LOCUS BC001114 1318 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens proteasome (prosome, macropain) subunit, beta type, 8
(large multifunctional peptidase 7), mRNA (cDNA clone MGC:1491
IMAGE:2967119), complete cds.
ACCESSION BC001114
VERSION BC001114.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1318)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1318)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC001114.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 3 Row: m Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34335277.
FEATURES Location/Qualifiers
source 1..1318
/db_xref="H-InvDB:HIT000030145_11"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1491 IMAGE:2967119"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_20"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1318
/gene="PSMB8"
/gene_synonym="D6S216"
/gene_synonym="D6S216E"
/gene_synonym="MGC1491"
/gene_synonym="RING10"
/db_xref="GeneID:5696"
/db_xref="HGNC:HGNC:9545"
/db_xref="MIM:177046"
CDS 239..1057
/gene="PSMB8"
/gene_synonym="D6S216"
/gene_synonym="D6S216E"
/gene_synonym="MGC1491"
/gene_synonym="RING10"
/codon_start=1
/product="proteasome (prosome, macropain) subunit, beta
type, 8 (large multifunctional peptidase 7)"
/protein_id="AAH01114.1"
/db_xref="GeneID:5696"
/db_xref="HGNC:HGNC:9545"
/db_xref="MIM:177046"
/translation="MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGL
EPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKV
IEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRG
MGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPE
EAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ"
BASE COUNT 314 a 337 c 378 g 289 t
ORIGIN
1 agcaacgcta ggaagggcgg gcagaaaggg cacgctcttg tgggtgacta caggttagga
61 gaccgttgaa cctggagggg ccctaggatg gaccccgtgg aaagattcag agactgcgcc
121 ctctccctgg cgccgccttc ccctacacgc ggcgggtata ttctgttgca gttggcccag
181 gacctgtttc caagactctg ccccctcgca cttccgtccc tcctggtttt gtaaagtgat
241 gctcatagga acccccaccc cgcgtgacac tactcccagc tcctggctga cttctagtct
301 tctggttgaa gctgcgcctt tagatgacac gaccctaccc acccctgttt ccagcggatg
361 cccgggcctg gagcccacag aattcttcca gtccctgggt ggggacggag aaaggaacgt
421 tcagattgag atggcccatg gcaccaccac gctcgccttc aagttccagc atggagtgat
481 tgcagcagtg gattctcggg cctcagctgg gtcctacatt agtgccttac gggtgaacaa
541 ggtgattgag attaaccctt acctgcttgg caccatgtct ggctgtgcag cagactgtca
601 gtactgggag cgcctgctgg ccaaggaatg caggctgtac tatctgcgaa atggagaacg
661 tatttcagtg tcggcagcct ccaagctgct gtccaacatg atgtgccagt accggggcat
721 gggcctctct atgggcagta tgatctgtgg ctgggataag aagggtcctg gactctacta
781 cgtggatgaa catgggactc ggctctcagg aaatatgttc tccacgggta gtgggaacac
841 ttatgcctac ggggtcatgg acagtggcta tcggcctaat cttagccctg aagaggccta
901 tgaccttggc cgcagggcta ttgcttatgc cactcacaga gacagctatt ctggaggcgt
961 tgtcaatatg taccacatga aggaagatgg ttgggtgaaa gtagaaagta cagatgtcag
1021 tgacctgctg caccagtacc gggaagccaa tcaataatgg tggtggtggc agctgggcag
1081 gtctcctctg ggaggtcttg gccgactcag ggacctaagc cacgttaagt ccaaggagaa
1141 gaagaggcct agcctgagcc aaagagagag tacgggctca gcagccagag gaggccggtg
1201 aagtgcatct tctgcgtgtt ctctatttga acaagcattt cccccaggga agtttctggg
1261 tgccccacta agtagaataa agaaaaacgg ttataaataa aaaaaaaaaa aaaaaaaa
//