LOCUS       BC001176                1982 bp    mRNA    linear   HUM 15-JUL-2006
DEFINITION  Homo sapiens transcobalamin II; macrocytic anemia, mRNA (cDNA clone
            MGC:2220 IMAGE:3353271), complete cds.
ACCESSION   BC001176
VERSION     BC001176.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2000) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
            cDNA Library Preparation: Rubin Laboratory
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Genome Sequence Centre,
            BC Cancer Agency, Vancouver, BC, Canada
            info@bcgsc.bc.ca
            Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
            Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
            Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
            Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
            Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
            Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
            Kim MacDonald,  Mike R. Mayo, Josh Moran, Diana Palmquist, JR
            Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
            Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAL Plate: 6 Row: b Column: 1
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 21071009.
FEATURES             Location/Qualifiers
     source          1..1982
                     /db_xref="H-InvDB:HIT000030197"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:2220 IMAGE:3353271"
                     /tissue_type="Eye, retinoblastoma"
                     /clone_lib="NIH_MGC_16"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            1..1982
                     /gene="TCN2"
                     /gene_synonym="D22S676"
                     /gene_synonym="D22S750"
                     /gene_synonym="TC2"
                     /db_xref="GeneID:6948"
                     /db_xref="HGNC:HGNC:11653"
                     /db_xref="MIM:275350"
     CDS             128..1411
                     /gene="TCN2"
                     /gene_synonym="D22S676"
                     /gene_synonym="D22S750"
                     /gene_synonym="TC2"
                     /codon_start=1
                     /product="transcobalamin II; macrocytic anemia"
                     /protein_id="AAH01176.1"
                     /db_xref="GeneID:6948"
                     /db_xref="HGNC:HGNC:11653"
                     /db_xref="MIM:275350"
                     /translation="MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDR
                     LSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSM
                     GQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLG
                     ILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQR
                     ITMAIRTVQEEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLA
                     SLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTL
                     QVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGER
                     EFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW"
BASE COUNT          441 a          598 c          530 g          413 t
ORIGIN      
        1 gattaatcag tgacaggaag ctgcgtctct cggagcggtg accagctgtg gtcaggagag
       61 cctcagcagg gccagcccca ggagtctttc ccgattcttg ctcactgctc acccacctgc
      121 tgctgccatg aggcaccttg gggccttcct cttccttctg ggggtcctgg gggccctcac
      181 tgagatgtgt gaaataccag agatggacag ccatctggta gagaagttgg gccagcacct
      241 cttaccttgg atggaccggc tttccctgga gcacttgaac cccagcatct atgtgggcct
      301 acgcctctcc agtctgcagg ctgggaccaa ggaagacctc tacctgcaca gcctcaagct
      361 tggttaccag cagtgcctcc tagggtctgc cttcagcgag gatgacggtg actgccaggg
      421 caagccttcc atgggccagc tggccctcta cctgctcgct ctcagagcca actgtgagtt
      481 tgtcaggggc cacaaggggg acaggctggt ctcacagctc aaatggttcc tggaggatga
      541 gaagagagcc attgggcatg atcacaaggg ccacccccac actagctact accagtatgg
      601 cctgggcatt ctggccctgt gtctccacca gaagcgggtc catgacagcg tggtggacaa
      661 acttctgtat gctgtggaac ctttccacca gggccaccat tctgtggaca cagcagccat
      721 ggcaggcttg gcattcacct gtctgaagcg ctcaaacttc aaccctggtc ggagacaacg
      781 gatcaccatg gccatcagaa cagtgcaaga ggagatcttg aaggcccaga cccccgaggg
      841 ccactttggg aatgtctaca gcaccccatt ggcattacag ttcctcatga cttcccccat
      901 gcgtggggca gaactgggaa cagcatgtct caaggcgagg gttgctttgc tggccagtct
      961 gcaggatgga gccttccaga atgctctcat gatttcccag ctgctgcccg ttctgaacca
     1021 caagacctac attgatctga tcttcccaga ctgtctggca ccacgagtca tgttggaacc
     1081 agctgctgag accattcctc agacccaaga gatcatcagt gtcacgctgc aggtgcttag
     1141 tctcttgccg ccgtacagac agtccatctc tgttctggcc gggtccaccg tggaagatgt
     1201 cctgaagaag gcccatgagt taggaggatt cacatatgaa acacaggcct ccttgtcagg
     1261 cccctactta acctccgtga tggggaaagc ggccggagaa agggagttct ggcagcttct
     1321 ccgagacccc aacaccccac tgttgcaagg tattgctgac tacagaccca aggatggaga
     1381 aaccattgag ctgaggctgg ttagctggta gcccctgagc tccctcatcc cagcagcctc
     1441 gcacactccc taggcttcta ccctccctcc tgatgtccct ggaacaggaa ctcgcctgac
     1501 cctgctgcca cctcctgtgc actttgagca atgccccctg ggatcacccc agccacaagc
     1561 ccttcgaggg ccctatacca tggcccacct tggagcagag agccaagcat cttccctggg
     1621 aagtctttct ggccaagtct ggccagcctg gccctgcagg tctcccatga aggccacccc
     1681 atggtctgat gggcatgaag catctcagac tccttggcaa aaaacggagt ccgcaggccg
     1741 caggtgttgt gaagaccact cgttctgtgg ttggggtcct gcaagaaggc ctcctcagcc
     1801 cgggggctat ggccctgacc ccagctctcc actctgctgt tagagtggca gctccgagct
     1861 ggttgtggca cagtagctgg ggagacctca gcagggctgc tcagtgcctg cctctgacaa
     1921 aattaaagca ttgatggcct gtggacctgc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1981 aa
//