LOCUS BC000629 1633 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens aspartyl-tRNA synthetase, mRNA (cDNA clone MGC:1562
IMAGE:3344322), complete cds.
ACCESSION BC000629
VERSION BC000629.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1633)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1633)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Sep 16, 2003 this sequence version replaced BC000629.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 5 Row: b Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 45439305.
FEATURES Location/Qualifiers
source 1..1633
/db_xref="H-InvDB:HIT000029787"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:1562 IMAGE:3344322"
/tissue_type="Eye, retinoblastoma"
/clone_lib="NIH_MGC_16"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1633
/gene="DARS"
/db_xref="GeneID:1615"
/db_xref="HGNC:HGNC:2678"
/db_xref="MIM:603084"
CDS 50..1555
/gene="DARS"
/codon_start=1
/product="aspartyl-tRNA synthetase"
/protein_id="AAH00629.1"
/db_xref="GeneID:1615"
/db_xref="HGNC:HGNC:2678"
/db_xref="MIM:603084"
/translation="MPSASASRKSQEKPREIMDAAEDYAKERYGISSMIQSQEKPDRV
LVRVRDLTIQKADEVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQMV
KFAANINKESIVDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAV
RPEAEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEI
QTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAED
SNTHRHLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPC
EPFKFLEPTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILD
KYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLE
KIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP"
BASE COUNT 513 a 318 c 382 g 420 t
ORIGIN
1 cggaagcctg cgagggaggg tggtgtccac tgcccagttc cgtgtcccga tgcccagcgc
61 cagcgccagc cgcaagagtc aggagaagcc gcgggagatc atggacgcgg cggaagatta
121 tgctaaagag agatatggaa tatcttcaat gatacaatca caagaaaaac cagatcgagt
181 tttggttcgg gttagagact tgacaataca aaaagctgat gaagttgttt gggtacgtgc
241 aagagttcat acaagcagag ctaaagggaa acagtgcttc ttagtcctac gtcagcagca
301 gtttaatgtc caggctcttg tggcggtggg agaccatgca agcaagcaga tggttaaatt
361 tgctgccaac atcaacaaag agagcattgt ggatgtagaa ggtgttgtga gaaaagtgaa
421 tcagaaaatt ggaagctgta cacagcaaga cgttgagtta catgttcaga agatttatgt
481 gatcagtttg gctgaacccc gtctgcccct gcagctggat gatgctgttc ggcctgaggc
541 agaaggagaa gaggaaggaa gagctactgt taaccaggat acaagattag acaacagagt
601 cattgatctt aggacatcaa ctagtcaggc agtcttccgt ctccagtctg gcatctgcca
661 tctcttccga gaaactttaa ttaacaaagg ttttgtggaa atccaaactc ctaaaattat
721 ttcagctgcc agtgaaggag gagccaatgt ttttactgtg tcatatttta aaaataatgc
781 atacctggct cagtccccac agctatataa gcaaatgtgc atttgtgctg attttgagaa
841 ggttttctct attggaccag tattcagagc ggaagactct aatacccata gacatctaac
901 tgagtttgtt ggtttggaca ttgaaatggc ttttaattac cattaccacg aagttatgga
961 agaaattgct gacaccatgg tacaaatatt caaaggactt caagaaaggt ttcagactga
1021 aattcaaaca gtgaataaac agttcccatg tgagccattc aaatttttgg agccaactct
1081 aagactagaa tattgtgaag cattggctat gcttagggaa gctggagtcg aaatgggaga
1141 tgaagacgat ctgagcacac caaatgaaaa gctgttgggt catttggtaa aggaaaagta
1201 tgatacagat ttttatattc ttgataaata tccattggct gtaagacctt tctataccat
1261 gcctgaccca agaaatccca aacagtccaa ctcttacgat atgttcatga gaggagaaga
1321 aatattgtca ggagctcaaa gaatacatga tcctcaactg ctaacagaga gagctttaca
1381 tcatggaatt gatttggaga aaattaaggc ttacattgat tccttccgct ttggagcccc
1441 tcctcatgct ggtggaggca ttggattgga acgagttact atgctgtttc tgggattgca
1501 taatgttcgt cagacctcca tgttccctcg tgatcccaaa cgactcactc cttaaattca
1561 cactttgcca cttaactcca gtgtggatga cagagcgaga ccctgcctca aaaaaaaaaa
1621 aaaaaaaaaa aaa
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