LOCUS BC000427 1114 bp mRNA linear HUM 15-JUL-2006 DEFINITION Homo sapiens ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), mRNA (cDNA clone MGC:8352 IMAGE:2819764), complete cds. ACCESSION BC000427 VERSION BC000427.2 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1114) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 1114) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT On Aug 19, 2003 this sequence version replaced BC000427.1. Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: DCTD/DTP cDNA Library Preparation: Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAL Plate: 1 Row: b Column: 7 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 35493995. FEATURES Location/Qualifiers source 1..1114 /db_xref="H-InvDB:HIT000029613" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:8352 IMAGE:2819764" /tissue_type="Lung, small cell carcinoma" /clone_lib="NIH_MGC_7" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 1..1114 /gene="UBE2I" /gene_synonym="C358B7.1" /gene_synonym="P18" /gene_synonym="UBC9" /db_xref="GeneID:7329" /db_xref="HGNC:HGNC:12485" /db_xref="MIM:601661" CDS 63..539 /gene="UBE2I" /gene_synonym="C358B7.1" /gene_synonym="P18" /gene_synonym="UBC9" /codon_start=1 /product="ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)" /protein_id="AAH00427.1" /db_xref="GeneID:7329" /db_xref="HGNC:HGNC:12485" /db_xref="MIM:601661" /translation="MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECA IPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKD WRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS" BASE COUNT 285 a 275 c 268 g 286 t ORIGIN 1 gctcggtcct ccacctgtcc gctacgctcg ccggggctgc ggccgcccga gggactttga 61 acatgtcggg gatcgccctc agcagactcg cccaggagag gaaagcatgg aggaaagacc 121 acccatttgg tttcgtggct gtcccaacaa aaaatcccga tggcacgatg aacctcatga 181 actgggagtg cgccattcca ggaaagaaag ggactccgtg ggaaggaggc ttgtttaaac 241 tacggatgct tttcaaagat gattatccat cttcgccacc aaaatgtaaa ttcgaaccac 301 cattatttca cccgaatgtg tacccttcgg ggacagtgtg cctgtccatc ttagaggagg 361 acaaggactg gaggccagcc atcacaatca aacagatcct attaggaata caggaacttc 421 taaatgaacc aaatatccaa gacccagctc aagcagaggc ctacacgatt tactgccaaa 481 acagagtgga gtacgagaaa agggtccgag cacaagccaa gaagtttgcg ccctcataag 541 cagcgacctt gtggcatcgt caaaaggaag ggattggttt ggcaagaact tgtttacaac 601 atttttgcaa atctaaagtt gctccataca atgactagtc acctgggggg gttgggcggg 661 cgccatcttc cattgccgcc gcgggtgtgc ggtctcgatt cgctgaattg cccgtttcca 721 tacagggtct cttccttcgg tcttttgtat ttttgattgt tatgtaaaac tcgcttttat 781 tttaatattg atgtcagtat ttcaactgct gtaaaattat aaacttttat acttgggtaa 841 gtcccccagg ggcgagttcc tcgctctggg atgcaggcat gcttctcacc gtgcagagct 901 gcacttggcc tcagctggct gtatggaaat gcaccctccc tcctgccgct cctctctaga 961 accttctaga acctgggctg tgctgctttt gagcctcaga ccccagggca gcatctcggt 1021 tctgcgccac ttcctttgtg tttatatggc gttttgtctg tgttgctgtt tagagtaaat 1081 aaactgttta tataaaaaaa aaaaaaaaaa aaaa //