LOCUS       BB477598                 285 bp    mRNA    linear   EST 08-SEP-2005
DEFINITION  Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched
            library, clone:D330025M22, 3' end partial sequence, similar to
            ddbj:U81490 Mus musculus polycomblike (mPcl1) mRNA.
ACCESSION   BB477598
VERSION     BB477598.1
KEYWORDS    EST; 3'-end sequence (3'-EST).
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Arakawa,T.,
            Carninci,P., Endo,T., Fukuda,S., Fukunishi,Y., Hara,A.,
            Hayatsu,N., Hirozane,T., Hori,F., Ishii,Y., Ishikawa,J.,
            Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I., Kai,C.,
            Kawai,J., Kikuchi,N., Kiyosawa,H., Kojima,Y., Kondo,S., Koya,S.,
            Kurihara,C., Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y.,
            Nakamura,M., Oda,H., Okazaki,Y., Ono,T., Owa,C., Saito,H.,
            Sakai,C., Sato,K., Shibata,K., Shibata,Y., Shigemoto,Y.,
            Shinagawa,A., Shiraki,T., Sogabe,Y., Sugahara,Y., Suzuki,H.,
            Suzuki,H., Tagawa,A., Takahashi,F., Tominaga,N., Toya,T.,
            Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yamanaka,I.,
            Yano,R., Yasunishi,A., Yokota,T., Yoshida,K., Yoshiki,A.,
            Yoshino,M., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2000) to the DDBJ/EMBL/GenBank databases.
            Contact:Yoshihide Hayashizaki
            The Institute of Physical and Chemical Research (RIKEN), Omics
            Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
            Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
            URL    :http://www.osc.riken.jp/
REFERENCE   2
  AUTHORS   
  CONSRTM   The FANTOM Consortium, Riken Genome Exploration Research Group and
            Genome Science Group (Genome Network Project Core Group)
  TITLE     The Transcriptional Landscape of the Mammalian Genome
  JOURNAL   Science 309, 1559-1563 (2005)
REFERENCE   3
  AUTHORS   
  CONSRTM   RIKEN Genome Exploration Research Group and Genome Science Group
            (Genome Network Project Core Group) and the FANTOM Consortium
  TITLE     Antisense Transcription in the Mammalian Transcriptome
  JOURNAL   Science 309, 1564-1566 (2005)
REFERENCE   4
  AUTHORS   Carninci,P. and Hayashizaki,Y.
  TITLE     High-efficiency full-length cDNA cloning
  JOURNAL   Meth. Enzymol. 303, 19-44 (1999)
REFERENCE   5
  AUTHORS   Carninci,P., Nishiyama,Y., Westover,A., Itoh,M., Nagaoka,S.,
            Sasaki,N., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
  TITLE     Thermostabilization and thermoactivation of thermolabile enzymes
            by trehalose and its application for the synthesis of full length
            cDNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95, 520-524 (1998)
REFERENCE   6
  AUTHORS   Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J.,
            Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M.,
            Okazaki,Y. and Hayashizaki,Y.
  TITLE     Automated filtration-based high-throughput plasmid preparation
            system
  JOURNAL   Genome Res. 9, 463-470 (1999)
COMMENT     Please visit our web site (http://www.osc.riken.jp/) for further
            details.
            cDNA library was prepared and sequenced in Mouse Genome
            Encyclopedia Project of Genome Exploration Research Group in Riken
            Genomic Sciences Center and Genome Science Laboratory in RIKEN.
            Division of Experimental Animal Research in Riken contributed to
            prepare mouse tissues. 1st strand cDNA was primed with a primer
            [5' GAGAGAGAGAGCGGCCGCAACTCGAGTTTTTTTTTTTTTTTTVN 3'], cDNA was
            prepared by using trehalose thermo-activated reverse transcriptase
            and subsequently enriched for full-length by cap-trapper. Second
            strand cDNA was prepared with the primer adapter of sequence [5'
            GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was
            cleaved with BamHI and XhoI. Vector: a modified pBluescript KS(+)
            after bulk excision from Lambda FLC I. Cloning sites, 5' end:
            SalI; 3' end: BamHI. Host: DH10B.
FEATURES             Location/Qualifiers
     source          1..285
                     /clone="D330025M22"
                     /clone_lib="RIKEN full-length enriched mouse cDNA library"
                     /db_xref="taxon:10090"
                     /dev_stage="13 days embryo"
                     /mol_type="mRNA"
                     /organism="Mus musculus"
                     /tissue_type="heart"
BASE COUNT           63 a           78 c           62 g           81 t
ORIGIN      
        1 tcattccctg acctcccccc tcaccaacag cctccacata ccnggagaga tggggagtgg
       61 ttttccctcc tgcccaggct ggaatccagg ggagtgaaga ggtccttttc tctccccctt
      121 catgactcct aatccccccc ctcatttcct tccatgttat tttgttacag cttttgagat
      181 aattttttaa gaccatttaa cccctgggat tagagatcgg gggggggggt ttggctggtc
      241 caggattcca gactgtatga ctgtaataaa gataaataaa cctag
//