LOCUS BC037310 3983 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens solute carrier family 1 (glial high affinity glutamate
transporter), member 3, mRNA (cDNA clone MGC:41861 IMAGE:5264000),
complete cds.
ACCESSION BC037310
VERSION BC037310.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3983)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3983)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC037310.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 73 Row: i Column: 17
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34222301.
FEATURES Location/Qualifiers
source 1..3983
/db_xref="H-InvDB:HIT000051903"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:41861 IMAGE:5264000"
/tissue_type="Brain, hippocampus"
/clone_lib="NIH_MGC_95"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3983
/gene="SLC1A3"
/gene_synonym="EA6"
/gene_synonym="EAAT1"
/gene_synonym="GLAST"
/gene_synonym="GLAST1"
/db_xref="GeneID:6507"
/db_xref="HGNC:HGNC:10941"
/db_xref="MIM:600111"
CDS 245..1873
/gene="SLC1A3"
/gene_synonym="EA6"
/gene_synonym="EAAT1"
/gene_synonym="GLAST"
/gene_synonym="GLAST1"
/codon_start=1
/product="solute carrier family 1 (glial high affinity
glutamate transporter), member 3"
/protein_id="AAH37310.1"
/db_xref="GeneID:6507"
/db_xref="HGNC:HGNC:10941"
/db_xref="MIM:600111"
/translation="MTKSNGEEPKMGGRMERFQQGVSKRTLLAKKKVQNITKEDVKSY
LFRNAFVLLTVTAVIVGTILGFTLRPYRMSYREVKYFSFPGELLMRMLQMLVLPLIIS
SLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMHREGK
IVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVS
EAMETLTRITEELVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNE
AIMRLVAVIMWYAPVGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLP
LLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLP
VGATINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVT
MVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVE
MGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM"
BASE COUNT 1195 a 835 c 879 g 1074 t
ORIGIN
1 ggtagtaact tgcagtttca gagcacatgc acactgtcag ggctagcctg cctgcttacg
61 cgcgctgcgg attgttgctc cgttgtacct gctggggaat tcacctcgtt actgctcgat
121 atcttccacc ccttacaaaa tcagaaaagt tgtgttttct aataccaaag aggaggtttg
181 gctttctgtg ggtgattccc agacactgaa gtgcaaagaa gagaccctcc tagaaaagta
241 aaatatgact aaaagcaatg gagaagagcc caagatgggg ggcaggatgg agagattcca
301 gcagggagtc agtaaacgca cacttttggc caagaagaaa gtgcagaaca ttacaaagga
361 ggatgttaaa agttacctgt ttcggaatgc ttttgtgctg ctcacagtca ccgctgtcat
421 tgtgggtaca atccttggat ttaccctccg accatacaga atgagctacc gggaagtcaa
481 gtacttctcc tttcctgggg aacttctgat gaggatgtta cagatgctgg tcttaccact
541 tatcatctcc agtcttgtca caggaatggc ggcgctagat agtaaggcat cagggaagat
601 gggaatgcga gctgtagtct attatatgac taccaccatc attgctgtgg tgattggcat
661 aatcattgtc atcatcatcc atcctgggaa gggcacaaag gaaaacatgc acagagaagg
721 caaaattgta cgagtgacag ctgcagatgc cttcctggac ttgatcagga acatgttccc
781 tccaaatctg gtagaagcct gctttaaaca gtttaaaacc aactatgaga agagaagctt
841 taaagtgccc atccaggcca acgaaacgct tgtgggtgct gtgataaaca atgtgtctga
901 ggccatggag actcttaccc gaatcacaga ggagctggtc ccagttccag gatctgtgaa
961 tggagtcaat gccctgggtc tagttgtctt ctccatgtgc ttcggttttg tgattggaaa
1021 catgaaggaa caggggcagg ccctgagaga gttctttgat tctcttaacg aagccatcat
1081 gagactggta gcagtaataa tgtggtatgc ccccgtgggt attctcttcc tgattgctgg
1141 gaagattgtg gagatggaag acatgggtgt gattgggggg cagcttgcca tgtacaccgt
1201 gactgtcatt gttggcttac tcattcacgc agtcatcgtc ttgccactcc tctacttctt
1261 ggtaacacgg aaaaaccctt gggtttttat tggagggttg ctgcaagcac tcatcaccgc
1321 tctggggacc tcttcaagtt ctgccaccct acccatcacc ttcaagtgcc tggaagagaa
1381 caatggcgtg gacaagcgcg tcaccagatt cgtgctcccc gtaggagcca ccattaacat
1441 ggatgggact gccctctatg aggctttggc tgccattttc attgctcaag ttaacaactt
1501 tgaactgaac ttcggacaaa ttattacaat cagcatcaca gccacagctg ccagtattgg
1561 ggcagctgga attcctcagg cgggcctggt cactatggtc attgtgctga catctgtcgg
1621 cctgcccact gacgacatca cgctcatcat cgcggtggac tggttcctgg atcgcctccg
1681 gaccaccaca aacgtactgg gagactccct gggagctggg attgtggagc acttgtcacg
1741 acatgaactg aagaacagag atgttgaaat gggtaactca gtgattgaag agaatgaaat
1801 gaagaaacca tatcaactga ttgcacagga caatgaaact gagaaaccca tcgacagtga
1861 aaccaagatg tagactaaca taaagaaaca ctttcttgag caccaggtgt taaaaaccat
1921 tataaaatct ttccatctca ttacagctca ttcgctccag caagcccgtc atcttccctt
1981 tcctcccttc tgataagact ggaaaatagt cctccaaaac acaagggagg attttgggtg
2041 gccaaagtgt acaattttca tcccacaatt gaaattttta aatcatttca tgttagtctt
2101 accgaataag gtaccaagat cacaaatagt gttgatcaga tcttacaagt ttatgtggca
2161 cacaatccta taaatgtgat ttttttatat aagttaaaga gacaaatagt aggctaaaaa
2221 cattttaaaa tcaacttttg aaatttaaaa atctttcaga atacaattca gttttagttt
2281 caaaatgtta acaacttgaa ttacaaccgg ttatcagttg gacagtaaga ttttatccct
2341 ttctcttctg actggtatac ctatttcatt agtagctagg tgcacatata catctagcac
2401 agctgtgagg acagacagaa ggcaaagttt ccatgtggcc ttgagcaagt cccatctcac
2461 ctctaggcct cagtgtcctc atctataaaa tgagggactt ccctagaagt cttcatggtc
2521 tcttccagcc cagacatcct gtgatgtcat gaaagcacct gccctctgtt tcccctcaga
2581 acaccctgta ccatccatgg agcacgaggc cttcagaaaa gacacttcaa tgggagtgaa
2641 catttctaac taaggacagg atggctgtgt gtggtggtca ccaggtcctg tgagcaaagt
2701 gcaggttatg caagtcgcca ggcaggaggc cattccagga gtgggattat tcatcaaact
2761 ctttgcccag ttcatcccaa tgggggaagt attcccttct ttcctactct gggaagaatg
2821 tctcctgcca ctcctcaact gatgatagac ttcgaaaaca gatgagaaga ctagcagcta
2881 gcaagggtgc ttgtagtcac actgtggaac actaaagagc taggaaagag ttgagcacag
2941 gcaacattac aaacaaagga tttgaaaaca ccaagagtac aggtcttctt taaggaagaa
3001 taaaaaagaa gaggttcatt tttctggctt tttttttcac ctgaaacact ttttctcgag
3061 tccaaaatca ttccccccgt gaagtctgct taccaaaaca taagacgact tatatatttg
3121 aaagaagtca aatgaatgag ctctctaata gaagtccatg agttgagtgg gtatttctta
3181 tttgaaagtg tttttcttta atcaaaagtc cttagaatga gggaaacaaa atatttattt
3241 gttttggaat cccacttatc aaatcattca aaactttcag ctggagtggg gtttgctttt
3301 gttttgtttg tgtccataag agaaatggta gaagatgaat cagtatgaag acactgtcaa
3361 tgaggttatg agaaaaacag caggggcatt agtttcaggc aaggcagctc ccaggtttag
3421 agattaattt ttacccccta aggaatatcc agtcaaagac gctgagtggg agctgtcagg
3481 cagtagcagc tgtgtttgag tttctggctg aaaatggtga agaatggact taatcatgct
3541 aacaaactga aaaatctaga catagatcct ctgatataca attagagata tttttatata
3601 gaccccaagc attctgtgca taaaagttaa cattaggctg tggtgcagta accatttaat
3661 gtcgaggctc tatttcggaa atacactaca aatgttaaag tacgtggctg tcctcttaag
3721 acactagtag agcaaagact taatcatatc aacttaattc tgttacacaa tatgtgtttt
3781 tttaatatac taaccatttc ttatggaaag gtcctgtggg gagcccatca tctcgccaag
3841 ccatcacagg ctctgcatac acatgcactc agtgtggact gggaagcatt actttgtaga
3901 tgtattttca ataaagaaaa aaatagtttt acattaaaaa aaaaaaaaaa aaaaaaaaaa
3961 aaaaaagaaa aaaaaaaaaa aaa
//