LOCUS BC007649 2643 bp mRNA linear HUM 25-NOV-2003
DEFINITION Homo sapiens agrin, mRNA (cDNA clone IMAGE:3544662), partial cds.
ACCESSION BC007649
VERSION BC007649.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2643)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2643)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Steven Jones, Jennifer Asano, Ian Bosdet, Yaron Butterfield,
Susanna Chan, Readman Chiu, Chris Fjell, Erin Garland, Ran Guin,
Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Oliver Lee, Soo
Sen Lee, Victor Ling, Carrie Mathewson, Candice McLeavy, Steven
Ness, Pawan Pandoh, Anna-Liisa Prabhu, Parvaneh Saeedi, Jacqueline
Schein, Duane Smailus, Michael Smith, Lorraine Spence, Jeff Stott,
Michael Thorne, Miranada Tsai, Natasja van den Bosch, Jill Vardy,
George Yang, Scott Zuyderduyn, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 23 Row: m Column: 9.
FEATURES Location/Qualifiers
source 1..2643
/db_xref="H-InvDB:HIT000086878"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:3544662"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene <1..2643
/gene="AGRN"
/gene_synonym="FLJ45064"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
CDS <1..1468
/gene="AGRN"
/gene_synonym="FLJ45064"
/codon_start=2
/product="AGRN protein"
/protein_id="AAH07649.1"
/db_xref="GeneID:375790"
/db_xref="MIM:103320"
/translation="CHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAA
PCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLG
EKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSRE
PVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLA
RAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCV
PREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSN
HFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNT
NRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPK
AYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP"
misc_feature 2..85
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="EGF_CA; Region: Calcium-binding EGF-like domain,
present in a large number of membrane-bound and
extracellular (mostly animal) proteins. Many of these
proteins require calcium for their biological function and
calcium-binding sites have been found to be located at the
N-terminus of particular EGF-like domains"
/db_xref="CDD:cd00054"
misc_feature 332..727
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
misc_feature 791..889
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="EGF_CA; Region: Calcium-binding EGF-like domain,
present in a large number of membrane-bound and
extracellular (mostly animal) proteins. Many of these
proteins require calcium for their biological function and
calcium-binding sites have been found to be located at the
N-terminus of particular EGF-like domains"
/db_xref="CDD:cd00054"
misc_feature 1022..1414
/gene="AGRN"
/gene_synonym="FLJ45064"
/note="laminin_G; Region: Laminin G domain"
/db_xref="CDD:pfam00054"
BASE COUNT 458 a 848 c 847 g 490 t
ORIGIN
1 ctgccatggc ggggccccat gccagaacct ggaggctgga aggttccatt gccagtgccc
61 gcccggccgc gtcggaccaa cctgtgccga tgagaagagc ccctgccagc ccaacccctg
121 ccatggggcg gcgccctgcc gtgtgctgcc cgagggtggt gctcagtgcg agtgccccct
181 ggggcgtgag ggcaccttct gccagacagc ctcggggcag gacggctctg ggcccttcct
241 ggctgacttc aacggcttct cccacctgga gctgagaggc ctgcacacct ttgcacggga
301 cctgggggag aagatggcgc tggaggtcgt gttcctggca cgaggcccca gcggcctcct
361 gctctacaac gggcagaaga cggacggcaa gggggacttc gtgtcgctgg cactgcggga
421 ccgccgcctg gagttccgct acgacctggg caagggggca gcggtcatca ggagcaggga
481 gccagtcacc ctgggagcct ggaccagggt ctcactggag cgaaacggcc gcaagggtgc
541 cctgcgtgtg ggcgacggcc cccgtgtgtt gggggagtcc ccggttccgc acaccgtcct
601 caacctgaag gagccgctct acgtaggggg cgctcccgac ttcagcaagc tggcccgtgc
661 tgctgccgtg tcctctggct tcgacggtgc catccagctg gtctccctcg gaggccgcca
721 gctgctgacc ccggagcacg tgctgcggca ggtggacgtc acgtcctttg caggtcaccc
781 ctgcacccgg gcctcaggcc acccctgcct caatggggcc tcctgcgtcc cgagggaggc
841 tgcctatgtg tgcctgtgtc ccgggggatt ctcaggaccg cactgcgaga aggggctggt
901 ggagaagtca gcgggggacg tggatacctt ggcctttgac gggcggacct ttgtcgagta
961 cctcaacgct gtgaccgaga gcgagaaggc actgcagagc aaccactttg aactgagcct
1021 gcgcactgag gccacgcagg ggctggtgct ctggagtggc aaggccacgg agcgggcaga
1081 ctatgtggca ctggccattg tggacgggca cctgcaactg agctacaacc tgggctccca
1141 gcccgtggtg ctgcgttcca ccgtgcccgt caacaccaac cgctggttgc gggtcgtggc
1201 acatagggag cagagggaag gttccctgca ggtgggcaat gaggcccctg tgaccggctc
1261 ctccccgctg ggcgccacgc agctggacac tgatggagcc ctgtggcttg ggggcctgcc
1321 ggagctgccc gtgggcccag cactgcccaa ggcctacggc acaggctttg tgggctgctt
1381 gcgggatgtg gtggtgggcc ggcacccgct gcacctgctg gaggacgccg tcaccaagcc
1441 agagctgcgg ccctgcccca ccccatgagc tggcaccaga gccccgcgcc cgctgtaatt
1501 attttctatt tttgtaaact tgttgctttt tgatatgatt ttcttgcctg agtgttggcc
1561 ggagggactg ctggcccggc ctcccttccg tccaggcagc cgtgctgcag acagacctag
1621 tgctgaggga tggacaggcg aggtggcagc gtggagggct cggcgtggat ggcagcctca
1681 ggacacacac ccctgcctca aggtgctgag cccccgcctt gcactgcgcc tgccccacgg
1741 tgtccccgcc gggaagcagc cccggctcct gaatcaccct cgctccgtca ggcgggactc
1801 gtgtcccaga gaggaagggg ctgctgaggt ctgatggggc ccttcctccg ggtgacccca
1861 cagggccttt ccaagcccct atttgagctg ctccttcctg tgtgtgctct ggaccctgcc
1921 tcggcctcct gcgccaatac tgtgacttcc aaacaatgtt actgctgggc acagctctgc
1981 gttgctcccg tgctgcctgc gccagcccca ggctgctgag gagcagaggc cagaccaggg
2041 ccgatctggg tgtcctgacc ctcagctggc cctgcccagc caccctggac atgaccgtat
2101 ccctctgcca caccccaggc cctgcgaggg gctatcgaga ggagctcact gtgggatggg
2161 gttgacctct gccgcctgcc tgggtatctg ggcctggcca tggctgtgtt cttcatgtgt
2221 tgattttatt tgacccctgg agtggtgggt ctcatctttc ccatctcgcc tgagagcggc
2281 tgagggctgc ctcactgcaa atcctcccca cagcgtcagt gaaagtcgtc cttgtctcag
2341 aatgaccagg ggccagccag tgtctgacca aggtcaaggg gcaggtgcag aggtggcagg
2401 gatggctccg aagccagaaa tgccttaaac tgcaacgtcc cgtcccttcc ccacccccat
2461 cccatcccca cccccagccc cagcccagtc ctcctaggag caggacccga tgaagcgggc
2521 ggcggtgggg ctgggtgccg tgttactaac tctagtatgt ttctgtgtca atcgctgtga
2581 aataaagtct gaaaacttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2641 aaa
//