LOCUS BC021203 2810 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphofructokinase, muscle, mRNA (cDNA clone
MGC:13291 IMAGE:4100117), complete cds.
ACCESSION BC021203
VERSION BC021203.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2810)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2810)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC021203.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 17 Row: b Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 39725712.
FEATURES Location/Qualifiers
source 1..2810
/db_xref="H-InvDB:HIT000039139"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:13291 IMAGE:4100117"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2810
/gene="PFKM"
/gene_synonym="MGC8699"
/db_xref="GeneID:5213"
/db_xref="HGNC:HGNC:8877"
/db_xref="MIM:232800"
CDS 38..2380
/gene="PFKM"
/gene_synonym="MGC8699"
/codon_start=1
/product="phosphofructokinase, muscle"
/protein_id="AAH21203.1"
/db_xref="GeneID:5213"
/db_xref="HGNC:HGNC:8877"
/db_xref="MIM:232800"
/translation="MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFT
GARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAA
YNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVG
LVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALV
TSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITS
EDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPAC
VVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVR
PPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEE
AGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLEL
MEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRR
VFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGL
VLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAK
AMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQ
WWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV"
BASE COUNT 710 a 642 c 788 g 670 t
ORIGIN
1 cagcggcgga ggagagctaa gactaaaaga gtggatcatg acccatgaag agcaccatgc
61 agccaaaacc ctggggattg gcaaagccat tgctgtctta acctctggtg gagatgccca
121 aggtatgaat gctgctgtca gggctgtggt tcgagttggt atcttcaccg gtgcccgtgt
181 cttctttgtc catgagggtt atcaaggcct ggtggatggt ggagatcaca tcaaggaagc
241 cacctgggag agcgtttcga tgatgcttca gctgggaggc acggtgattg gaagtgcccg
301 gtgcaaggac tttcgggaac gagaaggacg actccgagct gcctacaacc tggtgaagcg
361 tgggatcacc aatctctgtg tcattggggg tgatggcagc ctcactgggg ctgacacctt
421 ccgttctgag tggagtgact tgttgagtga cctccagaaa gcaggtaaga tcacagatga
481 ggaggctacg aagtccagct acctgaacat tgtgggcctg gttgggtcaa ttgacaatga
541 cttctgtggc accgatatga ccattggcac tgactctgcc ctgcatcgga tcatggaaat
601 tgtagatgcc atcactacca ctgcccagag ccaccagagg acatttgtgt tagaagtaat
661 gggccgccac tgtggatacc tggcccttgt cacctctctg tcctgtgggg ccgactgggt
721 ttttattcct gaatgtccac cagatgacga ctgggaggaa cacctttgtc gccgactcag
781 cgagacaagg acccgtggtt ctcgtctcaa catcatcatt gtggctgagg gtgcaattga
841 caagaatgga aaaccaatca cctcagaaga catcaagaat ctggtggtta agcgtctggg
901 atatgacacc cgggttactg tcttggggca tgtgcagagg ggtgggacgc catcagcctt
961 tgacagaatt ctgggcagca ggatgggtgt ggaagcagtg atggcacttt tggaggggac
1021 cccagatacc ccagcctgtg tagtgagcct ctctggtaac caggctgtgc gcctgcccct
1081 catggaatgt gtccaggtga ccaaagatgt gaccaaggcc atggatgaga agaaatttga
1141 cgaagccctg aagctgagag gccggagctt catgaacaac tgggaggtgt acaagcttct
1201 agctcatgtc agacccccgg tatctaagag tggttcgcac acagtggctg tgatgaacgt
1261 gggggctccg gctgcaggca tgaatgctgc tgttcgctcc actgtgagga ttggccttat
1321 ccagggcaac cgagtgctcg ttgtccatga tggtttcgag ggcctggcca aggggcagat
1381 agaggaagct ggctggagct atgttggggg ctggactggc caaggtggct ctaaacttgg
1441 gactaaaagg actctaccca agaagagctt tgaacagatc agtgccaata taactaagtt
1501 taacattcag ggccttgtca tcattggggg ctttgaggct tacacagggg gcctggaact
1561 gatggagggc aggaagcagt ttgatgagct ctgcatccca tttgtggtca ttcctgctac
1621 agtctccaac aatgtccctg gctcagactt cagcgttggg gctgacacag cactcaatac
1681 tatctgcaca acctgtgacc gcatcaagca gtcagcagct ggcaccaagc gtcgggtgtt
1741 tatcattgag actatgggtg gctactgtgg ctacctggct accatggctg gactggcagc
1801 tggggccgat gctgcctaca tttttgagga gcccttcacc attcgagacc tgcaggcaaa
1861 tgttgaacat ctggtgcaaa agatgaaaac aactgtgaaa aggggcttgg tgttaaggaa
1921 tgaaaagtgc aatgagaact ataccactga cttcattttc aacctgtact ctgaggaggg
1981 gaagggcatc ttcgacagca ggaagaatgt gcttggtcac atgcagcagg gtgggagccc
2041 aaccccattt gataggaatt ttgccactaa gatgggcgcc aaggctatga actggatgtc
2101 tgggaaaatc aaagagagtt accgtaatgg gcggatcttt gccaatactc cagattcggg
2161 ctgtgttctg gggatgcgta agagggctct ggtcttccaa ccagtggctg agctgaagga
2221 ccagacagat tttgagcatc gaatccccaa ggaacagtgg tggctgaaac tgaggcccat
2281 cctcaaaatc ctagccaagt acgagattga cttggacact tcagaccatg cccacctgga
2341 gcacatcacc cggaagcggt ccggggaagc ggccgtctaa acctctctgg agtgagggga
2401 atagattacc tgatcatggt cagctcacac cctaataagt ccacatcttc tcagtgtttt
2461 agctgttttt ttcattaggt ttccttttat tctgtacctt gcagccatga ccagttctgg
2521 ccaggagctg gaggagcagg cagtgggtgg gagctccttt taggtagaat ttaacatgac
2581 ttctgcccca gctttatctg tcacacaagg ctgggcacct ctagtgctac tgctagatat
2641 cacttactca gttagaattt tcctaaaaat aagctttatt tatttctttg tgataacaaa
2701 gagtcttggt tcctctacta cttttactac agtgacaaat tgtaactaca ctaataaatg
2761 ccaactggtc actgtgaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//