LOCUS BC015948 1472 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 3, mRNA (cDNA
clone MGC:9491 IMAGE:3922929), complete cds.
ACCESSION BC015948
VERSION BC015948.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1472)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1472)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 15 Row: p Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4885544.
FEATURES Location/Qualifiers
source 1..1472
/db_xref="H-InvDB:HIT000037421"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9491 IMAGE:3922929"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1472
/gene="PDK3"
/db_xref="GeneID:5165"
/db_xref="HGNC:HGNC:8811"
/db_xref="MIM:602526"
CDS 177..1397
/gene="PDK3"
/codon_start=1
/product="pyruvate dehydrogenase kinase, isozyme 3"
/protein_id="AAH15948.1"
/db_xref="GeneID:5165"
/db_xref="HGNC:HGNC:8811"
/db_xref="MIM:602526"
/translation="MRLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKT
SYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDP
QVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRIS
FRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPEL
EVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTL
GKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLY
ARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNP
SSEPRDASKYKAKQ"
BASE COUNT 386 a 336 c 357 g 393 t
ORIGIN
1 gtcctggagc tgctgctgct gctgcggctg ctgcggcggc tgcaccggcg gcgccgaggc
61 cgagatcgag gccggggtgc gcgcttcgca aacgtgccct atccgtgcgg cttggctgcg
121 ccagcccttg cggccacccg ggcgtctagg cgggtctgtg cgccgcccgg gcgaggatgc
181 ggctgttccg gtggctgctg aagcagccgg tgcccaagca gatcgagcgc tactcgcgct
241 tttcgccgtc gccgctctcc atcaaacaat tcctggactt cgggagagat aatgcatgtg
301 agaaaacttc atatatgttt ctacgaaagg aacttcctgt gcggctggct aacacaatga
361 gagaagttaa tcttctgccg gataatttac ttaaccgccc ttcagtggga ttggttcaga
421 gttggtatat gcagagtttt cttgaacttt tagaatatga aaataagagc cctgaggatc
481 cacaggtctt ggataacttt ctacaagttc tgattaaagt cagaaataga cacaatgatg
541 tggttcctac aatggcacaa ggagtgattg aatacaagga gaagtttggg tttgatcctt
601 tcattagcac taacatccaa tattttctgg atcggtttta taccaaccgc atctctttcc
661 gcatgcttat taatcagcac acacttctgt ttgggggtga cactaatcct gttcatccta
721 aacacatagg aagtatcgat cccacctgta acgtggcgga tgtggtgaaa gatgcatatg
781 aaacagccaa gatgctgtgt gaacagtatt acctggtagc tccagagctg gaagttgaag
841 aattcaatgc caaagcgcca gacaaaccta ttcaggtggt ttatgtgccc tcacatctgt
901 ttcatatgct atttgagttg ttcaagaact caatgagagc gacagttgaa ctctatgaag
961 acagaaaaga gggctaccct gctgttaaaa ccctcgttac tttgggtaaa gaagacttat
1021 ccattaagat cagtgaccta ggtggtggtg tcccacttcg aaaaatagat cgtcttttta
1081 actacatgta ttctactgct cctagaccca gcctggagcc taccagagct gcccctttgg
1141 ctggatttgg ttatggtttg ccaatttccc gtctgtatgc tagatatttt caaggagatc
1201 tgaaactgta ttccatggaa ggagtgggta ctgatgctgt catttatttg aaggctcttt
1261 caagtgagtc atttgagaga cttccagttt ttaataagtc cgcatggcgc cattacaaga
1321 ccacgcctga agccgatgat tggagcaatc ccagcagtga acccagggat gcttcaaaat
1381 acaaagcaaa acagtaatat accaccttga tttccattac aaagtatctg atttgtctga
1441 ataaaggtgt cccactcaaa aaaaaaaaaa aa
//