LOCUS BC000534 2812 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens phosphofructokinase, muscle, mRNA (cDNA clone MGC:8699
IMAGE:2964710), complete cds.
ACCESSION BC000534
VERSION BC000534.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2812)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2812)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000534.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: p Column: 18
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 39725712.
FEATURES Location/Qualifiers
source 1..2812
/db_xref="H-InvDB:HIT000029716"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8699 IMAGE:2964710"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2812
/gene="PFKM"
/gene_synonym="MGC8699"
/db_xref="GeneID:5213"
/db_xref="HGNC:HGNC:8877"
/db_xref="MIM:232800"
CDS 16..2358
/gene="PFKM"
/gene_synonym="MGC8699"
/codon_start=1
/product="phosphofructokinase, muscle"
/protein_id="AAH00534.1"
/db_xref="GeneID:5213"
/db_xref="HGNC:HGNC:8877"
/db_xref="MIM:232800"
/translation="MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFT
GARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAA
YNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVG
LVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALV
TSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITS
EDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPAC
VVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVR
PPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEE
AGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLEL
MEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRR
VFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGL
VLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAK
AMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQ
WWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV"
BASE COUNT 727 a 638 c 777 g 670 t
ORIGIN
1 ctaaaagagt ggatcatgac ccatgaagag caccatgcag ccaaaaccct ggggattggc
61 aaagccattg ctgtcttaac ctctggtgga gatgcccaag gtatgaatgc tgctgtcagg
121 gctgtggttc gagttggtat cttcaccggt gcccgtgtct tctttgtcca tgagggttat
181 caaggcctgg tggatggtgg agatcacatc aaggaagcca cctgggagag cgtttcgatg
241 atgcttcagc tgggaggcac ggtgattgga agtgcccggt gcaaggactt tcgggaacga
301 gaaggacgac tccgagctgc ctacaacctg gtgaagcgtg ggatcaccaa tctctgtgtc
361 attgggggtg atggcagcct cactggggct gacaccttcc gttctgagtg gagtgacttg
421 ttgagtgacc tccagaaagc aggtaagatc acagatgagg aggctacgaa gtccagctac
481 ctgaacattg tgggcctggt tgggtcaatt gacaatgact tctgtggcac cgatatgacc
541 attggcactg actctgccct gcatcggatc atggaaattg tagatgccat cactaccact
601 gcccagagcc accagaggac atttgtgtta gaagtaatgg gccgccactg tggatacctg
661 gcccttgtca cctctctgtc ctgtggggcc gactgggttt ttattcctga atgtccacca
721 gatgacgact gggaggaaca cctttgtcgc cgactcagcg agacaaggac ccgtggttct
781 cgtctcaaca tcatcattgt ggctgagggt gcaattgaca agaatggaaa accaatcacc
841 tcagaagaca tcaagaatct ggtggttaag cgtctgggat atgacacccg ggttactgtc
901 ttggggcatg tgcagagggg tgggacgcca tcagcctttg acagaattct gggcagcagg
961 atgggtgtgg aagcagtgat ggcacttttg gaggggaccc cagatacccc agcctgtgta
1021 gtgagcctct ctggtaacca ggctgtgcgc ctgcccctca tggaatgtgt ccaggtgacc
1081 aaagatgtga ccaaggccat ggatgagaag aaatttgacg aagccctgaa gctgagaggc
1141 cggagcttca tgaacaactg ggaggtgtac aagcttctag ctcatgtcag acccccggta
1201 tctaagagtg gttcgcacac agtggctgtg atgaacgtgg gggctccggc tgcaggcatg
1261 aatgctgctg ttcgctccac tgtgaggatt ggccttatcc agggcaaccg agtgctcgtt
1321 gtccatgatg gtttcgaggg cctggccaag gggcagatag aggaagctgg ctggagctat
1381 gttgggggct ggactggcca aggtggctct aaacttggga ctaaaaggac tctacccaag
1441 aagagctttg aacagatcag tgccaatata actaagttta acattcaggg ccttgtcatc
1501 attgggggct ttgaggctta cacagggggc ctggaactga tggagggcag gaagcagttt
1561 gatgagctct gcatcccatt tgtggtcatt cctgctacag tctccaacaa tgtccctggc
1621 tcagacttca gcgttggggc tgacacagca ctcaatacta tctgcacaac ctgtgaccgc
1681 atcaagcagt cagcagctgg caccaagcgt cgggtgttta tcattgagac tatgggtggc
1741 tactgtggct acctggctac catggctgga ctggcagctg gggccgatgc tgcctacatt
1801 tttgaggagc ccttcaccat tcgagacctg caggcaaatg ttgaacatct ggtgcaaaag
1861 atgaaaacaa ctgtgaaaag gggcttggtg ttaaggaatg aaaagtgcaa tgagaactat
1921 accactgact tcattttcaa cctgtactct gaggagggga agggcatctt cgacagcagg
1981 aagaatgtgc ttggtcacat gcagcagggt gggagcccaa ccccatttga taggaatttt
2041 gccactaaga tgggcgccaa ggctatgaac tggatgtctg ggaaaatcaa agagagttac
2101 cgtaatgggc ggatctttgc caatactcca gattcgggct gtgttctggg gatgcgtaag
2161 agggctctgg tcttccaacc agtggctgag ctgaaggacc agacagattt tgagcatcga
2221 atccccaagg aacagtggtg gctgaaactg aggcccatcc tcaaaatcct agccaagtac
2281 gagattgact tggacacttc agaccatgcc cacctggagc acatcacccg gaagcggtcc
2341 ggggaagctg ccgtctaaac ctctctggag tgaggggaat agattacctg atcatggtca
2401 gctcacaccc taataagtcc acatcttctc agtgttttag ctgttttttt cattaggttt
2461 ccttttattc tgtaccttgc agccatgacc agttctggcc aggagctgga ggagcaggca
2521 gtgggtggga gctcctttta ggtagaattt aacatgactt ctgccccagc tttatctgtc
2581 acacaaggct gggcacctct agtgctactg ctagatatca cttactcagt tagaattttc
2641 ctaaaaataa gctttattta tttctttgtg ataacaaaga gtcttggttc ctctactact
2701 tttactacag tgacaaattg taactacact aataaatgcc aactggtcac tgtgaaaaaa
2761 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
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