LOCUS BC000409 1422 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens polymerase (RNA) II (DNA directed) polypeptide C,
33kDa, mRNA (cDNA clone MGC:8577 IMAGE:2960943), complete cds.
ACCESSION BC000409
VERSION BC000409.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1422)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1422)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Nov 6, 2003 this sequence version replaced BC000409.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 1 Row: k Column: 16
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 14702172.
FEATURES Location/Qualifiers
source 1..1422
/db_xref="H-InvDB:HIT000029596"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:8577 IMAGE:2960943"
/tissue_type="Muscle, rhabdomyosarcoma"
/clone_lib="NIH_MGC_17"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1422
/gene="POLR2C"
/gene_synonym="hRPB33"
/gene_synonym="hsRPB3"
/gene_synonym="RPB3"
/gene_synonym="RPB31"
/db_xref="GeneID:5432"
/db_xref="HGNC:HGNC:9189"
/db_xref="MIM:180663"
CDS 18..845
/gene="POLR2C"
/gene_synonym="hRPB33"
/gene_synonym="hsRPB3"
/gene_synonym="RPB3"
/gene_synonym="RPB31"
/codon_start=1
/product="polymerase (RNA) II (DNA directed) polypeptide
C, 33kDa"
/protein_id="AAH00409.1"
/db_xref="GeneID:5432"
/db_xref="HGNC:HGNC:9189"
/db_xref="MIM:180663"
/translation="MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPI
IAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFT
LDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELR
LRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQA
PYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN"
BASE COUNT 361 a 361 c 354 g 346 t
ORIGIN
1 ggtggcccct gggcgagatg ccgtacgcca accagcctac cgtgcggatc acggagctca
61 ctgacgagaa tgtcaagttc atcatcgaga acaccgacct ggcggtggcc aattcgattc
121 ggagggtctt catcgctgag gttcccataa tagccattga ctgggttcag attgatgcca
181 attcctcagt ccttcatgat gaattcattg ctcacaggct tggattaatt cccctcatta
241 gtgatgacat tgtggacaag ctgcagtact ctcgggactg cacatgtgag gagttctgcc
301 ccgagtgctc ggtggagttc accctcgatg tgcggtgcaa tgaagaccag acgcgacatg
361 tcacgtctcg agacctcatc tccaacagcc cccgggtcat tccggtgaca tcccggaacc
421 gagataatga ccccaatgac tacgtggagc aggatgacat cctcatcgtc aagttgagaa
481 agggccagga gctgagactt cgagcctatg ccaaaaaggg ctttggcaag gagcatgcca
541 agtggaaccc tactgcaggg gtggcttttg aatacgatcc agacaatgcc ctgaggcaca
601 cagtgtaccc caagcccgag gaatggccaa agagtgagta ctcggagctg gatgaggatg
661 agtcgcaggc tccctatgac cccaacggca agccagaaag gttttactac aatgtggagt
721 cctgtggctc tctgcgtcct gaaaccattg tcctgtcagc cctctcagga ttgaagaaga
781 aactgagtga tttacaaact caattaagcc acgagatcca gagtgatgtg ctaaccataa
841 attaactgca gcttgcctgc ttcagcaaaa acggagattc aggccagcag ctggatatgg
901 gggtctctct tcagactctt ctcgtttctg agaatctagt ctactgttgg ttgagcttct
961 tggcaggaca tcagtaccaa ctagaagtgg gtcatagata gattaccagg gatgcagtgg
1021 tgtttaggca ggataggtct ttactggccc tgactgctgt taataattgg cagcagtgct
1081 ccccagatcc cagaaggtcc ctgctggagt gtttccagtg cacctgtagg gaaccaacta
1141 gacttctctc ctggttagtc cagctcttta ctctaaaccc tttctgtcca aaatgagtca
1201 ttttcagttg taccttagat gtctggtgtt gaggatcaag tgccatagcc tttattcagg
1261 gggcctataa acccttccag ttcttgcccc agggctggcc tgctagcgct tcaaattccc
1321 aggtgtccct aatttgagaa gtaacctttt ggaatagcat tagaccctgg ctgtcccctc
1381 cccaccaaat aaacatgata tttcaaaaaa aaaaaaaaaa aa
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