LOCUS BC032584 1945 bp mRNA linear HUM 07-OCT-2003
DEFINITION Homo sapiens serine hydroxymethyltransferase 2 (mitochondrial),
mRNA (cDNA clone MGC:45240 IMAGE:5499481), complete cds.
ACCESSION BC032584
VERSION BC032584.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1945)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1945)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Lou Staudt
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 69 Row: g Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 19923314.
FEATURES Location/Qualifiers
source 1..1945
/db_xref="H-InvDB:HIT000041618"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:45240 IMAGE:5499481"
/tissue_type="Lymph, lymphoma"
/clone_lib="NIH_MGC_85"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1945
/gene="SHMT2"
/gene_synonym="GLYA"
/gene_synonym="SHMT"
/db_xref="GeneID:6472"
/db_xref="MIM:138450"
CDS 80..1564
/gene="SHMT2"
/gene_synonym="GLYA"
/gene_synonym="SHMT"
/codon_start=1
/product="SHMT2 protein"
/protein_id="AAH32584.1"
/db_xref="GeneID:6472"
/db_xref="MIM:138450"
/translation="MLYFSLFWAARPLQRCGQLVRMAIRAQHSNAAQTQTGEANRGWT
GQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPG
KRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAVYTALLQPHDR
IMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNLALTARLFRPRLIIAGTSAY
ARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGAR
SGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACT
PMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLEL
VSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKS
KTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH"
misc_feature 224..1393
/gene="SHMT2"
/gene_synonym="GLYA"
/gene_synonym="SHMT"
/note="Region: Serine hydroxymethyltransferase"
/db_xref="CDD:pfam00464"
BASE COUNT 424 a 541 c 553 g 427 t
ORIGIN
1 ccacgcgtcc gcttcttccg acagcttgct gccctagacc agagttggtg gctggacctc
61 ctgcgacttc cgagttgcga tgctgtactt ctctttgttt tgggcggctc ggcctctgca
121 gagatgtggg cagctggtca ggatggccat tcgggctcag cacagcaacg cagcccagac
181 tcagactggg gaagcaaaca ggggctggac aggccaggag agcctgtcgg acagtgatcc
241 tgagatgtgg gagttgctgc agagggagaa ggacaggcag tgtcgtggcc tggagctcat
301 tgcctcagag aacttctgca gccgagctgc gctggaggcc ctggggtcct gtctgaacaa
361 caagtactcg gagggttatc ctggcaagag atactatggg ggagcagagg tggtggatga
421 aattgagctg ctgtgccagc gccgggcctt ggaagccttt gacctggatc ctgcacagtg
481 gggagtcaat gtccagccct actccgggtc cccagccaac ctggccgtct acacagccct
541 tctgcaacct cacgaccgga tcatggggct ggacctgccc gatgggggcc atctcaccca
601 cggctacatg tctgacgtca agcggatatc agccacgtcc atcttcttcg agtctatgcc
661 ctataagctc aacctggcac tgactgctcg acttttccgg ccacggctca tcatagctgg
721 caccagcgcc tatgctcgcc tcattgacta cgcccgcatg agagaggtgt gtgatgaagt
781 caaagcacac ctgctggcag acatggccca catcagtggc ctggtggctg ccaaggtgat
841 tccctcgcct ttcaagcacg cggacatcgt caccaccact actcacaaga ctcttcgagg
901 ggccaggtca gggctcatct tctaccggaa aggggtgaag gctgtggacc ccaagactgg
961 ccgggagatc ccttacacat ttgaggaccg aatcaacttt gccgtgttcc catccctgca
1021 ggggggcccc cacaatcatg ccattgctgc agtagctgtg gccctaaagc aggcctgcac
1081 ccccatgttc cgggagtact ccctgcaggt tctgaagaat gctcgggcca tggcagatgc
1141 cctgctagag cgaggctact cactggtatc aggtggtact gacaaccacc tggtgctggt
1201 ggacctgcgg cccaagggcc tggatggagc tcgggctgag cgggtgctag agcttgtatc
1261 catcactgcc aacaagaaca cctgtcctgg agaccgaagt gccatcacac cgggcggcct
1321 gcggcttggg gccccagcct taacttctcg acagttccgt gaggatgact tccggagagt
1381 tgtggacttt atagatgaag gggtcaacat tggcttagag gtgaagagca agactgccaa
1441 gctccaggat ttcaaatcct tcctgcttaa ggactcagaa acaagtcagc gtctggccaa
1501 cctcaggcaa cgggtggagc agtttgccag ggccttcccc atgcctggtt ttgatgagca
1561 ttgaaggcac ctgggaaatg aggcccacag actcaaagtt actctccttc cccctacctg
1621 ggccagtgaa atagaaagcc tttctatttt ttggtgcggg agggaagacc tctcacttag
1681 ggcaagagcc aggtatagtc tcccttccca gaatttgtaa ctgagaagat cttttctttt
1741 tccttttttt ggtaacaaga cttagaagga gggcccaggc actttctgtt tgaacccctg
1801 tcatgatcac agtgtcagag acgcgtcctc tttcttgggg aagttgagga gtgcccttca
1861 gagccagtag caggcagggg tgggtaggca ccctccttcc tgtttttatc taataaaatg
1921 ctaacctgca aaaaaaaaaa aaaaa
//