LOCUS BC006161 3012 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sphingosine kinase 2, mRNA (cDNA clone MGC:13202
IMAGE:3677636), complete cds.
ACCESSION BC006161
VERSION BC006161.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3012)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3012)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-APR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Louis M. Staudt, M.D., Ph.D.
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 18 Row: j Column: 8
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 21361698.
FEATURES Location/Qualifiers
source 1..3012
/db_xref="H-InvDB:HIT000032640"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:13202 IMAGE:3677636"
/tissue_type="Lymph, Burkitt lymphoma"
/clone_lib="NIH_MGC_8"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..3012
/gene="SPHK2"
/db_xref="GeneID:56848"
/db_xref="HGNC:HGNC:18859"
/db_xref="MIM:607092"
CDS 367..2331
/gene="SPHK2"
/codon_start=1
/product="sphingosine kinase 2"
/protein_id="AAH06161.1"
/db_xref="GeneID:56848"
/db_xref="HGNC:HGNC:18859"
/db_xref="MIM:607092"
/translation="MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPL
AASTPLLHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCT
LRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCL
LRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLI
QTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPC
GSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLS
VAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAH
SLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG
PELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARV
GASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLV
HLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGE
QVEYGPLQAQMHPGIGTLLTGPPGCPGREP"
BASE COUNT 522 a 1017 c 934 g 539 t
ORIGIN
1 cgactgacta gccgggcgat aacggcagag agcatagagc gcaggaacaa gcgcaacgtc
61 caagagggaa gggccagcac gtcgggggcc tctctggccc tacccaggcc gtgttctcga
121 tagctttccg gaagaaaggg atctgggagc gagatgcgtg tagctagcac gatgcgtcgc
181 gcggtgacgc tctggcccga cgccgacggc ctctcagtgg ctcccggagg acccggcggg
241 cccagtgttg gagagctgaa ggtcaggcca ggacagtgag acctgactcc ttgctcctac
301 cagcctacta tggcttaaga cccagggcca gggtcccgtt gatgtaacag agcagaggac
361 cagcagatga atggacacct tgaagcagag gagcagcagg accagaggcc agaccaggag
421 ctgaccggga gctggggcca cgggcctagg agcaccctgg tcagggctaa ggccatggcc
481 ccgcccccac cgccactggc tgccagcacc ccgctcctcc atggcgagtt tggctcctac
541 ccagcccgag gcccacgctt tgccctcacc cttacatcgc aggccctgca catacagcgg
601 ctgcgcccca aacctgaagc caggccccgg ggtggcctgg tcccgttggc cgaggtctca
661 ggctgctgca ccctgcgaag ccgcagcccc tcagactcag cggcctactt ctgcatctac
721 acctaccctc ggggccggcg cggggcccgg cgcagagcca ctcgcacctt ccgggcagat
781 ggggccgcca cctacgaaga gaaccgtgcc gaggcccagc gctgggccac tgccctcacc
841 tgtctgctcc gaggactgcc actgcccggg gatggggaga tcacccctga cctgctacct
901 cggccgcccc ggttgcttct attggtcaat ccctttgggg gtcggggcct ggcctggcag
961 tggtgtaaga accacgtgct tcccatgatc tctgaagctg ggctgtcctt caacctcatc
1021 cagacagaac gacagaacca cgcccgggag ctggtccagg ggctgagcct gagtgagtgg
1081 gatggcatcg tcacggtctc gggagacggg ctgctccatg aggtgctgaa cgggctccta
1141 gatcgccctg actgggagga agctgtgaag atgcctgtgg gcatcctccc ctgcggctcg
1201 ggcaacgcgc tggccggagc agtgaaccag cacgggggat ttgagccagc cctgggcctc
1261 gacctgttgc tcaactgctc actgttgctg tgccggggtg gtggccaccc actggacctg
1321 ctctccgtga cgctggcctc gggctcccgc tgtttctcct tcctgtctgt ggcctggggc
1381 ttcgtgtcag atgtggatat ccagagcgag cgcttcaggg ccttgggcag tgcccgcttc
1441 acactgggca cggtgctggg cctcgccaca ctgcacacct accgcggacg cctctcctac
1501 ctccccgcca ctgtggaacc tgcctcgccc acccctgccc atagcctgcc tcgtgccaag
1561 tcggagctga ccctaacccc agacccagcc ccgcccatgg cccactcacc cctgcatcgt
1621 tctgtgtctg acctgcctct tcccctgccc cagcctgccc tggcctctcc tggctcgcca
1681 gaacccctgc ccatcctgtc cctcaacggt gggggcccag agctggctgg ggactggggt
1741 ggggctgggg atgctccgct gtccccggac ccactgctgt cttcacctcc tggctctccc
1801 aaggcagctc tacactcacc cgtctccgaa ggggcccccg taattccccc atcctctggg
1861 ctcccacttc ccacccctga tgcccgggta ggggcctcca cctgcggccc gcccgaccac
1921 ctgctgcctc cgctgggcac cccgctgccc ccagactggg tgacgctgga gggggacttt
1981 gtgctcatgt tggccatctc gcccagccac ctaggcgctg acctggtggc agctccgcat
2041 gcgcgcttcg acgacggcct ggtgcacctg tgctgggtgc gtagcggcat ctcgcgggct
2101 gcgctgctgc gccttttctt ggccatggag cgtggtagcc acttcagcct gggctgtccg
2161 cagctgggct acgccgcggc ccgtgccttc cgcctagagc cgctcacacc acgcggcgtg
2221 ctcacagtgg acggggagca ggtggagtat gggccgctac aggcacagat gcaccctggc
2281 atcggtacac tgctcactgg gcctcctggc tgcccggggc gggagccctg aaactaaaca
2341 agcttggtac ccgccggggg cggggcctac attccaatgg ggcggagcct gagctagggg
2401 gtgtggcctg gctgctagag ttgtggtggc aggggccctg gccccgtctc aggattgcgc
2461 tcgctttcat gggaccagac gtgatgctgg aaggtgggcg tcgtcacggt taaagagaaa
2521 tgggctcgtc ccgagggtag tgcctgatca atgagggcgg ggcctggcgt ctgatctggg
2581 gccgccctta cggggcaggg ctcagtcctg acgcttgcca cctgctccta cccggccagg
2641 atggctgagg gcggagtcta ttttacgcgt cgcccaatga caggacctgg aatgtactgg
2701 ctggggtagg cctcagtgag tcggccggtc agggcccgca gcctcgcccc atccactccg
2761 gtgcctccat ttagctggcc aatcagccca ggaggggcag gttccccggg gccggcgcta
2821 ggatttgcac taatgttcct ctccccgcgg gtgggggcgg ggaaattcat atcccctgtt
2881 cgtctcatgc gcgtcctccg tccccaatct aaaaagcaat tgaaaaggtc tatgcaataa
2941 aggcagtcgc ttcattcctc tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3001 aaaaaaaaaa aa
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