LOCUS BC019320 1803 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pseudouridylate synthase 1, mRNA (cDNA clone MGC:4359
IMAGE:2822709), complete cds.
ACCESSION BC019320
VERSION BC019320.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1803)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1803)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: c Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 70166644.
FEATURES Location/Qualifiers
source 1..1803
/db_xref="H-InvDB:HIT000038637"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4359 IMAGE:2822709"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1803
/gene="PUS1"
/gene_synonym="MGC11268"
/gene_synonym="MLASA"
/db_xref="GeneID:80324"
/db_xref="HGNC:HGNC:15508"
/db_xref="MIM:608109"
CDS 227..1510
/gene="PUS1"
/gene_synonym="MGC11268"
/gene_synonym="MLASA"
/codon_start=1
/product="pseudouridylate synthase 1"
/protein_id="AAH19320.2"
/db_xref="GeneID:80324"
/db_xref="HGNC:HGNC:15508"
/db_xref="MIM:608109"
/translation="MGLQLRALLGAFGRWTLRLGPRPSCSPRMAGNAEPPPAGAACPQ
DRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQR
NVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKV
WLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQ
DETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREG
LEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLV
LERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLS
TLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD"
BASE COUNT 389 a 487 c 607 g 320 t
ORIGIN
1 gagaggtcag gggtcagcag gagtgaggct ggggcgtcca ggtccgagag gtcaggggtc
61 agctggagag gggctggggc gccggtttcc cggaggtcag gggtcagaag gaacagggct
121 gcagcgtcag ggtccgagag gttaggggtc ggcagaggcg gagctggggc actgggggtc
181 aggggtcggg gatcagggcg gggtcgggtg cactggtagc ctgcgcatgg gcctccagct
241 tcgcgcgctg ttgggagcct tcggacggtg gaccctgcgc ctgggaccgc gtccgtcctg
301 ctcgccgcgc atggccggga acgcggagcc gccgcccgcc ggagccgcat gcccccagga
361 ccggaggtcc tgcagcggcc gggccggggg cgaccgcgtc tgggaggacg gagaacatcc
421 ggcgaagaag ctcaagagcg gtggcgacga ggagcggcgc gagaagccgc ccaagcggaa
481 gatcgtgctg ctcatggcct attcgggcaa gggctaccac ggcatgcaga ggaatgtcgg
541 gtcctcacaa ttcaaaacaa ttgaagatga cttggtgtcc gccctcgtcc ggtcaggctg
601 tattcctgaa aatcatggtg aggacatgag gaaaatgtcc ttccagcgct gcgcccggac
661 agacaagggt gtgtccgcag ccggccaggt ggtatccctg aaggtgtggc tgattgacga
721 cattctagaa aagatcaaca gccaccttcc ctctcacatt cggattctgg gactgaagcg
781 ggtcacgggc gggtttaact ccaagaacag atgtgatgcc aggacctatt gctacctgct
841 gcccacgttt gcctttgcgc acaaggaccg ggacgttcag gatgagacct accgcctgag
901 cgccgagacg ctgcagcagg tcaacaggct cctggcctgc tacaagggca cgcacaactt
961 ccacaatttc acctcgcaga aggggccgca ggatcccagt gcctgccgct acatcctgga
1021 gatgtactgc gaggaaccct ttgtgcggga gggcctggag tttgcggtga tcagggtgaa
1081 gggccagagc ttcatgatgc atcagatccg gaagatggtc ggcctggtgg tggccattgt
1141 gaagggttat gcccctgaga gcgtgctgga gcgcagctgg ggcacagaga aggtggacgt
1201 gcccaaggcg cccggactcg gcctggtcct ggagagggtg cacttcgaga agtacaacca
1261 gcgctttggc aacgatgggc tgcatgagcc gctggactgg gcgcaggagg aaggaaaggt
1321 cgcagccttc aaggaggagc acatctaccc caccatcatc ggcaccgagc gggacgaacg
1381 ctccatggcc cagtggctga gcaccttgcc catccacaac ttcagtgcca ccgctctcac
1441 ggcaggtggc acgggcgcca aggtgcccag tcccctggaa ggcagtgaag gggacggaga
1501 cactgactga ggcgatggga gctgcccacc agagtgcctc tgagcagctc acagtgtgtg
1561 cccagatgtg ccacccctgt gggcagcaag aagctgggat cgctgcagcc atgttttccc
1621 ggccatgccg gcgttgtaac ctcaggacct tcccttgtag gaacagcctt tctcgaatct
1681 gttttcagct cttgcattgc atagatgaac ctcagcatgt aaagaactat ttttttaaag
1741 aagtgatttt cttattaaac aagtacaaat tttgcttagt caaaaaaaaa aaaaaaaaaa
1801 aaa
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