LOCUS BC003159 1794 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (RNA) II (DNA directed) polypeptide C,
33kDa, mRNA (cDNA clone MGC:4212 IMAGE:2957867), complete cds.
ACCESSION BC003159
VERSION BC003159.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1794)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1794)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 9 Row: l Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14702172.
FEATURES Location/Qualifiers
source 1..1794
/db_xref="H-InvDB:HIT000031476"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4212 IMAGE:2957867"
/tissue_type="Kidney, renal cell adenocarcinoma"
/clone_lib="NIH_MGC_14"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1794
/gene="POLR2C"
/gene_synonym="hRPB33"
/gene_synonym="hsRPB3"
/gene_synonym="RPB3"
/gene_synonym="RPB31"
/db_xref="GeneID:5432"
/db_xref="HGNC:HGNC:9189"
/db_xref="MIM:180663"
CDS 59..886
/gene="POLR2C"
/gene_synonym="hRPB33"
/gene_synonym="hsRPB3"
/gene_synonym="RPB3"
/gene_synonym="RPB31"
/codon_start=1
/product="polymerase (RNA) II (DNA directed) polypeptide
C, 33kDa"
/protein_id="AAH03159.1"
/db_xref="GeneID:5432"
/db_xref="HGNC:HGNC:9189"
/db_xref="MIM:180663"
/translation="MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPI
IAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFT
LDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELR
LRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQA
PYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN"
BASE COUNT 462 a 446 c 454 g 432 t
ORIGIN
1 cggtggcgcc gcgcagtcac cgcggagcag acgcggaggc tggtggcccc tgggcgagat
61 gccgtacgcc aaccagccta ccgtgcggat cacggagctc actgacgaga atgtcaagtt
121 catcatcgag aacaccgacc tggcggtggc caattcgatt cggagggtct tcatcgctga
181 ggttcccata atagccattg actgggttca gattgatgcc aattcctcag ttcttcatga
241 tgaattcatt gctcacaggc ttggattaat tcccctcatt agtgatgaca ttgtggacaa
301 gctgcagtac tctcgggact gcacatgtga ggagttctgc cccgagtgct cggtggagtt
361 caccctcgat gtgcggtgca atgaagacca gacgcgacat gtcacgtctc gagacctcat
421 ctccaacagc ccccgggtca ttccggtgac atcccggaac cgagataatg accccaatga
481 ctacgtggag caggatgaca tcctcatcgt caagttgaga aagggccagg agctgagact
541 tcgagcctat gccaaaaagg gctttggcaa ggagcatgcc aagtggaacc ctactgcagg
601 ggtggctttt gaatacgatc cagacaatgc cctgaggcac acagtgtacc ccaagcccga
661 ggaatggcca aagagtgagt actcggagct ggatgaggat gagtcgcagg ctccctatga
721 ccccaacggc aagccagaaa ggttttacta caatgtggag tcctgtggct ctctgcgtcc
781 tgaaaccatt gtcctgtcag ccctctcagg attgaagaag aaactgagtg atttacaaac
841 tcaattaagc cacgagatcc agagtgatgt gctaaccata aattaactgc agcttgcctg
901 cttcagcaaa aacggagatt caggccagca gctggatatg ggggtctctc ttcagactct
961 tctcgtttct gagaatctag tctactgttg gttgagcttc ttggcaggac atcagtacca
1021 actagaagtg ggtcatagat agattaccag ggatgcagtg gtgtttaggc aggataggtc
1081 tttactggcc ctgactgctg ttaataattg gcagcagtgc tccccagatc ccagaaggtc
1141 cctgctggag tgtttccagt gcacctgtag ggaaccaact agacttctct cctggttagt
1201 ccagctcttt actctaaacc ctttctgtcc aaaatgagtc attttcagtt gtaccttaga
1261 tgtctggtgt tgaggatcaa gtgccatagc ctttattcag ggggcctata aacccttcca
1321 gttcttgccc cagggctggc ctgctagcgc ttcaaattcc caggtgtccc taatttgaga
1381 agtaaccttt tggaatagca ttagaccctg gctgtcccct ccccaccaaa taaacatgat
1441 atttcattct ctgtccagca gtcatgaacc ccttcacctc caatgacctg atcatttagt
1501 ttggtggggg tgggggtggg ggtgggggtg gaagcagccg caggagcaag ggcccctccc
1561 acatacacag gaggagtatt tcatttctcc ttaatgaagg ctctggccct aacccctcag
1621 cactgtctcc agataggaac atgcacaaag cagttaatta ggcagcctgg agaaaaccag
1681 agatccagta cagaaaggaa aggatattta ttgattaaca gaagttgtct ttttaaaagt
1741 gtttattttt ggcaataaag agcacaacat aatctcaaaa aaaaaaaaaa aaaa
//