LOCUS BC007872 1421 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens thymidine kinase 1, soluble, mRNA (cDNA clone
MGC:14441 IMAGE:4303880), complete cds.
ACCESSION BC007872
VERSION BC007872.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1421)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1421)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC007872.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 20 Row: o Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 4507518.
FEATURES Location/Qualifiers
source 1..1421
/db_xref="H-InvDB:HIT000033505"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:14441 IMAGE:4303880"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_46"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1421
/gene="TK1"
/gene_synonym="TK2"
/db_xref="GeneID:7083"
/db_xref="HGNC:HGNC:11830"
/db_xref="MIM:188300"
CDS 28..732
/gene="TK1"
/gene_synonym="TK2"
/codon_start=1
/product="thymidine kinase 1, soluble"
/protein_id="AAH07872.1"
/db_xref="GeneID:7083"
/db_xref="HGNC:HGNC:11830"
/db_xref="MIM:188300"
/translation="MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQ
IAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFF
PDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREA
AYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAA
RKLFAPQQILQCSPAN"
BASE COUNT 283 a 433 c 411 g 294 t
ORIGIN
1 cgggttcgtg aacttcccgg aggcgcaatg agctgcatta acctgcccac tgtgctgccc
61 ggctccccca gcaagacccg ggggcagatc caggtgattc tcgggccgat gttctcagga
121 aaaagcacag agttgatgag acgcgtccgt cgcttccaga ttgctcagta caagtgcctg
181 gtgatcaagt atgccaaaga cactcgctac agcagcagct tctgcacaca tgaccggaac
241 accatggagg cgctgcccgc ctgcctgctc cgagacgtgg cccaggaggc cctgggcgtg
301 gctgtcatag gcatcgacga ggggcagttt ttccctgaca tcgtggagtt ctgcgaggcc
361 atggccaacg ccgggaagac cgtaattgtg gctgcactgg atgggacctt ccagaggaag
421 ccatttgggg ccatcctgaa cctggtgccg ctggccgaga gcgtggtgaa gctgacggcg
481 gtgtgcatgg agtgcttccg ggaagccgcc tataccaaga ggctcggcac agagaaggag
541 gtcgaggtga ttgggggagc agacaagtac cactccgtgt gtcggctctg ctacttcaag
601 aaggcctcag gccagcctgc cgggccggac aacaaagaga actgcccagt gccaggaaag
661 ccaggggaag ccgtggctgc caggaagctc tttgccccac agcagattct gcaatgcagc
721 cctgccaact gagggacctg cgagggccgc ccgctccctt cctgccactg ccgcctactg
781 gacgctgccc tgcatgctgc ccagccactc caggaggaag tcgggaggcg tggaggatga
841 ccacaccttg gccttctggg aactctcctt tgtgtggctg ccccacctgc cgcatgctcc
901 ctcctctcct acccactggt ctgcttaaag cttccctctc agctgctggg acgatcgccc
961 aggctggagc tggccccgct tggtggcctg ggatctggca cactccctct ccttggggtg
1021 agggacagag ccccacgctg ttgacatcag cctgcttctt cccctctgcg gctttcactg
1081 ctgagtttct gttctccctg ggaagcctgt gccagcactt ttgagccttg gcccacactg
1141 aggcttaggc ctctctgcct gggatgggct cccaccctcc cctgaggatg gcctggattc
1201 acgccctctt gtttcctttt gggctcaaag cccttcctac ctctggtgat ggtttccaca
1261 ggaacaacag catctttcac caagatgggt ggcaccaacc ttgctgggac ttggatccca
1321 ggggcttatc tcttcaagtg tggagagggc agggtccacg cctctgctgt agcttatgaa
1381 attaactaat tgaaaattca aaaaaaaaaa aaaaaaaaaa a
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