LOCUS BC014634 1884 bp mRNA linear HUM 12-NOV-2003
DEFINITION Homo sapiens fatty acid synthase, mRNA (cDNA clone IMAGE:3535581),
partial cds.
ACCESSION BC014634
VERSION BC014634.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1884)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1884)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (24-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC014634.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: a Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..1884
/db_xref="H-InvDB:HIT000088459"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3535581"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..1884
/gene="FASN"
/gene_synonym="FAS"
/gene_synonym="MGC14367"
/gene_synonym="MGC15706"
/gene_synonym="OA-519"
/db_xref="GeneID:2194"
/db_xref="MIM:600212"
CDS <1..1064
/gene="FASN"
/gene_synonym="FAS"
/gene_synonym="MGC14367"
/gene_synonym="MGC15706"
/gene_synonym="OA-519"
/codon_start=3
/product="FASN protein"
/protein_id="AAH14634.2"
/db_xref="GeneID:2194"
/db_xref="MIM:600212"
/translation="SVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELAC
PTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSL
ASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMC
SQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFT
DMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYT
PKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLES
IISIIHSSLAEPRVSVREG"
misc_feature 252..977
/gene="FASN"
/gene_synonym="FAS"
/gene_synonym="MGC14367"
/gene_synonym="MGC15706"
/gene_synonym="OA-519"
/note="Thioesterase; Region: Thioesterase domain. Peptide
synthetases are involved in the non-ribosomal synthesis of
peptide antibiotics. Next to the operons encoding these
enzymes, in almost all cases, are genes that encode
proteins that have similarity to the type II fatty acid
thioesterases of vertebrates. There are also modules
within the peptide synthetases that also share this
similarity. With respect to antibiotic production,
thioesterases are required for the addition of the last
amino acid to the peptide antibiotic, thereby forming a
cyclic antibiotic. Thioesterases (non-integrated) have
molecular masses of 25-29 kDa"
/db_xref="CDD:pfam00975"
BASE COUNT 342 a 653 c 551 g 338 t
ORIGIN
1 tgagcgtgga ggtgcgccag acgctggagc gtgagctcaa cctggtgctg tccgtgcgcg
61 aggtgcggca actcacgctc cggaaactgc aggagctgtc ctcaaaggcg gatgaggcca
121 gcgagctggc atgccccacg cccaaggagg atggtctggc ccagcagcag actcagctga
181 acctgcgctc cctgctggtg aacccggagg gccccaccct gatgcggctc aactccgtgc
241 agagctcgga gcggcccctg ttcctggtgc acccaatcga gggctccacc accgtgttcc
301 acagcctggc ctcccggctc agcatcccca cctatggcct gcagtgcacc cgagctgcgc
361 cccttgacag catccacagc ctggctgcct actacatcga ctgcatcagg caggtgcagc
421 ccgagggccc ctaccgcgtg gccggctact cctacggggc ctgcgtggcc tttgaaatgt
481 gctcccagct gcaggcccag cagagcccag cccccaccca caacagcctc ttcctgttcg
541 acggctcgcc cacctacgta ctggcctaca cccagagcta ccgggcaaag ctgaccccag
601 gctgtgaggc tgaggctgag acggaggcca tatgcttctt cgtgcagcag ttcacggaca
661 tggagcacaa cagggtgctg gaggcgctgc tgccgctgaa gggcctagag gagcgtgtgg
721 cagccgccgt ggacctgatc atcaagagcc accagggcct ggaccgccag gagctgagct
781 ttgcggcccg gtccttctac tacaagctgc gtgccgctga gcagtacaca cccaaggcca
841 agtaccatgg caacgtgatg ctactgcgcg ccaagacggg tggcgcctac ggcgaggacc
901 tgggcgcgga ctacaacctc tcccaggtat gcgacgggaa agtatccgtc cacgtcatcg
961 agggtgacca ccgcacgctg ctggagggca gcggcctgga gtccatcatc agcatcatcc
1021 acagctccct ggctgagcca cgcgtgagcg tgcgggaggg ctaggcccgt gcccccgcct
1081 gccaccggag gtcactccac catccccacc ccaccccacc ccacccccgc catgcaacgg
1141 gattgaaggg tcctgccggt gggaccctgt ccggcccagt gccactgccc cccgaggctg
1201 ctagatgtag gtgttaggca tgtcccaccc acccgccgcc tcccacggca cctcggggac
1261 accagagctg ccgacttgga gactcctggt ctgtgaagag ccggtggtgc ccgtgcccgc
1321 aggaactggg ctgggcctcg tgcgcccgtg gggtctgcgc ttggtctttc tgtgcttgga
1381 tttgcatatt tattgcattg ctggtagaga cccccaggcc tgtccaccct gccaagactc
1441 ctcaggcagc gtgtgggtcc cgcactctgc ccccatttcc ccaatgtccc ctgcgggcgc
1501 gggcagccac ccaagcctgc tggctgcggc cccctctcgg ccaggcattg gctcagcccg
1561 ctgagtgggg ggtcgtgggc cagtccccga ggagctgggc ccctgcacag gcacacaggg
1621 cccggccaca cccagcggcc ccccgcacag ccacccgtgg ggtgctgccc ttatgcccgg
1681 cgccgggcac caactccatg tttggtgttt gtctgtgttt gtttttcaag aaatgattca
1741 aattgctgct tggattttga aatttactgt aactgtcagt gtacacgtct ggaccccgtt
1801 tcatttttac accaatttgg taaaaatgct gctctcagcc tcccacaatt aaaccgcatg
1861 tgatctccaa aaaaaaaaaa aaaa
//