LOCUS BC035711 3778 bp mRNA linear HUM 15-JUL-2006 DEFINITION Homo sapiens N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2, mRNA (cDNA clone MGC:46209 IMAGE:5587622), complete cds. ACCESSION BC035711 VERSION BC035711.1 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 3778) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 3778) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (31-JUL-2002) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Invitrogen cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 79 Row: e Column: 2 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 31377809. FEATURES Location/Qualifiers source 1..3778 /db_xref="H-InvDB:HIT000051481" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46209 IMAGE:5587622" /tissue_type="Ovary, pooled from 3 adults" /clone_lib="NIH_MGC_125" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 1..3778 /gene="NDST2" /gene_synonym="HSST2" /gene_synonym="NCST2" /gene_synonym="NST2" /db_xref="GeneID:8509" /db_xref="HGNC:HGNC:7681" /db_xref="MIM:603268" CDS 558..3209 /gene="NDST2" /gene_synonym="HSST2" /gene_synonym="NCST2" /gene_synonym="NST2" /codon_start=1 /product="N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2" /protein_id="AAH35711.1" /db_xref="GeneID:8509" /db_xref="HGNC:HGNC:7681" /db_xref="MIM:603268" /translation="MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKA KEPLPLPLGDCSSGGAAGPGPARPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEI VAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRY CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLE PGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQ RVLFGHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADV EALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSH MQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVW GIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSI RGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLA QKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLS LHPAVTSSFPSPSTFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDS EVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQTP LALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQELRTNPAASMESIQKFLGIT PFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMDTESRLFLTDFFRNHNL ELSKLLSRLGQPVPSWLREELQHSSLG" BASE COUNT 813 a 1121 c 975 g 869 t ORIGIN 1 gagatggcgg agcagccgcg acgcggcccg tggccccgaa agcagggaag ccgggcagcc 61 ccgggacgcg gcggagcccg gggacagcgg ggacgccgcc cagtcctccg ggtatgaagc 121 taaaatgcag cggctaagaa cgcggctctg gagcttgaca ggcctgacgt cgaatcccag 181 ccctcccacc aactgagctg tgtgatcctg ggcaaggtag aggggaagag attgaacttt 241 gctgaccttt gatgtgaggc gctcagccag ggccaagggg agagcctggc aagatttgca 301 gcctgaagcc atgggccagg gggccatggt gacctgagac aagtggactc tgtatagttg 361 ccccctgctt ccccttctac ctcccctacc ctatgctaag gggactcgtc tccacctcgt 421 aaaggaaact ccccaaggga atccctgtcc cctattttcc tatccttcta cccttccaag 481 acagtcctag cctatagaac tcctacctcc catcccctga ggtggtcccc attcctccct 541 cccttcctcc ccccgccatg ctccagttgt ggaaggtggt acgcccagct cggcagctgg 601 aactgcaccg cctcatactg ctgctgatcg ctttcagcct gggctccatg ggcttcctgg 661 cttattatgt gtccaccagc cctaaggcca aggaaccctt gcccctgccc ttgggagact 721 gcagcagcgg tggggcagct ggtcctggcc ctgcacggcc tccagttcca cctcggcccc 781 ccaggcctcc agagacagct cgaactgaac ccgtggtcct tgtgtttgtg gagagtgcat 841 actcacagct ggggcaggaa attgtggcca tcctggagtc tagtcgtttt cgttatagca 901 ctgagttggc acctggccga ggggacatgc ccacattgac tgataatacc catggccgct 961 atgtcttggt catttatgag aacctgctca agtatgtcaa cctggatgcc tggagtcggg 1021 aactgctaga ccggtactgc gtggagtatg gtgtgggcat cattggcttt ttccgagccc 1081 acgagcacag cctactgagc gcccagctca agggctttcc ccttttttta cactcaaact 1141 tggggctccg ggactaccaa gtgaatcctt ctgccccgct actgcatctc acacgcccca 1201 gccgcctaga accagggcca ctgcctggtg atgactggac catcttccaa tccaatcata 1261 gtacatatga accagtgctt cttgccagcc ttcggccagc tgagcccgca gtgccaggac 1321 cagttcttcg tcgggcccgg cttcccactg tggtacagga cctggggctt catgatggca 1381 tccagcgggt gctctttgga catggccttt ccttctggct ccacaaactt atcttcgttg 1441 atgctgttgc atacctcact ggcaagcgcc tctgcctgga ccttgaccgc tacatcttgg 1501 tagacatcga tgacatcttt gtgggcaagg aagggacccg catgaaggtg gctgatgttg 1561 aggctctgtt gaccacccag aacaaactca ggaccttagt tcccaacttc accttcaact 1621 tgggcttctc gggcaagttc tatcatactg ggacagagga ggaggatgca ggggacgaca 1681 tgctgctgaa gcaccgcaaa gagttctggt ggttccccca catgtggagc cacatgcagc 1741 cacacctgtt ccacaatcgc tccgtgctgg ctgaccagat gaggctcaac aaacagtttg 1801 ctctggagca tgggattccc acggacctgg ggtatgctgt ggccccccac cactcgggtg 1861 tgtaccccat ccacacgcag ctctatgagg cctggaaatc cgtgtggggc atccaggtga 1921 ccagcactga ggagtatccc catctccgcc ctgcccgcta ccgccgtggc ttcattcaca 1981 atggcattat ggtgctgccc cggcagacat gtggcctctt cactcacaca atcttctata 2041 atgagtatcc tggaggctct cgtgaactag accggagcat ccgaggtgga gagctctttc 2101 tgacagtgct gcttaatccg atcagcatct ttatgaccca tctgtccaat tatggaaatg 2161 accggctggg cctatacacc tttgagagct tggtgcgctt cctccagtgt tggacacggc 2221 tgcgcctaca gacccttcct cctgtcccac ttgcacagaa gtactttgaa cttttccctc 2281 aggagcgaag ccccctttgg cagaatccct gtgatgacaa gaggcacaaa gatatctggt 2341 ccaaggagaa aacctgtgat cgtctcccga agttcctcat tgtgggaccc cagaaaacag 2401 ggactacagc tattcacttc ttcctgagcc tgcacccagc tgtaactagc agcttcccta 2461 gccccagcac atttgaggag attcagttct tcaacagccc taattaccac aagggtattg 2521 actggtacat ggatttcttc cctgttcctt ccaatgccag cactgatttc ctatttgaaa 2581 aaagtgccac ctactttgac tctgaagttg taccacggcg gggggctgcc ctcctgccac 2641 gagccaagat catcacagtg ctcaccaacc ctgctgacag ggcctactcc tggtaccagc 2701 atcagcgagc ccatggagac ccagttgctc tgaactatac cttctatcag gtgatttcag 2761 cctcctccca gacccctctg gcactacgct ccctgcagaa ccgctgtctt gtccctggct 2821 actattctac ccatctacaa cgctggctga cttactaccc ctctggacag ttgctgattg 2881 tggatgggca agagctgcgt accaacccag cagcctcaat ggagagcatc cagaagttcc 2941 tgggtatcac accctttctg aactacacac ggaccctcag gtttgatgat gataagggat 3001 tttggtgcca gggacttgaa ggtggtaaga ctcgctgtct aggccggagc aaaggccgga 3061 ggtatccaga tatggacact gagtcccgtc ttttccttac ggattttttc cggaaccata 3121 atttggagtt gtcgaagctg ctgagccggc ttggacagcc agtgccctcg tggcttcggg 3181 aagaactgca gcattccagt ctgggctgat gtcccagcct cccataccag caaaatgccc 3241 cctgcttccc taaggggcag gtccagagca gggcccacaa gggggattag agtggcctgg 3301 cccctccccc tctacctcag tagcccccag gcctgagatg gctgagaagg gaagggtatc 3361 cttttcccac agttctggga caaataaagg ggcttccttt ggtaccccac ataatagtgc 3421 taggtacctt tgacccatca tcttgggagg tggggaggaa tgagagggtc caggcagggt 3481 gtaggggaat gtattagtcc aatgagattt ccctcttcat ccgcagcagt gtatctattc 3541 tatacctggc tatgggagag accccttgca tgggagggac cccttgctat ggccccttta 3601 gccaggcagt gggatctacc tgtggcccgg cctccctaat gtcattcaca ttgaatgggg 3661 atgaggtcgg acagtggctc atagagccga gtatgagccc tagctgtggg ctagaaatgt 3721 ccttaataaa catccttatt tttctgttta aaaaaaaaaa aaaaaaaaaa aaaaaaaa //