LOCUS BC007798 2661 bp mRNA linear HUM 11-DEC-2003
DEFINITION Homo sapiens phosphofructokinase, muscle, mRNA (cDNA clone
IMAGE:4124137), partial cds.
ACCESSION BC007798
VERSION BC007798.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2661)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2661)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (11-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Dec 9, 2003 this sequence version replaced BC007798.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 20 Row: e Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2661
/db_xref="H-InvDB:HIT000086919"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:4124137"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_19"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2661
/gene="PFKM"
/db_xref="GeneID:5213"
/db_xref="MIM:232800"
CDS <1..2245
/gene="PFKM"
/codon_start=2
/product="PFKM protein"
/protein_id="AAH07798.2"
/db_xref="GeneID:5213"
/db_xref="MIM:232800"
/translation="HEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGA
RVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYN
LVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLV
GSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTS
LSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSED
IKNGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKF
DEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRI
GLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISA
NITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVG
ADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEP
FTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKN
VLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRK
RALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRK
RSGEAAV"
misc_feature 32..2224
/gene="PFKM"
/note="Eukaryotic_PFK; Region: Phosphofructokinase, a key
regulatory enzyme in glycolysis, catalyzes the
phosphorylation of fructose-6-phosphate to
fructose-1,6-biphosphate. The members belong to a
subfamily of the PFKA family (cd00363) and include
eukaryotic ATP-dependent phosphofructokinases. These have
evolved from the bacterial PFKs by gene duplication and
fusion events and exhibit complex allosteric behavior"
/db_xref="CDD:cd00764"
BASE COUNT 664 a 616 c 740 g 641 t
ORIGIN
1 ccatgaagag caccatgcag ccaaaaccct ggggattggc aaagccattg ctgtcttaac
61 ctctggtgga gatgcccaag gtatgaatgc tgctgtcagg gctgtggttc gagttggtat
121 cttcaccggt gcccgtgtct tctttgtcca tgagggttat caaggcctgg tggatggtgg
181 agatcacatc aaggaagcca cctgggagag cgtttcgatg atgcttcagc tgggaggcac
241 ggtgattgga agtgcccggt gcaaggactt tcgggaacga gaaggacgac tccgagctgc
301 ctacaacctg gtgaagcgtg ggatcaccaa tctctgtgtc attgggggtg atggcagcct
361 cactggggct gacaccttcc gttctgagtg gagtgacttg ttgagtgacc tccagaaagc
421 aggtaagatc acagatgagg aggctacgaa gtccagctac ctgaacattg tgggcctggt
481 tgggtcaatt gacaatgact tctgtggcac cgatatgacc attggcactg actctgccct
541 gcatcggatc atggaaattg tagatgccat cactaccact gcccagagcc accagaggac
601 atttgtgtta gaagtaatgg gccgccactg tggatacctg gcccttgtca cctctctgtc
661 ctgtggggcc gactgggttt ttattcctga atgtccacca gatgacgact gggaggaaca
721 cctttgtcgc cgactcagcg agacaaggac ccgtggttct cgtctcaaca tcatcattgt
781 ggctgagggt gcaattgaca agaatggaaa accaatcacc tcagaagaca tcaagaatgg
841 cagcaggatg ggtgtggaag cagtgatggc acttttggag gggaccccag ataccccagc
901 ctgtgtagtg agcctctctg gtaaccaggc tgtgcgcctg cccctcatgg aatgtgtcca
961 ggtgaccaaa gatgtgacca aggccatgga tgagaagaaa tttgacgaag ccctgaagct
1021 gagaggccgg agcttcatga acaactggga ggtgtacaag cttctagctc atgtcagacc
1081 cccggtatct aagagtggtt cgcacacagt ggctgtgatg aacgtggggg ctccggctgc
1141 aggcatgaat gctgctgttc gctccactgt gaggattggc cttatccagg gcaaccgagt
1201 gctcgttgtc catgatggtt tcgagggcct ggccaagggg cagatagagg aagctggctg
1261 gagctatgtt gggggctgga ctggccaagg tggctctaaa cttgggacta aaaggactct
1321 acccaagaag agctttgaac agatcagtgc caatataact aagtttaaca ttcagggcct
1381 tgtcatcatt gggggctttg aggcttacac agggggcctg gaactgatgg agggcaggaa
1441 gcagtttgat gagctctgca tcccatttgt ggtcattcct gctacagtct ccaacaatgt
1501 ccctggctca gacttcagcg ttggggctga cacagcactc aatactatct gcacaacctg
1561 tgaccgcatc aagcagtcag cagctggcac caagcgtcgg gtgtttatca ttgagactat
1621 gggtggctac tgtggctacc tggctaccat ggctggactg gcagctgggg ccgatgctgc
1681 ctacattttt gaggagccct tcaccattcg agacctgcag gcaaatgttg aacatctggt
1741 gcaaaagatg aaaacaactg tgaaaagggg cttggtgtta aggaatgaaa agtgcaatga
1801 gaactatacc actgacttca ttttcaacct gtactctgag gaggggaagg gcatcttcga
1861 cagcaggaag aatgtgcttg gtcacatgca gcagggtggg agcccaaccc catttgatag
1921 gaattttgcc actaagatgg gcgccaaggc tatgaactgg atgtctggga aaatcaaaga
1981 gagttaccgt aatgggcgga tctttgccaa tactccagat tcgggctgtg ttctggggat
2041 gcgtaagagg gctctggtct tccaaccagt ggctgagctg aaggaccaga cagattttga
2101 gcatcgaatc cccaaggaac agtggtggct gaaactgagg cccatcctca aaatcctagc
2161 caagtacgag attgacttgg acacttcaga ccatgcccac ctggagcaca tcacccggaa
2221 gcggtccggg gaagcggccg tctaaacctc tctggagtga ggggaataga ttacctgatc
2281 atggtcagct cacaccctaa taagtccaca tcttctcagt gttttagctg tttttttcat
2341 taggtttcct tttattctgt accttgcagc catgaccagt tctggccagg agctggagga
2401 gcaggcagtg ggtgggagct ccttttaggt agaatttaac atgacttctg ccccagcttt
2461 atctgtcaca caaggctggg cacctctagt gctactgcta gatatcactt actcagttag
2521 aattttccta aaaataagct ttatttattt ctttgtgata acaaagagtc ttggttcctc
2581 tactactttt actacagtga caaattgtaa ctacactaat aaatgccaac tggtcactgt
2641 gaaaaaaaaa aaaaaaaaaa a
//