaagt tggaggattt cttggacaat gagaggcatg aggtgcagct ggacatggaa
1561 ccccagggct gcctggtggc tgaggtcacc ttccgcaacc ctgtcattga gaggattcct
1621 cggctccgac ggcagaagaa aattttctcc aagcagcaag ggaaggcgtt ccagcgtgct
1681 aggcagatga acatcgatgt cgccacgtgg gtgcggctgc tccggaggct catccccaat
1741 gccacgggca caggcacctt tagccctggg gcttctccag gatccgaggc ccggaccacg
1801 ggtgacatat cggtggagaa gctgaacctc ggcactgact cggacagctc acctcagaag
1861 agctcgcggg atcctccttc cagcccatcg agcctgagct cccccatcca ggaatccact
1921 gctcccgagc tgccttcgga gacccaggag accccaggcc ccgccctgtg cagccctctg
1981 aggaagtcac ctctgaccct cgaagatttc aagttcctgg cggtgctggg ccggggtcat
2041 tttgggaagg tgctcctctc cgaattccgg cccagtgggg agctgttcgc catcaaggct
2101 ctgaagaaag gggacattgt ggcccgagac gaggtggaga gcctgatgtg tgagaagcgg
2161 atattggcgg cagtgaccag tgcgggacac cccttcctgg tgaacctctt cggctgtttc
2221 cagacaccgg agcacgtgtg cttcgtgatg gagtactcgg ccggtgggga cctgatgctg
2281 cacatccaca gcgacgtgtt ctctgagccc cgtgccatct tttattccgc ctgcgtggtg
2341 ctgggcctac agtttcttca cgaacacaag atcgtctaca gggacctgaa gttggacaat
2401 ttgctcctgg acaccgaggg ctacgtcaag atcgcagact ttggcctctg caaggagggt
2461 atgggctatg gggaccggac cagcacattc tgtgggaccc cggagttcct ggcccctgag
2521 gtgctgacgg acacgtcgta cacgcgagct gtggactggt ggggactggg tgtgctgctc
2581 tacgagatgc tggttggcga gtccccattc ccaggggatg atgaggagga ggtcttcgac
2641 agcatcgtca acgacgaggt tcgctacccc cgcttcctgt cggccgaagc catcggcatc
2701 atgagaaggc tgcttcggag gaacccagag cggaggctgg gatctagcga gagagatgca
2761 gaagatgtga agaaacagcc cttcttcagg actctgggct gggaagccct gttggcccgg
2821 cgcctgccac cgccctttgt gcccacgctg tccggccgca ccgacatcag caacttcgac
2881 gaggagttca ccggggaggc ccccacactg agcccgcccc gcgacgcgcg gcccctcaca
2941 gccgcggagc aggcagcctt cctggacttc gacttcgtgg ccgggggctg ctagccccct
3001 cccctgcccc tgcccctgcc cctgcccgag agctcttagt ttttaaaaag gcctttggga
3061 tttgccggaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//