LOCUS BC008308 3269 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens cullin 4A, mRNA (cDNA clone MGC:15238 IMAGE:3537176),
complete cds.
ACCESSION BC008308
VERSION BC008308.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3269)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3269)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: j Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 57165423.
FEATURES Location/Qualifiers
source 1..3269
/db_xref="H-InvDB:HIT000033715"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:15238 IMAGE:3537176"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3269
/gene="CUL4A"
/db_xref="GeneID:8451"
/db_xref="HGNC:HGNC:2554"
/db_xref="MIM:603137"
CDS 12..2291
/gene="CUL4A"
/codon_start=1
/product="cullin 4A"
/protein_id="AAH08308.2"
/db_xref="GeneID:8451"
/db_xref="HGNC:HGNC:2554"
/db_xref="MIM:603137"
/translation="MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLV
IKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPML
YKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTY
VLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLL
GMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVIT
YLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQ
ALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKE
SFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFE
AFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHF
KQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRK
LQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSEL
RRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQV
STTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLI
DRDYMERDKDNPNQYHYVA"
BASE COUNT 930 a 686 c 840 g 813 t
ORIGIN
1 ccggcccagc catggcggac gaggccccgc ggaagggcag cttctcggcg ctcgtgggcc
61 gcaccaacgg cctcaccaag cccgcggccc tggccgccgc gcccgccaag ccggggggcg
121 cgggcggctc caagaagctg gtcatcaaga acttccgaga cagacctcgg ctgcccgaca
181 actacacgca ggacacgtgg cggaagctgc acgaggcggt gcgggccgtg cagagcagca
241 cctccatcag gtacaacctc gaggagctct accaggctgt ggaaaatctc tgttctcaca
301 aagtctcccc aatgctctac aagcaactgc gtcaggcctg tgaagaccac gtccaggcac
361 agatccttcc gtttagagaa gactcactag atagtgtttt atttttaaag aagattaaca
421 cgtgctggca ggaccactgc agacaaatga tcatgatcag aagcatcttc ctgttcttgg
481 accgcaccta tgtgctgcag aactccacgc tgccctccat ctgggatatg ggattagaac
541 tgtttagaac ccatattatt agtgataaaa tggttcagag taaaaccatt gatggaatcc
601 tactgctgat cgagcgcgag aggagcggcg aggccgtgga ccggagcctg ttgcggagcc
661 tcctgggcat gctgtctgac ctgcaggtgt ataaagattc atttgaactg aaatttttgg
721 aagagactaa ttgcttatat gctgccgaag gccaaaggtt aatgcaggaa agagaggttc
781 cagaatatct taaccatgta agtaaacgct tagaggaaga gggagacaga gtaatcactt
841 acttggacca cagcacacag aaaccactga ttgcttgtgt ggagaaacag ctattaggag
901 aacatttaac agcaattctg cagaaagggc tcgaccactt actggatgag aacagagtgc
961 cggacctcgc acagatgtac cagctgttca gccgggtgag gggcgggcag caggcgctgc
1021 tgcagcactg gagcgagtac atcaagactt ttggaacagc gatcgtaatc aatcctgaga
1081 aagacaaaga catggtccaa gacctgttgg acttcaagga caaggtggac cacgtgatcg
1141 aggtctgctt ccagaagaat gagcggttcg tcaacctgat gaaggagtcc tttgagacgt
1201 tcatcaacaa gagacccaac aagcctgcag aactgatcgc aaagcatgtg gattcaaagt
1261 taagagcagg caacaaagaa gccacagacg aggagctgga gcggacgttg gacaagatca
1321 tgatcctgtt caggtttatc cacggtaaag atgtctttga agcattttat aaaaaagatt
1381 tggcaaaaag actccttgtt gggaaaagtg cctcagtcga tgctgaaaag tctatgttgt
1441 caaagctcaa gcatgagtgc ggtgcagcct tcaccagcaa gctggaaggc atgttcaagg
1501 acatggagct ttcgaaggac atcatggttc atttcaagca gcatatgcag aatcagagtg
1561 actcaggccc tatagacctc acagtgaaca tactcacaat gggctactgg ccaacataca
1621 cgcccatgga agtgcactta accccagaaa tgattaaact tcaggaagta tttaaggcat
1681 tttatcttgg aaagcacagt ggtcgaaaac ttcagtggca aactactttg ggacatgctg
1741 ttttaaaagc ggagtttaaa gaagggaaga aggaattcca ggtgtccctc ttccagacac
1801 tggtgctcct catgttcaac gagggagatg gcttcagctt tgaggagata aaaatggcca
1861 cggggataga ggatagtgaa ttgcgcagaa cgctgcagtc cctggcctgt ggcaaagcac
1921 gtgtgctgat taaaagtccc aaaggaaagg aagtggaaga tggagacaag ttcattttta
1981 atggagagtt caagcacaag ttgtttagaa taaagatcaa tcaaattcag atgaaggaaa
2041 ctgttgagga acaggttagc accactgaga gagtgtttca ggatagacaa tatcagattg
2101 atgctgctat cgtcagaata atgaagatga gaaagactct tggtcataat cttctagttt
2161 ctgaattata taatcagctg aaatttccag taaagcctgg agatttgaaa aagagaattg
2221 aatctctgat agacagagac tatatggaga gagacaaaga caatccgaat cagtaccact
2281 acgtggcctg acgcatctgc agacggttcc ccttcatgaa acactagaat gtaccctcag
2341 agcaggaagc acacctgtgc catttctggg actctgattg atccagctgt ggacattgga
2401 aggcgaagga agggaggtgg ctcctgggtc atctttcaca aggctcaaga cttcaacctg
2461 cagatgtatc tttttccctc cagtttttcc tctagttctt ttaggcattt aaattgtttc
2521 tgttactctg tgcaaaataa ctttgagatt ggacaagaag atgttactaa agagaagttc
2581 ctttaaaagg tcttgttctt gtgtcaaaaa gctgcaagtt tggtttgttc tcgtgtgtga
2641 tcatgagtgc acaatgaaga agaccctaga tgctgcattt tttagctctg aagattcctt
2701 aggtatccct gaagacagct cgctcagatg atcagcattt agagtgaaaa caagggccct
2761 tcatgggtga acattagaaa gagccagggt tcaaagctgg cgaatggatg acgcacccta
2821 gccactggcc cctctctgtt tcatgtattt ccaaaagttg taaactttga tggctgattt
2881 ttcgtaagtc aggtttctaa gtgagctccc tgaggtgcca aggccatggt gtccgccctg
2941 ctgcgtctgt tcgtcagctg agttccttgt gaatctctgt tttagggttt ggggctagtg
3001 tgtttgtgtt tccattctaa gattgagtct ggcagtccct gtttttttgc attggggtaa
3061 ctgctctttg atttttttta attgcagtat ttgtgtgatt gcaataataa agtttggttt
3121 ggtttttaca gtcatgcgca gggacgatcc ttgttctctg ctgtaaactg taaaaagttt
3181 atggagactt aaagtcttga tgttgtgaag cagaggttat tttgtggaaa gattaaaagg
3241 attttgttgg taaaaaaaaa aaaaaaaaa
//