LOCUS BC002901 1594 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens pseudouridylate synthase 1, mRNA (cDNA clone MGC:11268
IMAGE:3943243), complete cds.
ACCESSION BC002901
VERSION BC002901.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1594)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1594)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (05-FEB-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC002901.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 15 Row: m Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 70166633.
FEATURES Location/Qualifiers
source 1..1594
/db_xref="H-InvDB:HIT000031276"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:11268 IMAGE:3943243"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1594
/gene="PUS1"
/gene_synonym="MGC11268"
/gene_synonym="MLASA"
/db_xref="GeneID:80324"
/db_xref="HGNC:HGNC:15508"
/db_xref="MIM:608109"
CDS 106..1305
/gene="PUS1"
/gene_synonym="MGC11268"
/gene_synonym="MLASA"
/codon_start=1
/product="pseudouridylate synthase 1"
/protein_id="AAH02901.1"
/db_xref="GeneID:80324"
/db_xref="HGNC:HGNC:15508"
/db_xref="MIM:608109"
/translation="MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSG
GDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENH
GEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTG
GFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFH
NFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAI
VKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEE
GKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGS
EGDGDTD"
BASE COUNT 356 a 446 c 506 g 286 t
ORIGIN
1 gtcgcgaatg gggcaggagc gagcctctct ggtcccgacg cgggtggccc gggtctcctc
61 gactcctgag gaaagcccac cgggcggggc gggagctcgc cgcgcatggc cgggaacgcg
121 gagccgccgc ccgccggagc cgcatgcccc caggaccgga ggtcctgcag cggccgggcc
181 gggggcgacc gcgtctggga ggacggagaa catccggcga agaagctcaa gagcggtggc
241 gacgaggagc ggcgcgagaa gccgcccaag cggaagatcg tgctgctcat ggcctattcg
301 ggcaagggct accacggcat gcagaggaat gtcgggtcct cacaattcaa aacaattgaa
361 gatgacttgg tgtccgccct cgtccggtca ggctgtattc ctgaaaatca tggtgaggac
421 atgaggaaaa tgtccttcca gcgctgcgcc cggacagaca agggtgtgtc cgcagccggc
481 caggtggtat ccctgaaggt gtggctgatt gacgacattc tagaaaagat caacagccac
541 cttccctctc acattcggat tctgggactg aagcgggtca cgggcgggtt taactccaag
601 aacagatgtg atgccaggac ctattgctac ctgctgccca cgtttgcctt tgcgcacaag
661 gaccgggacg ttcaggatga gacctaccgc ctgagcgccg agacgctgca gcaggtcaac
721 aggctcctgg cctgctacaa gggcacgcac aacttccaca atttcacctc gcagaagggg
781 ccgcaggatc ccagtgcctg ccgctacatc ctggagatgt actgcgagga accctttgtg
841 cgggagggcc tggagtttgc ggtgatcagg gtgaagggcc agagcttcat gatgcatcag
901 atccggaaga tggtcggcct ggtggtggcc attgtgaagg gttatgcccc tgagagcgtg
961 ctggagcgca gctggggcac agagaaggtg gacgtgccca aggcgcccgg actcggcctg
1021 gtcctggaga gggtgcactt cgagaagtac aaccagcgct ttggcaacga tgggctgcat
1081 gagccgctgg actgggcgca ggaggaagga aaggtcgcag ccttcaagga ggagcacatc
1141 taccccacca tcatcggcac cgagcgggac gaacgctcca tggcccagtg gctgagcacc
1201 ttgcccatcc acaacttcag tgccaccgct ctcacggcag gtggcacggg cgccaaggtg
1261 cccagtcccc tggaaggcag tgaaggggac ggagacactg actgaggcga tgggagctgc
1321 ccaccagagt gcctctgagc agctcacagt gtgtgcccag atgtgccacc cctgtgggca
1381 gcaagaagct gggatcgctg cagccatgtt ttcccggcca tgccggcgtt gtaacctcag
1441 gaccttccct tgtaggaaca gcctttctcg aatctgtttt cagctcttgc attgcataga
1501 tgaacctcag catgtaaaga actatttttt taaagaagtg attttcttat taaacaagta
1561 caaattttgc ttagtcaaaa aaaaaaaaaa aaaa
//