LOCUS BB081826 247 bp mRNA linear EST 07-SEP-2005 DEFINITION Mus musculus adult male diencephalon cDNA, RIKEN full-length enriched library, clone:9330171F07, 3' end partial sequence, similar to ddbj:U81490 Mus musculus polycomblike (mPcl1) mRNA. ACCESSION BB081826 VERSION BB081826.1 KEYWORDS EST; 3'-end sequence (3'-EST). SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 247) AUTHORS Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Arakawa,T., Carninci,P., Endo,T., Fukuda,S., Fukunishi,Y., Hara,A., Hayatsu,N., Hirozane,T., Hori,F., Ishii,Y., Ishikawa,J., Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I., Kai,C., Kawai,J., Kikuchi,N., Kiyosawa,H., Kojima,Y., Kondo,S., Koya,S., Kurihara,C., Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y., Nakamura,M., Oda,H., Okazaki,Y., Ono,T., Owa,C., Saito,H., Sakai,C., Sato,K., Shibata,K., Shibata,Y., Shigemoto,Y., Shinagawa,A., Shiraki,T., Sogabe,Y., Sugahara,Y., Suzuki,H., Suzuki,H., Tagawa,A., Takahashi,F., Tominaga,N., Toya,T., Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yamanaka,I., Yano,R., Yasunishi,A., Yokota,T., Yoshida,K., Yoshiki,A., Yoshino,M., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (16-MAY-2000) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) TITLE The Transcriptional Landscape of the Mammalian Genome JOURNAL Science 309, 1559-1563 (2005) REFERENCE 3 AUTHORS CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium TITLE Antisense Transcription in the Mammalian Transcriptome JOURNAL Science 309, 1564-1566 (2005) REFERENCE 4 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency full-length cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) REFERENCE 5 AUTHORS Carninci,P., Nishiyama,Y., Westover,A., Itoh,M., Nagaoka,S., Sasaki,N., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA JOURNAL Proc. Natl. Acad. Sci. U.S.A. 95, 520-524 (1998) REFERENCE 6 AUTHORS Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J., Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M., Okazaki,Y. and Hayashizaki,Y. TITLE Automated filtration-based high-throughput plasmid preparation system JOURNAL Genome Res. 9, 463-470 (1999) COMMENT Please visit our web site (http://www.osc.riken.jp/) for further details. cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 20.0 and subtraction to Rot = 370.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cleaved with XhoI and BamHI. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B. FEATURES Location/Qualifiers source 1..247 /clone="9330171F07" /clone_lib="RIKEN full-length enriched mouse cDNA library" /db_xref="taxon:10090" /dev_stage="adult" /mol_type="mRNA" /organism="Mus musculus" /sex="male" /tissue_type="diencephalon" BASE COUNT 52 a 55 c 62 g 78 t ORIGIN 1 cgtacttcga gagatgggga gtggttctcc ctcctgcccc ggctgggatc caggggagtg 61 aagaggtcct tttctctccc ccttcatgac tcctaatccc ccccctcatt tcctttgatg 121 ttattttgtt acagcttttg agataatttt ttaagactat ttaacccctg ggattagaga 181 ttgggggggg gggtttgggt ggtccaggat tccagactgt atgactgtaa taaagataaa 241 taaacct //