LOCUS BC003584 1133 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens dihydrofolate reductase, mRNA (cDNA clone MGC:3153
IMAGE:3354190), complete cds.
ACCESSION BC003584
VERSION BC003584.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1133)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1133)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 6 Row: n Column: 7
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 68303806.
FEATURES Location/Qualifiers
source 1..1133
/db_xref="H-InvDB:HIT000031619"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:3153 IMAGE:3354190"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1133
/gene="DHFR"
/db_xref="GeneID:1719"
/db_xref="HGNC:HGNC:2861"
/db_xref="MIM:126060"
CDS 455..1018
/gene="DHFR"
/codon_start=1
/product="DHFR protein"
/protein_id="AAH03584.2"
/db_xref="GeneID:1719"
/db_xref="HGNC:HGNC:2861"
/db_xref="MIM:126060"
/translation="MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSV
EGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTE
QPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLL
PEYPGVLSDVQEEKGIKYKFEVYEKND"
BASE COUNT 295 a 263 c 329 g 246 t
ORIGIN
1 aacctactac gtgcggcggc agctggggcg ggaaggcggg cgctgggggc gctgcggccg
61 ctgcagcgcc agggtccacc tggtcggctg cacctgtgga ggaggaggtg gatttcaggc
121 ttcccgtaga ctggaagaat cggctcaaaa ccgcttgcct cgcaggggct gagctggagg
181 cagcgaggcc gcccgacgca ggcttccggc gagacatggc agggcaagga tggcagcccg
241 gcggcagggc ccggcgagga gcgcgaaccc gcggccgcag ttcccaggcg tctgcgggcg
301 cgagcacgcc gcgaccctgc gtgcgccggg gcgggggggc ggggcctcgc ctgcacaaat
361 agggacgagg gggcggggcg gccacaattt cgcgccaaac ttgaccgcgc gttctgctgt
421 aacgagcggg ctcggaggtc ctcccgctgc tgtcatggtt ggttcgctaa actgcatcgt
481 cgctgtgtcc cagaacatgg gcatcggcaa gaacggggac ctgccctggc caccgctcag
541 gaatgaattc agatatttcc agagaatgac cacaacctct tcagtagaag gtaaacagaa
601 tctggtgatt atgggtaaga agacctggtt ctccattcct gagaagaatc gacctttaaa
661 gggtagaatt aatttagttc tcagcagaga actcaaggaa cctccacaag gagctcattt
721 tctttccaga agtctagatg atgccttaaa acttactgaa caaccagaat tagcaaataa
781 agtagacatg gtctggatag ttggtggcag ttctgtttat aaggaagcca tgaatcaccc
841 aggccatctt aaactatttg tgacaaggat catgcaagac tttgaaagtg acacgttttt
901 tccagaaatt gatttggaga aatataaact tctgccagaa tacccaggtg ttctctctga
961 tgtccaggag gagaaaggca ttaagtacaa atttgaagta tatgagaaga atgattaata
1021 tgaaggtgtt ttctagttta agttgttccc cctccctctg aaaaaagtat gtatttttac
1081 attagaaaag gttttttgtt gactttagat ctataaaaaa aaaaaaaaaa aaa
//