LOCUS       BC025737                2169 bp    mRNA    linear   HUM 15-JUL-2006
DEFINITION  Homo sapiens pyruvate kinase, liver and RBC, mRNA (cDNA clone
            MGC:34243 IMAGE:5221697), complete cds.
ACCESSION   BC025737
VERSION     BC025737.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2002) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Life Technologies, Inc.
            cDNA Library Preparation: Life Technologies, Inc.
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: National Institutes of Health Intramural
            Sequencing Center (NISC),
            Gaithersburg, Maryland;
            Web site: http://www.nisc.nih.gov/
            Contact: nisc_mgc@nhgri.nih.gov
            Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
            Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
            Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
            Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
            Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
            McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
            Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
            Young,A., Zhang,L.-H. and Green,E.D.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAK Plate: 49 Row: k Column: 10
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 32967595.
FEATURES             Location/Qualifiers
     source          1..2169
                     /db_xref="H-InvDB:HIT000039996"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:34243 IMAGE:5221697"
                     /tissue_type="Pancreas, Spleen, adult pooled"
                     /clone_lib="NIH_MGC_120"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            1..2169
                     /gene="PKLR"
                     /gene_synonym="PK1"
                     /gene_synonym="PKL"
                     /gene_synonym="RPK"
                     /db_xref="GeneID:5313"
                     /db_xref="HGNC:9020"
                     /db_xref="MIM:609712"
     CDS             26..1750
                     /gene="PKLR"
                     /gene_synonym="PK1"
                     /gene_synonym="PKL"
                     /gene_synonym="RPK"
                     /codon_start=1
                     /product="pyruvate kinase, liver and RBC"
                     /protein_id="AAH25737.1"
                     /db_xref="GI:19343993"
                     /db_xref="GeneID:5313"
                     /db_xref="HGNC:9020"
                     /db_xref="MIM:609712"
                     /translation="MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASV
                     AQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVE
                     RLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGP
                     EIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIY
                     IDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGV
                     EHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIM
                     VARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVA
                     NAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRD
                     PTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHL
                     CRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTN
                     IMRVLSIS"
BASE COUNT          512 a          635 c          607 g          415 t
ORIGIN      
        1 gcagccccag gcccacactg aaagcatgtc gatccaggag aacatatcat ccctgcagct
       61 tcggtcatgg gtctctaagt cccaaagaga cttagcaaag tccatcctga ttggggctcc
      121 aggagggcca gcggggtatc tgcggcgggc cagtgtggcc caactgaccc aggagctggg
      181 cactgccttc ttccagcagc agcagctgcc agctgctatg gcagacacct tcctggaaca
      241 cctctgccta ctggacattg actccgagcc cgtggctgct cgcagtacca gcatcattgc
      301 caccatcggg ccagcatctc gctccgtgga gcgcctcaag gagatgatca aggccgggat
      361 gaacattgcg cgactcaact tctcccacgg ctcccacgag taccatgctg agtccatcgc
      421 caacgtccgg gaggcggtgg agagctttgc aggttcccca ctcagctacc ggcccgtggc
      481 catcgccctg gacaccaagg gaccggagat ccgcactggg atcctgcagg ggggtccaga
      541 gtcggaagtg gagctggtga agggctccca ggtgctggtg actgtggacc ccgcgttccg
      601 gacgcggggg aacgcgaaca ccgtgtgggt ggactacccc aatattgtcc gggtcgtgcc
      661 ggtggggggc cgcatctaca ttgacgacgg gctcatctcc ctagtggtcc agaaaatcgg
      721 cccagaggga ctggtgaccc aagtggagaa cggcggcgtc ctgggcagcc ggaagggcgt
      781 gaacttgcca ggggcccagg tggacttgcc cgggctgtcc gagcaggacg tccgagacct
      841 gcgcttcggg gtggagcatg gggtggacat cgtctttgcc tcctttgtgc ggaaagccag
      901 cgacgtggct gccgtcaggg ctgctctggg tccggaagga cacggcatca agatcatcag
      961 caaaattgag aaccacgaag gcgtgaagag gtttgatgaa atcctggagg tgagcgacgg
     1021 catcatggtg gcacgggggg acctaggcat cgagatccca gcagagaagg ttttcctggc
     1081 tcagaagatg atgattgggc gctgcaactt ggcgggcaag cctgttgtct gtgccacaca
     1141 gatgctggag agcatgatta ccaagccccg gccaacgagg gcagagacaa gcgatgtcgc
     1201 caatgctgtg ctggatgggg ctgactgcat catgctgtca ggggagactg ccaagggcaa
     1261 cttccctgtg gaagcggtga agatgcagca tgcgattgcc cgggaggcag aggccgcagt
     1321 gtaccaccgg cagctgtttg aggagctacg tcgggcagcg ccactaagcc gtgatcccac
     1381 tgaggtcacc gccattggtg ctgtggaggc tgccttcaag tgctgtgctg ctgccatcat
     1441 tgtgctgacc acaactggcc gctcagccca gcttctgtct cggtaccgac ctcgggcagc
     1501 agtcattgct gtcacccgct ctgcccaggc tgcccgccag gtccacttat gccgaggagt
     1561 cttccccttg ctttaccgtg aacctccaga agccatctgg gcagatgatg tagatcgccg
     1621 ggtgcaattt ggcattgaaa gtggaaagct ccgtggcttc ctccgtgttg gagacctggt
     1681 gattgtggtg acaggctggc gacctggctc cggctacacc aacatcatga gggtgctaag
     1741 catatcctga gacgcccctc ccccctctgg cccagcctac ccttgtaccc catcccttcc
     1801 tccccagtct acgttctcca gcccacaccc ctccaaagcc ccacctttaa gtcctctctt
     1861 ctctattcct gaccctccct acctgaggcc tatctgagac tataactgtc atctagcccc
     1921 ttcgaggttg ccccttcccc atctccattt cacacaggtc ctgaaagtct gtgtccaatt
     1981 atgcactggc cacccaacag caccaattgt acattctctg catccaatct gctcagcagg
     2041 ccctaagatg ccttgagtct ttaatcccaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaa
//
	

LOCUS       HUMPKLR                 2431 bp    mRNA    linear   HUM 07-JAN-1995
DEFINITION  Human pyruvate kinase type L mRNA, complete cds.
ACCESSION   M15465 J03640
VERSION     M15465.1
KEYWORDS    pyruvate kinase.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1383 to 2431)
  AUTHORS   Tani,K., Fujii,H., Tsutsumi,H., Sukegawa,J., Toyoshima,K.,
            Yoshida,M.C., Noguchi,T., Tanaka,T. and Miwa,S.
  TITLE     Human liver type pyruvate kinase: cDNA cloning and chromosomal
            assignment
  JOURNAL   Biochem. Biophys. Res. Commun. 143 (2), 431-438 (1987)
   PUBMED   3566732
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Tani,K., Fujii,H., Nagata,S. and Miwa,S.
  TITLE     Human liver type pyruvate kinase: complete amino acid sequence and
            the expression in mammalian cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85 (6), 1792-1795 (1988)
   PUBMED   3126495
COMMENT     Original source text: Homo sapiens (strain Japanese) adult liver
            cDNA to mRNA.
            Draft entry and clean copy of sequence in [1] were kindly provided
            by K.Tani,01-MAY-1987.  Draft entry and computer readable copy of
            sequence [2] kindly provided by Tani,K. 20-MAY-1988.
FEATURES             Location/Qualifiers
     source          1..2431
                     /db_xref="H-InvDB:HIT000194396"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /strain="Japanese"
                     /db_xref="taxon:9606"
                     /map="1q21"
                     /tissue_type="liver"
                     /dev_stage="adult"
     gene            1..2431
                     /gene="PKLR"
     CDS             69..1700
                     /gene="PKLR"
                     /EC_number="2.7.1.40"
                     /codon_start=1
                     /product="pyruvate kinase"
                     /protein_id="AAA60104.1"
                     /db_xref="GI:189996"
                     /db_xref="GDB:G00-120-294"
                     /translation="MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCL
                     LDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAETIAN
                     VREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAF
                     RTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKISPEGLVTQVENGGVLGSR
                     KGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHG
                     IKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGK
                     PVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHR
                     IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSA
                     QLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIES
                     GKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS"
BASE COUNT          491 a          727 c          708 g          505 t
ORIGIN      77 bp upstream of HaeIII site; chromosome 1q21.
        1 tgcctctgac aacccaacag tgtggagcag acccacagag agggagaccc agagaggtgt
       61 gcagtggcat ggaagggcca gcggggtatc tgcggcgggc cagtgtggcc caactgaccc
      121 aggagctggg cactgccttc ttccagcagc agcagctgcc agctgctatg gcagacacct
      181 tcctggaaca cctctgccta ctggacattg actccgagcc cgtggctgct cgcagtacca
      241 gcatcattgc caccatcggg ccagcatctc gctccgtgga gcgcctcaag gagatgatca
      301 aggccgggat gaacattgcg cgactcaact tctcccacgg ctcccacgag taccatgctg
      361 agaccatagc caacgtccgg gaggcggtgg agagctttgc aggttcccca ctcagctacc
      421 ggcccgtggc catcgccctg gacaccaagg gaccggagat ccgcactggg atcctgcagg
      481 ggggtccaga gtcggaagtg gagctggtga agggctccca ggtgctggtg actgtggacc
      541 ccgcgttccg gacgcggggg aacgcgaaca ccgtgtgggt ggactacccc aatattgtcc
      601 gggtcgtgcc ggtggggggc cgcatctaca ttgacgacgg gctcatctcc ctagtggtcc
      661 agaaaatcag cccagaggga ctggtgaccc aagtggagaa cggcggcgtc ctgggcagcc
      721 ggaagggcgt gaacttgcca ggggcccagg tggacttgcc cgggctgtcc gagcaggacg
      781 tccgagacct gcgcttcggg gtggagcatg gggtggacat cgtctttgcc tcctttgtgc
      841 ggaaagccag cgacgtggct gccgtcaggg ctgctctggg tccggaagga cacggcatca
      901 agatcatcag caaaattgag aaccacgaag gcgtgaagag gtttgatgaa atcctggagg
      961 tgagcgacgg catcatggtg gcacgggggg acctaggcat cgagatccca gcagagaagg
     1021 ttttcctggc tcagaagatg atgattgggc gctgcaactt ggcgggcaag cctgttgtct
     1081 gtgccacaca gatgctggag agcatgatta ccaagccccg gccaacgagg gcagagacaa
     1141 gcgatgtcgc caatgctgtg ctggatgggg ctgactgcat catgctgtca ggggagactg
     1201 ccaagggcaa cttccctgtg gaagcggtga agatgcagca tcggattgcc cgggaggcag
     1261 aggccgcagt gtaccaccgg cagctgtttg aggagctacg tcgggcagcg ccactaagcc
     1321 gtgatcccac tgaggtcacc gccattggtg ctgtggaggc tgccttcaag tgctgtgctg
     1381 ctgccatcat tgtgctgacc acaactggcc gctcagccca gcttctgtct cggtaccgac
     1441 ctcgggcagc agtcattgct gtcacccgct ctgcccaggc tgcccgccag gtccacttat
     1501 gccgaggagt cttccccttg ctttaccgtg aacctccaga agccatctgg gcagatgatg
     1561 tagatcgccg ggtgcaattt ggcattgaaa gtggaaagct ccgtggcttc ctccgtgttg
     1621 gagacctggt gattgtggtg acaggctggc gacctggctc cggctacacc aacatcatga
     1681 gggtgctaag catatcctga gacgcccctc ccccctctgg cccagcctac ccttgtaccc
     1741 catcccttcc tccccagtct acgttctcca gcccacaccc ctccaaagcc ccacctttaa
     1801 gtcctctctt ctctattcct gaccctccct acctgaggcc tatctgagac tataactgtc
     1861 atctagcccc ttcgaggttg ccccttcccc atctccattt cacacaggtc ctgaaagtct
     1921 gtgtccaatt atgcactggc cacccaacag caccaattgt acattctctg catccaatct
     1981 gctcagcagg ccctaagatg ccttgagtct ttaatcccag gtttggctgg ttaattccat
     2041 aaccccaggc atcccatccc ttggggtggg ggagagggga gacagggcaa tcttgtccac
     2101 agtctcccat tctcatatgt agccctcatg ataatctggg catctcgtgc cagggcaggc
     2161 taccccttca tggtgactaa cagttacatg aaagtccacg cttttgggaa aactgggtgg
     2221 gatggatgct ggggagaagt gagggctggg cagctgattt tgtcactgtc ttcacaactc
     2281 gtgctgggct tgtagaccac tgtcctggct gctctcatgc ctgcctgata ccctgcttgg
     2341 tcaaatcccg gctgcttcct tctgcaccca gaaattcctt cccactcatg ttgttcccac
     2401 acacaaacca agagccaaaa atgagtgtgt c
//