LOCUS       BC035608                3700 bp    mRNA    linear   HUM 01-SEP-2006
DEFINITION  Homo sapiens nidogen 2 (osteonidogen), mRNA (cDNA clone MGC:45169
            IMAGE:5205293), complete cds.
ACCESSION   BC035608
VERSION     BC035608.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2002) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Life Technologies, Inc.
            cDNA Library Preparation: Life Technologies, Inc.
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: National Institutes of Health Intramural
            Sequencing Center (NISC),
            Gaithersburg, Maryland;
            Web site: http://www.nisc.nih.gov/
            Contact: nisc_mgc@nhgri.nih.gov
            Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
            Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
            Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
            Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
            Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
            McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
            Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
            Young,A., Zhang,L.-H. and Green,E.D.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAK Plate: 68 Row: n Column: 11
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 33469988.
FEATURES             Location/Qualifiers
     source          1..3700
                     /db_xref="H-InvDB:HIT000051438"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:45169 IMAGE:5205293"
                     /tissue_type="Lung, Spleen, fetal, pooled"
                     /clone_lib="NIH_MGC_122"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            1..3700
                     /gene="NID2"
                     /db_xref="GeneID:22795"
                     /db_xref="HGNC:HGNC:13389"
                     /db_xref="MIM:605399"
     CDS             55..2964
                     /gene="NID2"
                     /codon_start=1
                     /product="NID2 protein"
                     /protein_id="AAH35608.1"
                     /db_xref="GeneID:22795"
                     /db_xref="HGNC:HGNC:13389"
                     /db_xref="MIM:605399"
                     /translation="MEGDRVAGRPVLSSLPVLLLLQLLMLRAAALHPDELFPHGESWG
                     DQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTD
                     FPAIAPFLADIDTSHGRGRVLYRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAY
                     TAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGE
                     ETVRITQTAEGLDPENYLSIKTNIQGQVPYVPANFTAHISPYKELYHYSDSTVTSTSS
                     RDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVL
                     RFAVTNQIGPVKEDSDPTPVNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRN
                     CVDENECATGFHRCGPNSVCINLPGSYRCECRSAYEFADDRHTCILITPPANPCEDGS
                     HTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSF
                     SCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPL
                     QCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHY
                     GGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTV
                     APPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVG
                     IDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTD
                     SVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDG
                     ENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFS
                     IVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK"
BASE COUNT          995 a          988 c          888 g          829 t
ORIGIN      
        1 tggaggccgg ggcgggacgc gttgtgcagc gggtaagcgc acggccgagc gagcatggag
       61 ggggaccggg tggccgggcg gccggtgctg tcgtcgttac cagtgctact gctgctgcag
      121 ttgctaatgt tgcgggccgc ggcgctgcac ccagacgagc tcttcccaca cggggagtcg
      181 tggggggacc agctcctgca ggaaggcgac gacgaaagct cagccgtggt gaagctggcg
      241 aatcccctgc acttctacga agcccgattc agcaacctct acgtgggcac caacggcatc
      301 atctccactc aggacttccc cagggaaacg cagtatgtgg actatgattt ccccaccgac
      361 ttcccggcca tcgccccttt tctggcggac atcgacacga gccacggcag aggccgagtc
      421 ctgtaccgag tgaatgggaa agtgagtggc cacctccacg tgggccatac acccgtgcac
      481 ttcactgatg tggacctgca tgcgtatatc gtgggcaatg atggcagagc ctacacggcc
      541 atcagccaca tcccacagcc agcagcccag gccctcctcc ccctcacacc aattggaggc
      601 ctgtttggct ggctctttgc tttagaaaaa cctggctctg agaacggctt cagcctcgca
      661 ggtgctgcct ttacccatga catggaagtt acattctacc cgggagagga gacggttcgt
      721 atcactcaaa ctgctgaggg acttgaccca gagaactacc tgagcattaa gaccaacatt
      781 caaggccagg tgccttacgt cccagcaaat ttcacagccc acatctctcc ctacaaggag
      841 ctgtaccact actccgactc cactgtgacc tctacaagtt ccagagacta ctctctgact
      901 tttggtgcaa tcaaccaaac atggtcctac cgcatccacc agaacatcac ttaccaggtg
      961 tgcaggcatg cccccagaca cccgtccttc cccaccaccc agcagctgaa cgtggaccgg
     1021 gtctttgcct tgtataatga cgaagaaaga gtgcttagat ttgctgtgac caatcaaatt
     1081 ggcccggtca aagaagattc agaccccact ccggtgaatc cttgctatga tgggagccac
     1141 atgtgtgaca caacagcacg gtgccatcca gggacaggtg tagattacac ctgtgagtgc
     1201 gcatctgggt accagggaga tggacggaac tgtgtggatg aaaatgaatg tgcaactggc
     1261 tttcatcgct gtggccccaa ctctgtatgt atcaacttgc ctggaagcta caggtgtgag
     1321 tgccggagtg cttatgagtt tgcagatgac cggcatactt gcatcttgat caccccacct
     1381 gccaacccct gtgaggatgg cagtcatacc tgtgctcctg ctgggcaggc ccggtgtgtt
     1441 caccatggag gcagcacgtt cagctgtgcc tgcctgcctg gttatgccgg cgatgggcac
     1501 cagtgcactg atgtagatga atgctcagaa aacagatgtc accctgcagc tacctgctac
     1561 aatactcctg gttccttctc ctgccgttgt caacccggat attatgggga tggatttcag
     1621 tgcatacctg actccacctc aagcctgaca ccctgtgaac aacagcagcg ccatgcccag
     1681 gcccagtatg cctaccctgg ggcccggttc cacatccccc aatgcgacga gcagggcaac
     1741 ttcctgcccc tacagtgtca tggcagcact ggtttctgct ggtgcgtgga ccctgatggt
     1801 catgaagttc ctggtaccca gactccacct ggctccaccc cgcctcactg tggaccatca
     1861 ccagagccca cccagaggcc cccgaccatc tgtgagcgct ggagggaaaa cctgctggag
     1921 cactacggtg gcaccccccg ggatgaccag tacgtgcccc agtgcgatga cctgggccac
     1981 ttcatccccc tgcagtgcca cggaaagagc gacttctgct ggtgtgtgga caaagatggc
     2041 agagaggtgc agggcacccg ctcccagcca ggcaccaccc ctgcgtgtat acccaccgtc
     2101 gctccaccca tggtccggcc cacgccccgg ccagatgtga cccctccatc tgtgggcacc
     2161 ttcctgctct atactcaggg ccagcagatt ggctacttac ccctcaatgg caccaggctt
     2221 cagaaggatg cagctaagac cctgctgtct ctgcatggct ccataatcgt gggaattgat
     2281 tacgactgcc gggagaggat ggtgtactgg acagatgttg ctggacggac aatcagccgt
     2341 gccggtctgg aactgggagc agagcctgag acgatcgtga attcaggtct gataagccct
     2401 gaaggacttg ccatagacca catccgcaga acaatgtact ggacggacag tgtcctggat
     2461 aagatagaga gcgccctgct ggatggctct gagcgcaagg tcctcttcta cacagatctg
     2521 gtgaatcccc gtgccatcgc tgtggatcca atccgaggca acttgtactg gacagactgg
     2581 aatagagaag ctcctaaaat tgaaacgtca tctttagatg gagaaaacag aagaattctg
     2641 atcaatacag acattggatt gcccaatggc ttaacctttg accctttctc taaactgctc
     2701 tgctgggcag atgcaggaac caaaaaactg gagtgtacac tacctgatgg aactggacgg
     2761 cgtgtcattc aaaacaacct caagtacccc ttcagcatcg taagctatgc agatcacttc
     2821 taccacacag actggaggag ggatggtgtt gtatcagtaa ataaacatag tggccagttt
     2881 actgatgagt atctcccaga acaacgatct cacctctacg ggataactgc agtctacccc
     2941 tactgcccaa caggaagaaa gtaagtacag taatgtaaag gaagacttgg agtttacaat
     3001 cagaacctgg accctaaaga acagtgactg caaaggcaaa gaaagtaaaa aaggaattgg
     3061 ccattagacg ttcctgagca tccaagatga acattttgta gtgcaaaaag acttttgtga
     3121 aaagctgata cctcaatctt tactactgta tttttaaaaa tgaaggttgt tattgcaagt
     3181 ttaaaaaggt aacagaattt taactgttgc ttattaaagc aacttcttgt aaacatttat
     3241 cattaatatt taaaagatca aattcattca actaagaatt agagtttaag actctaaacc
     3301 tgatttttgc catggattcc ttctggccaa gaaattaaag cacatgtgat caatataaca
     3361 atataatcct aaaccttgac agttggagaa gccaatgcag aactgatggg aaaggaccaa
     3421 ttatttatag tttcccaaca aaagttctaa gattttttac ctctgcatca gtgcatttct
     3481 atttatatca aaaggtgcta aaatgattca atttgcattt tctgatcctg tagtgcctct
     3541 atagaagtac ccacagaaag taaagtatca catttataaa taccaaagat gtaacaattt
     3601 taaaattttc tagattactc caataaagtg ttttaagttt tcctatgaaa aaaaaaaaaa
     3661 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//